Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation

Autores
Suarez, María Fernanda; Echenique, Jose Ricardo; Lopez, Juan Manuel; Medina, Esteban Roberto; Irós, Mariano; Serra, Horacio Marcelo; Fini, M. Elizabeth
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Solar damage due to ultraviolet radiation (UVR) is implicated in the development of two proliferative lesions of the ocular surface: pterygium and pinguecula. Pterygium and pinguecula specimens were collected, along with adjacent healthy conjunctiva specimens. RNA was extracted and sequenced. Pairwise comparisons were made of differentially expressed genes (DEGs). Computational methods were used for analysis. Transcripts from 18,630 genes were identified. Comparison of two subgroups of pterygium specimens uncovered evidence of genomic instability associated with inflammation and the immune response; these changes were also observed in pinguecula, but to a lesser extent. Among the top DEGs were four genes encoding tumor suppressors that were downregulated in pterygium: C10orf90, RARRES1, DMBT1 and SCGB3A1; C10orf90 and RARRES1 were also downregulated in pinguecula. Ingenuity Pathway Analysis overwhelmingly linked DEGs to cancer for both lesions; however, both lesions are clearly still benign, as evidenced by the expression of other genes indicating their well‐differentiated and non‐invasive character. Pathways for epithelial cell proliferation were identified that distinguish the two lesions, as well as genes encoding specific pathway components. Upregulated DEGs common to both lesions, including KRT9 and TRPV3, provide a further insight into pathophysiology. Our findings suggest that pterygium and pinguecula, while benign lesions, are both on the pathological pathway towards neoplastic trans-formation.
Fil: Suarez, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Tufts University School of Medicine; Estados Unidos
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lopez, Juan Manuel. Instituto de Microcirugía Ocular Córdoba; Argentina
Fil: Medina, Esteban Roberto. Instituto de Microcirugia Ocular Cordoba; Argentina
Fil: Irós, Mariano. Instituto de Microcirugia Ocular Cordoba; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Fini, M. Elizabeth. Tufts Graduate School Of Biomedical Sciences, Boston; Estados Unidos
Materia
CANCER
CONJUNCTIVA
GENOMIC INSTABILITY
IMMUNE RESPONSE
INFLAMMATION
OCULAR SURFACE EPITHELIA
PINGUECULA
PTERYGIUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184735
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammationSuarez, María FernandaEchenique, Jose RicardoLopez, Juan ManuelMedina, Esteban RobertoIrós, MarianoSerra, Horacio MarceloFini, M. ElizabethCANCERCONJUNCTIVAGENOMIC INSTABILITYIMMUNE RESPONSEINFLAMMATIONOCULAR SURFACE EPITHELIAPINGUECULAPTERYGIUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Solar damage due to ultraviolet radiation (UVR) is implicated in the development of two proliferative lesions of the ocular surface: pterygium and pinguecula. Pterygium and pinguecula specimens were collected, along with adjacent healthy conjunctiva specimens. RNA was extracted and sequenced. Pairwise comparisons were made of differentially expressed genes (DEGs). Computational methods were used for analysis. Transcripts from 18,630 genes were identified. Comparison of two subgroups of pterygium specimens uncovered evidence of genomic instability associated with inflammation and the immune response; these changes were also observed in pinguecula, but to a lesser extent. Among the top DEGs were four genes encoding tumor suppressors that were downregulated in pterygium: C10orf90, RARRES1, DMBT1 and SCGB3A1; C10orf90 and RARRES1 were also downregulated in pinguecula. Ingenuity Pathway Analysis overwhelmingly linked DEGs to cancer for both lesions; however, both lesions are clearly still benign, as evidenced by the expression of other genes indicating their well‐differentiated and non‐invasive character. Pathways for epithelial cell proliferation were identified that distinguish the two lesions, as well as genes encoding specific pathway components. Upregulated DEGs common to both lesions, including KRT9 and TRPV3, provide a further insight into pathophysiology. Our findings suggest that pterygium and pinguecula, while benign lesions, are both on the pathological pathway towards neoplastic trans-formation.Fil: Suarez, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Tufts University School of Medicine; Estados UnidosFil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Lopez, Juan Manuel. Instituto de Microcirugía Ocular Córdoba; ArgentinaFil: Medina, Esteban Roberto. Instituto de Microcirugia Ocular Cordoba; ArgentinaFil: Irós, Mariano. Instituto de Microcirugia Ocular Cordoba; ArgentinaFil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Fini, M. Elizabeth. Tufts Graduate School Of Biomedical Sciences, Boston; Estados UnidosMDPI2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184735Suarez, María Fernanda; Echenique, Jose Ricardo; Lopez, Juan Manuel; Medina, Esteban Roberto; Irós, Mariano; et al.; Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation; MDPI; International Journal of Molecular Sciences; 22; 21; 11-2021; 1-291422-00671422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/ijms222112090info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/21/12090info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:03:41Zoai:ri.conicet.gov.ar:11336/184735instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:03:41.573CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
title Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
spellingShingle Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
Suarez, María Fernanda
CANCER
CONJUNCTIVA
GENOMIC INSTABILITY
IMMUNE RESPONSE
INFLAMMATION
OCULAR SURFACE EPITHELIA
PINGUECULA
PTERYGIUM
title_short Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
title_full Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
title_fullStr Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
title_full_unstemmed Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
title_sort Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation
dc.creator.none.fl_str_mv Suarez, María Fernanda
Echenique, Jose Ricardo
Lopez, Juan Manuel
Medina, Esteban Roberto
Irós, Mariano
Serra, Horacio Marcelo
Fini, M. Elizabeth
author Suarez, María Fernanda
author_facet Suarez, María Fernanda
Echenique, Jose Ricardo
Lopez, Juan Manuel
Medina, Esteban Roberto
Irós, Mariano
Serra, Horacio Marcelo
Fini, M. Elizabeth
author_role author
author2 Echenique, Jose Ricardo
Lopez, Juan Manuel
Medina, Esteban Roberto
Irós, Mariano
Serra, Horacio Marcelo
Fini, M. Elizabeth
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CANCER
CONJUNCTIVA
GENOMIC INSTABILITY
IMMUNE RESPONSE
INFLAMMATION
OCULAR SURFACE EPITHELIA
PINGUECULA
PTERYGIUM
topic CANCER
CONJUNCTIVA
GENOMIC INSTABILITY
IMMUNE RESPONSE
INFLAMMATION
OCULAR SURFACE EPITHELIA
PINGUECULA
PTERYGIUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Solar damage due to ultraviolet radiation (UVR) is implicated in the development of two proliferative lesions of the ocular surface: pterygium and pinguecula. Pterygium and pinguecula specimens were collected, along with adjacent healthy conjunctiva specimens. RNA was extracted and sequenced. Pairwise comparisons were made of differentially expressed genes (DEGs). Computational methods were used for analysis. Transcripts from 18,630 genes were identified. Comparison of two subgroups of pterygium specimens uncovered evidence of genomic instability associated with inflammation and the immune response; these changes were also observed in pinguecula, but to a lesser extent. Among the top DEGs were four genes encoding tumor suppressors that were downregulated in pterygium: C10orf90, RARRES1, DMBT1 and SCGB3A1; C10orf90 and RARRES1 were also downregulated in pinguecula. Ingenuity Pathway Analysis overwhelmingly linked DEGs to cancer for both lesions; however, both lesions are clearly still benign, as evidenced by the expression of other genes indicating their well‐differentiated and non‐invasive character. Pathways for epithelial cell proliferation were identified that distinguish the two lesions, as well as genes encoding specific pathway components. Upregulated DEGs common to both lesions, including KRT9 and TRPV3, provide a further insight into pathophysiology. Our findings suggest that pterygium and pinguecula, while benign lesions, are both on the pathological pathway towards neoplastic trans-formation.
Fil: Suarez, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Tufts University School of Medicine; Estados Unidos
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lopez, Juan Manuel. Instituto de Microcirugía Ocular Córdoba; Argentina
Fil: Medina, Esteban Roberto. Instituto de Microcirugia Ocular Cordoba; Argentina
Fil: Irós, Mariano. Instituto de Microcirugia Ocular Cordoba; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Fini, M. Elizabeth. Tufts Graduate School Of Biomedical Sciences, Boston; Estados Unidos
description Solar damage due to ultraviolet radiation (UVR) is implicated in the development of two proliferative lesions of the ocular surface: pterygium and pinguecula. Pterygium and pinguecula specimens were collected, along with adjacent healthy conjunctiva specimens. RNA was extracted and sequenced. Pairwise comparisons were made of differentially expressed genes (DEGs). Computational methods were used for analysis. Transcripts from 18,630 genes were identified. Comparison of two subgroups of pterygium specimens uncovered evidence of genomic instability associated with inflammation and the immune response; these changes were also observed in pinguecula, but to a lesser extent. Among the top DEGs were four genes encoding tumor suppressors that were downregulated in pterygium: C10orf90, RARRES1, DMBT1 and SCGB3A1; C10orf90 and RARRES1 were also downregulated in pinguecula. Ingenuity Pathway Analysis overwhelmingly linked DEGs to cancer for both lesions; however, both lesions are clearly still benign, as evidenced by the expression of other genes indicating their well‐differentiated and non‐invasive character. Pathways for epithelial cell proliferation were identified that distinguish the two lesions, as well as genes encoding specific pathway components. Upregulated DEGs common to both lesions, including KRT9 and TRPV3, provide a further insight into pathophysiology. Our findings suggest that pterygium and pinguecula, while benign lesions, are both on the pathological pathway towards neoplastic trans-formation.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184735
Suarez, María Fernanda; Echenique, Jose Ricardo; Lopez, Juan Manuel; Medina, Esteban Roberto; Irós, Mariano; et al.; Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation; MDPI; International Journal of Molecular Sciences; 22; 21; 11-2021; 1-29
1422-0067
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184735
identifier_str_mv Suarez, María Fernanda; Echenique, Jose Ricardo; Lopez, Juan Manuel; Medina, Esteban Roberto; Irós, Mariano; et al.; Transcriptome analysis of pterygium and pinguecula reveals evidence of genomic instability associated with chronic inflammation; MDPI; International Journal of Molecular Sciences; 22; 21; 11-2021; 1-29
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms222112090
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/21/12090
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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