Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH

Autores
Silva, Gonzalo Santos; Souza, Margarete; de Melo, Cláusio Antonio; Urdampilleta, Juan Domingo; Forni-Martins, Eliana Regina
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A great interest exists in the production of hybrid plants of the genus Passiflora given the beauty and exotic features of its flowers which have ornamental value. Hybrid paternity confirmation is therefore important for assuring germplasm origin, and is typically carried out by molecular marker segregation. The aim of this study was to karyotypically characterize the chromosome heritance patterns of the progeny resultant from a cross of P. gardneri and P. gibertii using classical cytogenetics, chromosome banding, and molecular cytogenetics.ResultsAll analyzed genotypes showed the same diploid chromosome number as the genitor species: 2n = 18. Classical and CMA3 and DAPI staining allowed for chromosome counting and satellite identification (secondary constrictions). Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used to characterize subgenomes by either identifying rDNA-specific genome patterns or parental genomes, respectively.ConclusionsThe heritance of chromosomal markers presenting rDNA sites from each parent for genome identification confirmed that all obtained plants were hybrids. These results will improve breeding programs involving the species of this genus. Apart from confirming hybridization, GISH allowed the visualization of recombination between the homeologous chromosome and the introgression of sequences of interest.
Fil: Silva, Gonzalo Santos. Universidade Estadual de Santa Cruz; Brasil
Fil: Souza, Margarete. Universidade Estadual de Santa Cruz; Brasil
Fil: de Melo, Cláusio Antonio. Universidade Estadual de Santa Cruz; Brasil
Fil: Urdampilleta, Juan Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Forni-Martins, Eliana Regina. Universidade Estadual de Campinas; Brasil
Materia
CMA3 AND DAPI BANDING
FISH
GISH
INTERSPECIFIC HYBRIDS
PASSION FLOWERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/86546
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification and characterization of karyotype in Passiflora hybrids using FISH and GISHSilva, Gonzalo SantosSouza, Margaretede Melo, Cláusio AntonioUrdampilleta, Juan DomingoForni-Martins, Eliana ReginaCMA3 AND DAPI BANDINGFISHGISHINTERSPECIFIC HYBRIDSPASSION FLOWERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A great interest exists in the production of hybrid plants of the genus Passiflora given the beauty and exotic features of its flowers which have ornamental value. Hybrid paternity confirmation is therefore important for assuring germplasm origin, and is typically carried out by molecular marker segregation. The aim of this study was to karyotypically characterize the chromosome heritance patterns of the progeny resultant from a cross of P. gardneri and P. gibertii using classical cytogenetics, chromosome banding, and molecular cytogenetics.ResultsAll analyzed genotypes showed the same diploid chromosome number as the genitor species: 2n = 18. Classical and CMA3 and DAPI staining allowed for chromosome counting and satellite identification (secondary constrictions). Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used to characterize subgenomes by either identifying rDNA-specific genome patterns or parental genomes, respectively.ConclusionsThe heritance of chromosomal markers presenting rDNA sites from each parent for genome identification confirmed that all obtained plants were hybrids. These results will improve breeding programs involving the species of this genus. Apart from confirming hybridization, GISH allowed the visualization of recombination between the homeologous chromosome and the introgression of sequences of interest.Fil: Silva, Gonzalo Santos. Universidade Estadual de Santa Cruz; BrasilFil: Souza, Margarete. Universidade Estadual de Santa Cruz; BrasilFil: de Melo, Cláusio Antonio. Universidade Estadual de Santa Cruz; BrasilFil: Urdampilleta, Juan Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Forni-Martins, Eliana Regina. Universidade Estadual de Campinas; BrasilBioMed Central2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86546Silva, Gonzalo Santos; Souza, Margarete ; de Melo, Cláusio Antonio; Urdampilleta, Juan Domingo; Forni-Martins, Eliana Regina; Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH; BioMed Central; BMC Genetics; 19; 1; 4-20181471-2156CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-018-0612-0info:eu-repo/semantics/altIdentifier/doi/10.1186/s12863-018-0612-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:20Zoai:ri.conicet.gov.ar:11336/86546instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:20.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
title Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
spellingShingle Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
Silva, Gonzalo Santos
CMA3 AND DAPI BANDING
FISH
GISH
INTERSPECIFIC HYBRIDS
PASSION FLOWERS
title_short Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
title_full Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
title_fullStr Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
title_full_unstemmed Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
title_sort Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH
dc.creator.none.fl_str_mv Silva, Gonzalo Santos
Souza, Margarete
de Melo, Cláusio Antonio
Urdampilleta, Juan Domingo
Forni-Martins, Eliana Regina
author Silva, Gonzalo Santos
author_facet Silva, Gonzalo Santos
Souza, Margarete
de Melo, Cláusio Antonio
Urdampilleta, Juan Domingo
Forni-Martins, Eliana Regina
author_role author
author2 Souza, Margarete
de Melo, Cláusio Antonio
Urdampilleta, Juan Domingo
Forni-Martins, Eliana Regina
author2_role author
author
author
author
dc.subject.none.fl_str_mv CMA3 AND DAPI BANDING
FISH
GISH
INTERSPECIFIC HYBRIDS
PASSION FLOWERS
topic CMA3 AND DAPI BANDING
FISH
GISH
INTERSPECIFIC HYBRIDS
PASSION FLOWERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A great interest exists in the production of hybrid plants of the genus Passiflora given the beauty and exotic features of its flowers which have ornamental value. Hybrid paternity confirmation is therefore important for assuring germplasm origin, and is typically carried out by molecular marker segregation. The aim of this study was to karyotypically characterize the chromosome heritance patterns of the progeny resultant from a cross of P. gardneri and P. gibertii using classical cytogenetics, chromosome banding, and molecular cytogenetics.ResultsAll analyzed genotypes showed the same diploid chromosome number as the genitor species: 2n = 18. Classical and CMA3 and DAPI staining allowed for chromosome counting and satellite identification (secondary constrictions). Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used to characterize subgenomes by either identifying rDNA-specific genome patterns or parental genomes, respectively.ConclusionsThe heritance of chromosomal markers presenting rDNA sites from each parent for genome identification confirmed that all obtained plants were hybrids. These results will improve breeding programs involving the species of this genus. Apart from confirming hybridization, GISH allowed the visualization of recombination between the homeologous chromosome and the introgression of sequences of interest.
Fil: Silva, Gonzalo Santos. Universidade Estadual de Santa Cruz; Brasil
Fil: Souza, Margarete. Universidade Estadual de Santa Cruz; Brasil
Fil: de Melo, Cláusio Antonio. Universidade Estadual de Santa Cruz; Brasil
Fil: Urdampilleta, Juan Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Forni-Martins, Eliana Regina. Universidade Estadual de Campinas; Brasil
description A great interest exists in the production of hybrid plants of the genus Passiflora given the beauty and exotic features of its flowers which have ornamental value. Hybrid paternity confirmation is therefore important for assuring germplasm origin, and is typically carried out by molecular marker segregation. The aim of this study was to karyotypically characterize the chromosome heritance patterns of the progeny resultant from a cross of P. gardneri and P. gibertii using classical cytogenetics, chromosome banding, and molecular cytogenetics.ResultsAll analyzed genotypes showed the same diploid chromosome number as the genitor species: 2n = 18. Classical and CMA3 and DAPI staining allowed for chromosome counting and satellite identification (secondary constrictions). Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used to characterize subgenomes by either identifying rDNA-specific genome patterns or parental genomes, respectively.ConclusionsThe heritance of chromosomal markers presenting rDNA sites from each parent for genome identification confirmed that all obtained plants were hybrids. These results will improve breeding programs involving the species of this genus. Apart from confirming hybridization, GISH allowed the visualization of recombination between the homeologous chromosome and the introgression of sequences of interest.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86546
Silva, Gonzalo Santos; Souza, Margarete ; de Melo, Cláusio Antonio; Urdampilleta, Juan Domingo; Forni-Martins, Eliana Regina; Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH; BioMed Central; BMC Genetics; 19; 1; 4-2018
1471-2156
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86546
identifier_str_mv Silva, Gonzalo Santos; Souza, Margarete ; de Melo, Cláusio Antonio; Urdampilleta, Juan Domingo; Forni-Martins, Eliana Regina; Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH; BioMed Central; BMC Genetics; 19; 1; 4-2018
1471-2156
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-018-0612-0
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12863-018-0612-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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