Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis

Autores
Fraunhoffer Navarro, Nicolas Alejandro; Meilerman Abuelafia, Miriam Analia; Stella, Inés; Galliano, Silvia; Barrios, Marcela; Vitullo, Alfredo Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Endometriosis is a gynaecological disorder that affects 6-10 % of female population. It is characterized by the presence of endometrial tissue outside the uterus, most often in the pelvic peritoneum or ovaries. Recent studies have indicated that mesenchymal endometrial stem cells might get involved in endometriosis progression. Although germ line stem cells have been proved to exist in the ovary, their involvement in ovarian endometriosis has not been investigated. In this preliminary report we aimed to identify germinal stem cell markers in ovarian endometriosis. Findings: Ten paraffin-embedded ovarian endometriosis samples were screened for germ cell-specific proteins DDX4 (VASA) and IFITM3, and its relation with stem cell marker OCT4, proliferation marker PCNA and estrogen receptor alpha (ESR1), by immunohistochemistry, immunofluorescence and PCR. DDX4 and IFITM3 proteins were expressed in isolated cells and clusters of cells in the cortical region of ovarian endometriotic cysts. DDX4 and IFITM3 co-localized in cells from endometriotic stroma, and DDX4/IFITM3-expressing cells were positive for ESR1, OCT4 and PCNA. No cells expressing neither DDX4 nor IFITM3 were detected in normal endometrial tissue. Conclusion: The identification of germ cell-specific proteins DDX4 and IFITM3 provides the first evidence of ovarian-sourced cells in ovarian endometriotic lesions and opens up new directions towards understanding the still confusing pathogenesis of endometriosis.
Fil: Fraunhoffer Navarro, Nicolas Alejandro. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Meilerman Abuelafia, Miriam Analia. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Stella, Inés. Universidad Maimónides; Argentina
Fil: Galliano, Silvia. Universidad Maimónides; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina
Fil: Barrios, Marcela. Universidad Maimónides; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
DDX4
IFITM3
OVARIAN ENDOMETRIOSIS
OVARIAN STEM CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69709
Acceder
id CONICETDig_e7d7edf271e51696c501855c050e5133
oai_identifier_str oai:ri.conicet.gov.ar:11336/69709
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosisFraunhoffer Navarro, Nicolas AlejandroMeilerman Abuelafia, Miriam AnaliaStella, InésGalliano, SilviaBarrios, MarcelaVitullo, Alfredo DanielDDX4IFITM3OVARIAN ENDOMETRIOSISOVARIAN STEM CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Endometriosis is a gynaecological disorder that affects 6-10 % of female population. It is characterized by the presence of endometrial tissue outside the uterus, most often in the pelvic peritoneum or ovaries. Recent studies have indicated that mesenchymal endometrial stem cells might get involved in endometriosis progression. Although germ line stem cells have been proved to exist in the ovary, their involvement in ovarian endometriosis has not been investigated. In this preliminary report we aimed to identify germinal stem cell markers in ovarian endometriosis. Findings: Ten paraffin-embedded ovarian endometriosis samples were screened for germ cell-specific proteins DDX4 (VASA) and IFITM3, and its relation with stem cell marker OCT4, proliferation marker PCNA and estrogen receptor alpha (ESR1), by immunohistochemistry, immunofluorescence and PCR. DDX4 and IFITM3 proteins were expressed in isolated cells and clusters of cells in the cortical region of ovarian endometriotic cysts. DDX4 and IFITM3 co-localized in cells from endometriotic stroma, and DDX4/IFITM3-expressing cells were positive for ESR1, OCT4 and PCNA. No cells expressing neither DDX4 nor IFITM3 were detected in normal endometrial tissue. Conclusion: The identification of germ cell-specific proteins DDX4 and IFITM3 provides the first evidence of ovarian-sourced cells in ovarian endometriotic lesions and opens up new directions towards understanding the still confusing pathogenesis of endometriosis.Fil: Fraunhoffer Navarro, Nicolas Alejandro. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Meilerman Abuelafia, Miriam Analia. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Stella, Inés. Universidad Maimónides; ArgentinaFil: Galliano, Silvia. Universidad Maimónides; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Barrios, Marcela. Universidad Maimónides; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioMed Central2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69709Fraunhoffer Navarro, Nicolas Alejandro; Meilerman Abuelafia, Miriam Analia; Stella, Inés; Galliano, Silvia; Barrios, Marcela; et al.; Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis; BioMed Central; Journal of Ovarian Research; 8; 1; 10-2015; 1-71757-2215CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-015-0193-8info:eu-repo/semantics/altIdentifier/doi/10.1186/s13048-015-0193-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:19:17Zoai:ri.conicet.gov.ar:11336/69709instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:19:17.704CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
title Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
spellingShingle Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
Fraunhoffer Navarro, Nicolas Alejandro
DDX4
IFITM3
OVARIAN ENDOMETRIOSIS
OVARIAN STEM CELLS
title_short Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
title_full Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
title_fullStr Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
title_full_unstemmed Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
title_sort Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
dc.creator.none.fl_str_mv Fraunhoffer Navarro, Nicolas Alejandro
Meilerman Abuelafia, Miriam Analia
Stella, Inés
Galliano, Silvia
Barrios, Marcela
Vitullo, Alfredo Daniel
author Fraunhoffer Navarro, Nicolas Alejandro
author_facet Fraunhoffer Navarro, Nicolas Alejandro
Meilerman Abuelafia, Miriam Analia
Stella, Inés
Galliano, Silvia
Barrios, Marcela
Vitullo, Alfredo Daniel
author_role author
author2 Meilerman Abuelafia, Miriam Analia
Stella, Inés
Galliano, Silvia
Barrios, Marcela
Vitullo, Alfredo Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DDX4
IFITM3
OVARIAN ENDOMETRIOSIS
OVARIAN STEM CELLS
topic DDX4
IFITM3
OVARIAN ENDOMETRIOSIS
OVARIAN STEM CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Endometriosis is a gynaecological disorder that affects 6-10 % of female population. It is characterized by the presence of endometrial tissue outside the uterus, most often in the pelvic peritoneum or ovaries. Recent studies have indicated that mesenchymal endometrial stem cells might get involved in endometriosis progression. Although germ line stem cells have been proved to exist in the ovary, their involvement in ovarian endometriosis has not been investigated. In this preliminary report we aimed to identify germinal stem cell markers in ovarian endometriosis. Findings: Ten paraffin-embedded ovarian endometriosis samples were screened for germ cell-specific proteins DDX4 (VASA) and IFITM3, and its relation with stem cell marker OCT4, proliferation marker PCNA and estrogen receptor alpha (ESR1), by immunohistochemistry, immunofluorescence and PCR. DDX4 and IFITM3 proteins were expressed in isolated cells and clusters of cells in the cortical region of ovarian endometriotic cysts. DDX4 and IFITM3 co-localized in cells from endometriotic stroma, and DDX4/IFITM3-expressing cells were positive for ESR1, OCT4 and PCNA. No cells expressing neither DDX4 nor IFITM3 were detected in normal endometrial tissue. Conclusion: The identification of germ cell-specific proteins DDX4 and IFITM3 provides the first evidence of ovarian-sourced cells in ovarian endometriotic lesions and opens up new directions towards understanding the still confusing pathogenesis of endometriosis.
Fil: Fraunhoffer Navarro, Nicolas Alejandro. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Meilerman Abuelafia, Miriam Analia. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Stella, Inés. Universidad Maimónides; Argentina
Fil: Galliano, Silvia. Universidad Maimónides; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina
Fil: Barrios, Marcela. Universidad Maimónides; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: Endometriosis is a gynaecological disorder that affects 6-10 % of female population. It is characterized by the presence of endometrial tissue outside the uterus, most often in the pelvic peritoneum or ovaries. Recent studies have indicated that mesenchymal endometrial stem cells might get involved in endometriosis progression. Although germ line stem cells have been proved to exist in the ovary, their involvement in ovarian endometriosis has not been investigated. In this preliminary report we aimed to identify germinal stem cell markers in ovarian endometriosis. Findings: Ten paraffin-embedded ovarian endometriosis samples were screened for germ cell-specific proteins DDX4 (VASA) and IFITM3, and its relation with stem cell marker OCT4, proliferation marker PCNA and estrogen receptor alpha (ESR1), by immunohistochemistry, immunofluorescence and PCR. DDX4 and IFITM3 proteins were expressed in isolated cells and clusters of cells in the cortical region of ovarian endometriotic cysts. DDX4 and IFITM3 co-localized in cells from endometriotic stroma, and DDX4/IFITM3-expressing cells were positive for ESR1, OCT4 and PCNA. No cells expressing neither DDX4 nor IFITM3 were detected in normal endometrial tissue. Conclusion: The identification of germ cell-specific proteins DDX4 and IFITM3 provides the first evidence of ovarian-sourced cells in ovarian endometriotic lesions and opens up new directions towards understanding the still confusing pathogenesis of endometriosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69709
Fraunhoffer Navarro, Nicolas Alejandro; Meilerman Abuelafia, Miriam Analia; Stella, Inés; Galliano, Silvia; Barrios, Marcela; et al.; Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis; BioMed Central; Journal of Ovarian Research; 8; 1; 10-2015; 1-7
1757-2215
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69709
identifier_str_mv Fraunhoffer Navarro, Nicolas Alejandro; Meilerman Abuelafia, Miriam Analia; Stella, Inés; Galliano, Silvia; Barrios, Marcela; et al.; Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis; BioMed Central; Journal of Ovarian Research; 8; 1; 10-2015; 1-7
1757-2215
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-015-0193-8
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13048-015-0193-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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