Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)

Autores
Tomas, Pablo Andrés; González, Graciela Esther; Schrauf, Gustavo Enrique; Poggio, Lidia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Karyotype of Elymus scabrifolius (2n=4x=28) was investigated by DAPI staining and Fluorescent In Situ Hybridization (FISH). All the accessions studied presented a symmetric and uniform karyotype constituted by 9 m + 3 m-sm + 2 sm. DAPI stain showed 1 to 7 conspicuous bands in all the chromosomes and polymorphisms between accessions were detected. FISH experiments carried out with the 45S rDNA probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair No. 8; the submetacentric SAT-chromosome pair No. 13 presented weaker hybridization. FISH using pSc119.2 clone as probe let to identify five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. GISH (Genomic In Situ Hybridization) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, and enabled to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA probe let to assign the genomic provenance of most of chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome.
Fil: Tomas, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: González, Graciela Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Schrauf, Gustavo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
ELYMUS SCABRIFOLIUS
KARYOTYPE
FISH, GISH
INTERGENOMIC REARRANGEMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272302
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272302
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)Tomas, Pablo AndrésGonzález, Graciela EstherSchrauf, Gustavo EnriquePoggio, LidiaELYMUS SCABRIFOLIUSKARYOTYPEFISH, GISHINTERGENOMIC REARRANGEMENThttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Karyotype of Elymus scabrifolius (2n=4x=28) was investigated by DAPI staining and Fluorescent In Situ Hybridization (FISH). All the accessions studied presented a symmetric and uniform karyotype constituted by 9 m + 3 m-sm + 2 sm. DAPI stain showed 1 to 7 conspicuous bands in all the chromosomes and polymorphisms between accessions were detected. FISH experiments carried out with the 45S rDNA probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair No. 8; the submetacentric SAT-chromosome pair No. 13 presented weaker hybridization. FISH using pSc119.2 clone as probe let to identify five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. GISH (Genomic In Situ Hybridization) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, and enabled to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA probe let to assign the genomic provenance of most of chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome.Fil: Tomas, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: González, Graciela Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Schrauf, Gustavo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaNational Research Council Canada-NRC Research Press2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272302Tomas, Pablo Andrés; González, Graciela Esther; Schrauf, Gustavo Enrique; Poggio, Lidia; Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH); National Research Council Canada-NRC Research Press; Genome; 55; 8; 7-2012; 591-5980831-2796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://cdnsciencepub.com/doi/10.1139/g2012-046info:eu-repo/semantics/altIdentifier/doi/10.1139/g2012-046info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:34Zoai:ri.conicet.gov.ar:11336/272302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:34.768CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
title Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
spellingShingle Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
Tomas, Pablo Andrés
ELYMUS SCABRIFOLIUS
KARYOTYPE
FISH, GISH
INTERGENOMIC REARRANGEMENT
title_short Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
title_full Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
title_fullStr Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
title_full_unstemmed Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
title_sort Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)
dc.creator.none.fl_str_mv Tomas, Pablo Andrés
González, Graciela Esther
Schrauf, Gustavo Enrique
Poggio, Lidia
author Tomas, Pablo Andrés
author_facet Tomas, Pablo Andrés
González, Graciela Esther
Schrauf, Gustavo Enrique
Poggio, Lidia
author_role author
author2 González, Graciela Esther
Schrauf, Gustavo Enrique
Poggio, Lidia
author2_role author
author
author
dc.subject.none.fl_str_mv ELYMUS SCABRIFOLIUS
KARYOTYPE
FISH, GISH
INTERGENOMIC REARRANGEMENT
topic ELYMUS SCABRIFOLIUS
KARYOTYPE
FISH, GISH
INTERGENOMIC REARRANGEMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Karyotype of Elymus scabrifolius (2n=4x=28) was investigated by DAPI staining and Fluorescent In Situ Hybridization (FISH). All the accessions studied presented a symmetric and uniform karyotype constituted by 9 m + 3 m-sm + 2 sm. DAPI stain showed 1 to 7 conspicuous bands in all the chromosomes and polymorphisms between accessions were detected. FISH experiments carried out with the 45S rDNA probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair No. 8; the submetacentric SAT-chromosome pair No. 13 presented weaker hybridization. FISH using pSc119.2 clone as probe let to identify five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. GISH (Genomic In Situ Hybridization) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, and enabled to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA probe let to assign the genomic provenance of most of chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome.
Fil: Tomas, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: González, Graciela Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Schrauf, Gustavo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description Karyotype of Elymus scabrifolius (2n=4x=28) was investigated by DAPI staining and Fluorescent In Situ Hybridization (FISH). All the accessions studied presented a symmetric and uniform karyotype constituted by 9 m + 3 m-sm + 2 sm. DAPI stain showed 1 to 7 conspicuous bands in all the chromosomes and polymorphisms between accessions were detected. FISH experiments carried out with the 45S rDNA probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair No. 8; the submetacentric SAT-chromosome pair No. 13 presented weaker hybridization. FISH using pSc119.2 clone as probe let to identify five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. GISH (Genomic In Situ Hybridization) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, and enabled to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA probe let to assign the genomic provenance of most of chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272302
Tomas, Pablo Andrés; González, Graciela Esther; Schrauf, Gustavo Enrique; Poggio, Lidia; Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH); National Research Council Canada-NRC Research Press; Genome; 55; 8; 7-2012; 591-598
0831-2796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272302
identifier_str_mv Tomas, Pablo Andrés; González, Graciela Esther; Schrauf, Gustavo Enrique; Poggio, Lidia; Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH); National Research Council Canada-NRC Research Press; Genome; 55; 8; 7-2012; 591-598
0831-2796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://cdnsciencepub.com/doi/10.1139/g2012-046
info:eu-repo/semantics/altIdentifier/doi/10.1139/g2012-046
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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