Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2

Autores
Roberts, Robyn; Liu, Alexander E.; Wan, Lingwei; Geiger, Annie M.; Hind, Sarah R.; Rosli, Hernan Guillermo; Martin, Gregory B.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plants mount defense responses by recognizing indicators of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin, from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst), contains two MAMPs, flg22 and flgII-28, that are recognized by tomato (Solanum lycopersicum) receptors Flagellin sensing2 (Fls2) and Fls3, respectively, but to what degree each receptor contributes to immunity and whether they promote immune responses using the same molecular mechanisms are unknown. Here, we characterized CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants and found that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, we observed striking differences in certain host responses mediated by the two receptors. Compared to Fls2, Fls3 mediated a more sustained production of reactive oxygen species and an increase in transcript abundance of 44 tomato genes, with two genes serving as specific reporters for the Fls3 pathway. Fls3 had greater in vitro kinase activity than Fls2 and could transphosphorylate a substrate. Using chimeric Fls2/Fls3 proteins, we found no evidence that a single receptor domain is responsible for the Fls3-sustained reactive oxygen species, suggesting involvement of multiple structural features or a nullified function of the chimeric construct. This work reveals differences in certain immunity outputs between Fls2 and Fls3, suggesting that they might use distinct molecular mechanisms to activate pattern-triggered immunity in response to flagellin-derived MAMPs.
Fil: Roberts, Robyn. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Liu, Alexander E.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Wan, Lingwei. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Geiger, Annie M.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Hind, Sarah R.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Materia
PLANT IMMUNITY
FLAGELLIN
PRR-TRIGGERED IMMUNITY
BACTERIAL SPECK DISEASE
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181156
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181156
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2Roberts, RobynLiu, Alexander E.Wan, LingweiGeiger, Annie M.Hind, Sarah R.Rosli, Hernan GuillermoMartin, Gregory B.PLANT IMMUNITYFLAGELLINPRR-TRIGGERED IMMUNITYBACTERIAL SPECK DISEASETOMATOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plants mount defense responses by recognizing indicators of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin, from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst), contains two MAMPs, flg22 and flgII-28, that are recognized by tomato (Solanum lycopersicum) receptors Flagellin sensing2 (Fls2) and Fls3, respectively, but to what degree each receptor contributes to immunity and whether they promote immune responses using the same molecular mechanisms are unknown. Here, we characterized CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants and found that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, we observed striking differences in certain host responses mediated by the two receptors. Compared to Fls2, Fls3 mediated a more sustained production of reactive oxygen species and an increase in transcript abundance of 44 tomato genes, with two genes serving as specific reporters for the Fls3 pathway. Fls3 had greater in vitro kinase activity than Fls2 and could transphosphorylate a substrate. Using chimeric Fls2/Fls3 proteins, we found no evidence that a single receptor domain is responsible for the Fls3-sustained reactive oxygen species, suggesting involvement of multiple structural features or a nullified function of the chimeric construct. This work reveals differences in certain immunity outputs between Fls2 and Fls3, suggesting that they might use distinct molecular mechanisms to activate pattern-triggered immunity in response to flagellin-derived MAMPs.Fil: Roberts, Robyn. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Liu, Alexander E.. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Wan, Lingwei. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Geiger, Annie M.. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Hind, Sarah R.. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados UnidosAmerican Society of Plant Biologist2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181156Roberts, Robyn; Liu, Alexander E.; Wan, Lingwei; Geiger, Annie M.; Hind, Sarah R.; et al.; Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2; American Society of Plant Biologist; Plant Physiology; 183; 4; 8-2020; 1825-18370032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.20.00184info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/183/4/1825/6118505info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401135/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:18Zoai:ri.conicet.gov.ar:11336/181156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:18.805CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
title Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
spellingShingle Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
Roberts, Robyn
PLANT IMMUNITY
FLAGELLIN
PRR-TRIGGERED IMMUNITY
BACTERIAL SPECK DISEASE
TOMATO
title_short Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
title_full Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
title_fullStr Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
title_full_unstemmed Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
title_sort Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2
dc.creator.none.fl_str_mv Roberts, Robyn
Liu, Alexander E.
Wan, Lingwei
Geiger, Annie M.
Hind, Sarah R.
Rosli, Hernan Guillermo
Martin, Gregory B.
author Roberts, Robyn
author_facet Roberts, Robyn
Liu, Alexander E.
Wan, Lingwei
Geiger, Annie M.
Hind, Sarah R.
Rosli, Hernan Guillermo
Martin, Gregory B.
author_role author
author2 Liu, Alexander E.
Wan, Lingwei
Geiger, Annie M.
Hind, Sarah R.
Rosli, Hernan Guillermo
Martin, Gregory B.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANT IMMUNITY
FLAGELLIN
PRR-TRIGGERED IMMUNITY
BACTERIAL SPECK DISEASE
TOMATO
topic PLANT IMMUNITY
FLAGELLIN
PRR-TRIGGERED IMMUNITY
BACTERIAL SPECK DISEASE
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plants mount defense responses by recognizing indicators of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin, from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst), contains two MAMPs, flg22 and flgII-28, that are recognized by tomato (Solanum lycopersicum) receptors Flagellin sensing2 (Fls2) and Fls3, respectively, but to what degree each receptor contributes to immunity and whether they promote immune responses using the same molecular mechanisms are unknown. Here, we characterized CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants and found that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, we observed striking differences in certain host responses mediated by the two receptors. Compared to Fls2, Fls3 mediated a more sustained production of reactive oxygen species and an increase in transcript abundance of 44 tomato genes, with two genes serving as specific reporters for the Fls3 pathway. Fls3 had greater in vitro kinase activity than Fls2 and could transphosphorylate a substrate. Using chimeric Fls2/Fls3 proteins, we found no evidence that a single receptor domain is responsible for the Fls3-sustained reactive oxygen species, suggesting involvement of multiple structural features or a nullified function of the chimeric construct. This work reveals differences in certain immunity outputs between Fls2 and Fls3, suggesting that they might use distinct molecular mechanisms to activate pattern-triggered immunity in response to flagellin-derived MAMPs.
Fil: Roberts, Robyn. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Liu, Alexander E.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Wan, Lingwei. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Geiger, Annie M.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Hind, Sarah R.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
description Plants mount defense responses by recognizing indicators of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin, from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst), contains two MAMPs, flg22 and flgII-28, that are recognized by tomato (Solanum lycopersicum) receptors Flagellin sensing2 (Fls2) and Fls3, respectively, but to what degree each receptor contributes to immunity and whether they promote immune responses using the same molecular mechanisms are unknown. Here, we characterized CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants and found that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, we observed striking differences in certain host responses mediated by the two receptors. Compared to Fls2, Fls3 mediated a more sustained production of reactive oxygen species and an increase in transcript abundance of 44 tomato genes, with two genes serving as specific reporters for the Fls3 pathway. Fls3 had greater in vitro kinase activity than Fls2 and could transphosphorylate a substrate. Using chimeric Fls2/Fls3 proteins, we found no evidence that a single receptor domain is responsible for the Fls3-sustained reactive oxygen species, suggesting involvement of multiple structural features or a nullified function of the chimeric construct. This work reveals differences in certain immunity outputs between Fls2 and Fls3, suggesting that they might use distinct molecular mechanisms to activate pattern-triggered immunity in response to flagellin-derived MAMPs.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181156
Roberts, Robyn; Liu, Alexander E.; Wan, Lingwei; Geiger, Annie M.; Hind, Sarah R.; et al.; Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2; American Society of Plant Biologist; Plant Physiology; 183; 4; 8-2020; 1825-1837
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181156
identifier_str_mv Roberts, Robyn; Liu, Alexander E.; Wan, Lingwei; Geiger, Annie M.; Hind, Sarah R.; et al.; Molecular characterization of differences between the tomato immune receptors flagellin sensing 3 and flagellin sensing 2; American Society of Plant Biologist; Plant Physiology; 183; 4; 8-2020; 1825-1837
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.20.00184
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/183/4/1825/6118505
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401135/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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