Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers

Autores
Nascimento, Juliana; Lima, Jucivaldo D.; Suarez, Pablo; Baldo, Juan Diego; Andrade, Gilda V.; Pierson, Todd W.; Fitzpatrick, Benjamin M.; Baptista Haddad, Célio Fernando; Recco Pimentel, Shirlei M.; Lourenço, Luciana B.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri–P. ephippifer species complex is even greater than previously suspected. Specimens from Viruá and Western Pará, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri–P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.
Fil: Nascimento, Juliana. Universidade Estadual de Campinas. Instituto de Biología; Brasil
Fil: Lima, Jucivaldo D.. Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá; Brasil
Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Andrade, Gilda V.. Universidade Federal Do Maranhao.; Brasil
Fil: Pierson, Todd W.. University of Tennessee; Estados Unidos
Fil: Fitzpatrick, Benjamin M.. University of Tennessee; Estados Unidos
Fil: Baptista Haddad, Célio Fernando. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Recco Pimentel, Shirlei M.. Universidade Estadual de Campinas. Instituto de Biología; Brasil
Fil: Lourenço, Luciana B.. Universidade Estadual de Campinas. Instituto de Biología; Brasil
Materia
DNA-BASED SPECIES DELIMITATION
RADSEQ
AMAZONIA
CHROMOSOME
PHYSALAEMUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109613
Acceder
id CONICETDig_e801ea9cb755044e4594f08c9592289f
oai_identifier_str oai:ri.conicet.gov.ar:11336/109613
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic MarkersNascimento, JulianaLima, Jucivaldo D.Suarez, PabloBaldo, Juan DiegoAndrade, Gilda V.Pierson, Todd W.Fitzpatrick, Benjamin M.Baptista Haddad, Célio FernandoRecco Pimentel, Shirlei M.Lourenço, Luciana B.DNA-BASED SPECIES DELIMITATIONRADSEQAMAZONIACHROMOSOMEPHYSALAEMUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri–P. ephippifer species complex is even greater than previously suspected. Specimens from Viruá and Western Pará, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri–P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.Fil: Nascimento, Juliana. Universidade Estadual de Campinas. Instituto de Biología; BrasilFil: Lima, Jucivaldo D.. Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá; BrasilFil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Andrade, Gilda V.. Universidade Federal Do Maranhao.; BrasilFil: Pierson, Todd W.. University of Tennessee; Estados UnidosFil: Fitzpatrick, Benjamin M.. University of Tennessee; Estados UnidosFil: Baptista Haddad, Célio Fernando. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Recco Pimentel, Shirlei M.. Universidade Estadual de Campinas. Instituto de Biología; BrasilFil: Lourenço, Luciana B.. Universidade Estadual de Campinas. Instituto de Biología; BrasilFrontiers Media S.A.2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109613Nascimento, Juliana; Lima, Jucivaldo D.; Suarez, Pablo; Baldo, Juan Diego; Andrade, Gilda V.; et al.; Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers; Frontiers Media S.A.; Frontiers in Genetics; 10; 719; 8-2019; 1-151664-80211664-8021CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fgene.2019.00719/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fgene.2019.00719info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:45Zoai:ri.conicet.gov.ar:11336/109613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:45.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
title Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
spellingShingle Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
Nascimento, Juliana
DNA-BASED SPECIES DELIMITATION
RADSEQ
AMAZONIA
CHROMOSOME
PHYSALAEMUS
title_short Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
title_full Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
title_fullStr Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
title_full_unstemmed Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
title_sort Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers
dc.creator.none.fl_str_mv Nascimento, Juliana
Lima, Jucivaldo D.
Suarez, Pablo
Baldo, Juan Diego
Andrade, Gilda V.
Pierson, Todd W.
Fitzpatrick, Benjamin M.
Baptista Haddad, Célio Fernando
Recco Pimentel, Shirlei M.
Lourenço, Luciana B.
author Nascimento, Juliana
author_facet Nascimento, Juliana
Lima, Jucivaldo D.
Suarez, Pablo
Baldo, Juan Diego
Andrade, Gilda V.
Pierson, Todd W.
Fitzpatrick, Benjamin M.
Baptista Haddad, Célio Fernando
Recco Pimentel, Shirlei M.
Lourenço, Luciana B.
author_role author
author2 Lima, Jucivaldo D.
Suarez, Pablo
Baldo, Juan Diego
Andrade, Gilda V.
Pierson, Todd W.
Fitzpatrick, Benjamin M.
Baptista Haddad, Célio Fernando
Recco Pimentel, Shirlei M.
Lourenço, Luciana B.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DNA-BASED SPECIES DELIMITATION
RADSEQ
AMAZONIA
CHROMOSOME
PHYSALAEMUS
topic DNA-BASED SPECIES DELIMITATION
RADSEQ
AMAZONIA
CHROMOSOME
PHYSALAEMUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri–P. ephippifer species complex is even greater than previously suspected. Specimens from Viruá and Western Pará, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri–P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.
Fil: Nascimento, Juliana. Universidade Estadual de Campinas. Instituto de Biología; Brasil
Fil: Lima, Jucivaldo D.. Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá; Brasil
Fil: Suarez, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Andrade, Gilda V.. Universidade Federal Do Maranhao.; Brasil
Fil: Pierson, Todd W.. University of Tennessee; Estados Unidos
Fil: Fitzpatrick, Benjamin M.. University of Tennessee; Estados Unidos
Fil: Baptista Haddad, Célio Fernando. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Recco Pimentel, Shirlei M.. Universidade Estadual de Campinas. Instituto de Biología; Brasil
Fil: Lourenço, Luciana B.. Universidade Estadual de Campinas. Instituto de Biología; Brasil
description Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri–P. ephippifer species complex is even greater than previously suspected. Specimens from Viruá and Western Pará, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri–P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109613
Nascimento, Juliana; Lima, Jucivaldo D.; Suarez, Pablo; Baldo, Juan Diego; Andrade, Gilda V.; et al.; Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers; Frontiers Media S.A.; Frontiers in Genetics; 10; 719; 8-2019; 1-15
1664-8021
1664-8021
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109613
identifier_str_mv Nascimento, Juliana; Lima, Jucivaldo D.; Suarez, Pablo; Baldo, Juan Diego; Andrade, Gilda V.; et al.; Extensive Cryptic Diversity Within the Physalaemus cuvieri - Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers; Frontiers Media S.A.; Frontiers in Genetics; 10; 719; 8-2019; 1-15
1664-8021
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fgene.2019.00719/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fgene.2019.00719
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269304910774272
score 13.13397

Publicaciones similares