Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees

Autores
Garrido, Paula Melisa; Porrini, Martín Pablo; Antúnez, karina; Branchiccela, Belén; Martínez Noël, Giselle María Astrid; Zunino, Pablo; Salerno, Graciela Lidia; Eguaras, Martin Javier; Ieno, Elena
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control Varroa mites. These compounds can accumulate at high concentrations in apicultural matrices. In this work, the effects of parasitosis/acaricide on genes involved in honeybee immunity and survival were evaluated. Nurse bees were infected with N. ceranae and/or were chronically treated with sublethal doses of coumaphos or tau-fluvalinate, the two most abundant pesticides recorded in productive hives. Our results demonstrate the following: (1) honeybee survival was not affected by any of the treatments; (2) parasite development was not altered by acaricide treatments; (3) coumaphos exposure decreased lysozyme expression; (4) N. ceranae reduced levels of vitellogenin transcripts independently of the presence of acaricides. However, combined effects among stressors on imagoes were not recorded. Sublethal doses of acaricides and their interaction with other ubiquitous parasites in colonies, extending the experimental time, are of particular interest in further research work.
Fil: Garrido, Paula Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; Argentina
Fil: Antúnez, karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Branchiccela, Belén. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina
Fil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Salerno, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; Argentina
Fil: Ieno, Elena. Highland Statistics; España
Materia
APIS MELLIFERA
ACARICIDES
NOSEMA CERANAE
SUBLETHAL DOSES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22760
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22760
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybeesGarrido, Paula MelisaPorrini, Martín PabloAntúnez, karinaBranchiccela, BelénMartínez Noël, Giselle María AstridZunino, PabloSalerno, Graciela LidiaEguaras, Martin JavierIeno, ElenaAPIS MELLIFERAACARICIDESNOSEMA CERANAESUBLETHAL DOSEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control Varroa mites. These compounds can accumulate at high concentrations in apicultural matrices. In this work, the effects of parasitosis/acaricide on genes involved in honeybee immunity and survival were evaluated. Nurse bees were infected with N. ceranae and/or were chronically treated with sublethal doses of coumaphos or tau-fluvalinate, the two most abundant pesticides recorded in productive hives. Our results demonstrate the following: (1) honeybee survival was not affected by any of the treatments; (2) parasite development was not altered by acaricide treatments; (3) coumaphos exposure decreased lysozyme expression; (4) N. ceranae reduced levels of vitellogenin transcripts independently of the presence of acaricides. However, combined effects among stressors on imagoes were not recorded. Sublethal doses of acaricides and their interaction with other ubiquitous parasites in colonies, extending the experimental time, are of particular interest in further research work.Fil: Garrido, Paula Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; ArgentinaFil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; ArgentinaFil: Antúnez, karina. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Branchiccela, Belén. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; ArgentinaFil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Salerno, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; ArgentinaFil: Ieno, Elena. Highland Statistics; EspañaEDP Sciences2016-04-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22760Garrido, Paula Melisa; Porrini, Martín Pablo; Antúnez, karina; Branchiccela, Belén; Martínez Noël, Giselle María Astrid; et al.; Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees; EDP Sciences; Veterinary Research; 47; 51; 26-4-2016; 51-600928-42491297-9716CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-016-0335-zinfo:eu-repo/semantics/altIdentifier/url/https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-016-0335-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:47Zoai:ri.conicet.gov.ar:11336/22760instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:47.32CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
title Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
spellingShingle Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
Garrido, Paula Melisa
APIS MELLIFERA
ACARICIDES
NOSEMA CERANAE
SUBLETHAL DOSES
title_short Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
title_full Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
title_fullStr Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
title_full_unstemmed Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
title_sort Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees
dc.creator.none.fl_str_mv Garrido, Paula Melisa
Porrini, Martín Pablo
Antúnez, karina
Branchiccela, Belén
Martínez Noël, Giselle María Astrid
Zunino, Pablo
Salerno, Graciela Lidia
Eguaras, Martin Javier
Ieno, Elena
author Garrido, Paula Melisa
author_facet Garrido, Paula Melisa
Porrini, Martín Pablo
Antúnez, karina
Branchiccela, Belén
Martínez Noël, Giselle María Astrid
Zunino, Pablo
Salerno, Graciela Lidia
Eguaras, Martin Javier
Ieno, Elena
author_role author
author2 Porrini, Martín Pablo
Antúnez, karina
Branchiccela, Belén
Martínez Noël, Giselle María Astrid
Zunino, Pablo
Salerno, Graciela Lidia
Eguaras, Martin Javier
Ieno, Elena
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APIS MELLIFERA
ACARICIDES
NOSEMA CERANAE
SUBLETHAL DOSES
topic APIS MELLIFERA
ACARICIDES
NOSEMA CERANAE
SUBLETHAL DOSES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control Varroa mites. These compounds can accumulate at high concentrations in apicultural matrices. In this work, the effects of parasitosis/acaricide on genes involved in honeybee immunity and survival were evaluated. Nurse bees were infected with N. ceranae and/or were chronically treated with sublethal doses of coumaphos or tau-fluvalinate, the two most abundant pesticides recorded in productive hives. Our results demonstrate the following: (1) honeybee survival was not affected by any of the treatments; (2) parasite development was not altered by acaricide treatments; (3) coumaphos exposure decreased lysozyme expression; (4) N. ceranae reduced levels of vitellogenin transcripts independently of the presence of acaricides. However, combined effects among stressors on imagoes were not recorded. Sublethal doses of acaricides and their interaction with other ubiquitous parasites in colonies, extending the experimental time, are of particular interest in further research work.
Fil: Garrido, Paula Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; Argentina
Fil: Antúnez, karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Branchiccela, Belén. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina
Fil: Zunino, Pablo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Salerno, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Cs. Exactas y Naturales. Centro de Investigación en Abejas Sociales; Argentina
Fil: Ieno, Elena. Highland Statistics; España
description Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control Varroa mites. These compounds can accumulate at high concentrations in apicultural matrices. In this work, the effects of parasitosis/acaricide on genes involved in honeybee immunity and survival were evaluated. Nurse bees were infected with N. ceranae and/or were chronically treated with sublethal doses of coumaphos or tau-fluvalinate, the two most abundant pesticides recorded in productive hives. Our results demonstrate the following: (1) honeybee survival was not affected by any of the treatments; (2) parasite development was not altered by acaricide treatments; (3) coumaphos exposure decreased lysozyme expression; (4) N. ceranae reduced levels of vitellogenin transcripts independently of the presence of acaricides. However, combined effects among stressors on imagoes were not recorded. Sublethal doses of acaricides and their interaction with other ubiquitous parasites in colonies, extending the experimental time, are of particular interest in further research work.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22760
Garrido, Paula Melisa; Porrini, Martín Pablo; Antúnez, karina; Branchiccela, Belén; Martínez Noël, Giselle María Astrid; et al.; Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees; EDP Sciences; Veterinary Research; 47; 51; 26-4-2016; 51-60
0928-4249
1297-9716
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22760
identifier_str_mv Garrido, Paula Melisa; Porrini, Martín Pablo; Antúnez, karina; Branchiccela, Belén; Martínez Noël, Giselle María Astrid; et al.; Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees; EDP Sciences; Veterinary Research; 47; 51; 26-4-2016; 51-60
0928-4249
1297-9716
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-016-0335-z
info:eu-repo/semantics/altIdentifier/url/https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-016-0335-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv EDP Sciences
publisher.none.fl_str_mv EDP Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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