The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection

Autores
Schwizer, Simon; Kraus, Christine M.; Dunham, Diane M.; Zheng, Yi; Fernandez Pozo, Noé; Pombo, Marina Alejandra; Fei, Zhangjun; Chakravarthy, Suma; Martin, Gregory B.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against Pseudomonas syringae pv. tomato (Pst). The tomato genome has two Pti1 genes, referred to as Pti1a and Pti1b. A hairpin-Pti1 (hpPti1) construct was developed and was used to generate two independent stable transgenic tomato lines that had reduced transcript abundance of both genes. In response to P. syringae pv. tomato inoculation, these hpPti1 plants developed more severe disease symptoms, supported higher bacterial populations, and had reduced transcript accumulation of PTI-associated genes, as compared with wild-type plants. In response to two flagellin-derived peptides, the hpPti1 plants produced lesser amounts of reactive oxygen species (ROS) but showed no difference in mitogen-activated protein kinase (MAPK). Synthetic Pti1a and Pti1b genes designed to avoid silencing were transiently expressed in the hpPti1 plants and restored the ability of the plants to produce wild-type levels of ROS. Our results identify a new component of PTI in tomato that, because it affects ROS production but not MAPK signaling, appears to act early in the immune response.
Fil: Schwizer, Simon. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Fil: Kraus, Christine M.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Fil: Dunham, Diane M.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Zheng, Yi. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Fernandez Pozo, Noé. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Pombo, Marina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Fei, Zhangjun. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Fil: Chakravarthy, Suma. Cornell University; Estados Unidos
Fil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Materia
Pattern-triggered immunity
Pti1
ROS production
Tomato defense
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65416
Acceder
id CONICETDig_9791ee5a44b30e94c698c3ff2e377e66
oai_identifier_str oai:ri.conicet.gov.ar:11336/65416
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae InfectionSchwizer, SimonKraus, Christine M.Dunham, Diane M.Zheng, YiFernandez Pozo, NoéPombo, Marina AlejandraFei, ZhangjunChakravarthy, SumaMartin, Gregory B.Pattern-triggered immunityPti1ROS productionTomato defensehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against Pseudomonas syringae pv. tomato (Pst). The tomato genome has two Pti1 genes, referred to as Pti1a and Pti1b. A hairpin-Pti1 (hpPti1) construct was developed and was used to generate two independent stable transgenic tomato lines that had reduced transcript abundance of both genes. In response to P. syringae pv. tomato inoculation, these hpPti1 plants developed more severe disease symptoms, supported higher bacterial populations, and had reduced transcript accumulation of PTI-associated genes, as compared with wild-type plants. In response to two flagellin-derived peptides, the hpPti1 plants produced lesser amounts of reactive oxygen species (ROS) but showed no difference in mitogen-activated protein kinase (MAPK). Synthetic Pti1a and Pti1b genes designed to avoid silencing were transiently expressed in the hpPti1 plants and restored the ability of the plants to produce wild-type levels of ROS. Our results identify a new component of PTI in tomato that, because it affects ROS production but not MAPK signaling, appears to act early in the immune response.Fil: Schwizer, Simon. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados UnidosFil: Kraus, Christine M.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados UnidosFil: Dunham, Diane M.. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Zheng, Yi. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Fernandez Pozo, Noé. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Pombo, Marina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Boyce Thompson Institute for Plant Research; Estados UnidosFil: Fei, Zhangjun. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados UnidosFil: Chakravarthy, Suma. Cornell University; Estados UnidosFil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados UnidosAmerican Phytopathological Society2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/65416Schwizer, Simon; Kraus, Christine M.; Dunham, Diane M.; Zheng, Yi; Fernandez Pozo, Noé; et al.; The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 30; 9; 9-2017; 725-7380894-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-03-17-0056-Rinfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-17-0056-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:24Zoai:ri.conicet.gov.ar:11336/65416instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:25.256CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
spellingShingle The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
Schwizer, Simon
Pattern-triggered immunity
Pti1
ROS production
Tomato defense
title_short The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_full The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_fullStr The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_full_unstemmed The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_sort The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
dc.creator.none.fl_str_mv Schwizer, Simon
Kraus, Christine M.
Dunham, Diane M.
Zheng, Yi
Fernandez Pozo, Noé
Pombo, Marina Alejandra
Fei, Zhangjun
Chakravarthy, Suma
Martin, Gregory B.
author Schwizer, Simon
author_facet Schwizer, Simon
Kraus, Christine M.
Dunham, Diane M.
Zheng, Yi
Fernandez Pozo, Noé
Pombo, Marina Alejandra
Fei, Zhangjun
Chakravarthy, Suma
Martin, Gregory B.
author_role author
author2 Kraus, Christine M.
Dunham, Diane M.
Zheng, Yi
Fernandez Pozo, Noé
Pombo, Marina Alejandra
Fei, Zhangjun
Chakravarthy, Suma
Martin, Gregory B.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pattern-triggered immunity
Pti1
ROS production
Tomato defense
topic Pattern-triggered immunity
Pti1
ROS production
Tomato defense
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against Pseudomonas syringae pv. tomato (Pst). The tomato genome has two Pti1 genes, referred to as Pti1a and Pti1b. A hairpin-Pti1 (hpPti1) construct was developed and was used to generate two independent stable transgenic tomato lines that had reduced transcript abundance of both genes. In response to P. syringae pv. tomato inoculation, these hpPti1 plants developed more severe disease symptoms, supported higher bacterial populations, and had reduced transcript accumulation of PTI-associated genes, as compared with wild-type plants. In response to two flagellin-derived peptides, the hpPti1 plants produced lesser amounts of reactive oxygen species (ROS) but showed no difference in mitogen-activated protein kinase (MAPK). Synthetic Pti1a and Pti1b genes designed to avoid silencing were transiently expressed in the hpPti1 plants and restored the ability of the plants to produce wild-type levels of ROS. Our results identify a new component of PTI in tomato that, because it affects ROS production but not MAPK signaling, appears to act early in the immune response.
Fil: Schwizer, Simon. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Fil: Kraus, Christine M.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Fil: Dunham, Diane M.. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Zheng, Yi. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Fernandez Pozo, Noé. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Pombo, Marina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Boyce Thompson Institute for Plant Research; Estados Unidos
Fil: Fei, Zhangjun. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
Fil: Chakravarthy, Suma. Cornell University; Estados Unidos
Fil: Martin, Gregory B.. Boyce Thompson Institute for Plant Research; Estados Unidos. Cornell University; Estados Unidos
description The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against Pseudomonas syringae pv. tomato (Pst). The tomato genome has two Pti1 genes, referred to as Pti1a and Pti1b. A hairpin-Pti1 (hpPti1) construct was developed and was used to generate two independent stable transgenic tomato lines that had reduced transcript abundance of both genes. In response to P. syringae pv. tomato inoculation, these hpPti1 plants developed more severe disease symptoms, supported higher bacterial populations, and had reduced transcript accumulation of PTI-associated genes, as compared with wild-type plants. In response to two flagellin-derived peptides, the hpPti1 plants produced lesser amounts of reactive oxygen species (ROS) but showed no difference in mitogen-activated protein kinase (MAPK). Synthetic Pti1a and Pti1b genes designed to avoid silencing were transiently expressed in the hpPti1 plants and restored the ability of the plants to produce wild-type levels of ROS. Our results identify a new component of PTI in tomato that, because it affects ROS production but not MAPK signaling, appears to act early in the immune response.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65416
Schwizer, Simon; Kraus, Christine M.; Dunham, Diane M.; Zheng, Yi; Fernandez Pozo, Noé; et al.; The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 30; 9; 9-2017; 725-738
0894-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65416
identifier_str_mv Schwizer, Simon; Kraus, Christine M.; Dunham, Diane M.; Zheng, Yi; Fernandez Pozo, Noé; et al.; The tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 30; 9; 9-2017; 725-738
0894-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-03-17-0056-R
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-17-0056-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268922895663104
score 13.13397

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