Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)

Autores
Gallo Vaulet, Lucía; Vanstreels, Ralph Eric Thijl; Gallo, Luciana; Entrocassi, Andrea Carolina; Peker, Laura Alejandra; Blanco, Gabriela Silvina; Rago, María Virginia; Rodríguez Fermepin, Marcelo; Uhart, Marcela María
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The family Chlamydiaceae is comprised of obligate intracellular bacteria, some of which are significant pathogens of humans and domestic animals. Magellanic penguins (Spheniscus magellanicus) are susceptible to Chlamydia psittaci outbreaks in captivity, and serological surveys detected antibodies against C. psittaci (or antigenically similar organisms) in wild populations of this species. To investigate the occurrence of Chlamydiaceae in wild Magellanic penguins, 167 cloacal swabs were collected from apparently healthy individuals at four breeding colonies along the coast of Chubut, Argentina. Real-time PCR revealed the presence of DNA from Chlamydiaceae-like bacteria in 61 cloacal swabs (36.5%). Detection did not correlate to nutritional status or to meaningful hematological abnormalities. Isolation in monkey kidney cells was unsuccessful, but DNA sequences for the 16S–23S rRNA region were obtained from one sample. Phylogenetic analysis showed a close relationship to unidentified Chlamydiaceae-like bacteria found in chinstrap penguins (Pygoscelis antarcticus) in Antarctica and seagulls in France and the Bering Sea, and to Chlamydiifrater spp. isolated from flamingos in France. Further studies are necessary to clarify the taxonomy and investigate the epidemiology, pathogenicity, and zoonotic potential of this group of Chlamydiaceae-like bacteria.
Fil: Gallo Vaulet, Lucía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Vanstreels, Ralph Eric Thijl. University of California at Davis; Estados Unidos
Fil: Gallo, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Entrocassi, Andrea Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Peker, Laura Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Blanco, Gabriela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Rago, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Rodríguez Fermepin, Marcelo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Uhart, Marcela María. University of California at Davis; Estados Unidos
Materia
CHLAMYDIOSIS
MICROBIOTA
REAL-TIME PCR
SEABIRD
SOUTH AMERICA
WILD BIRD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/205861
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/205861
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)Gallo Vaulet, LucíaVanstreels, Ralph Eric ThijlGallo, LucianaEntrocassi, Andrea CarolinaPeker, Laura AlejandraBlanco, Gabriela SilvinaRago, María VirginiaRodríguez Fermepin, MarceloUhart, Marcela MaríaCHLAMYDIOSISMICROBIOTAREAL-TIME PCRSEABIRDSOUTH AMERICAWILD BIRDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The family Chlamydiaceae is comprised of obligate intracellular bacteria, some of which are significant pathogens of humans and domestic animals. Magellanic penguins (Spheniscus magellanicus) are susceptible to Chlamydia psittaci outbreaks in captivity, and serological surveys detected antibodies against C. psittaci (or antigenically similar organisms) in wild populations of this species. To investigate the occurrence of Chlamydiaceae in wild Magellanic penguins, 167 cloacal swabs were collected from apparently healthy individuals at four breeding colonies along the coast of Chubut, Argentina. Real-time PCR revealed the presence of DNA from Chlamydiaceae-like bacteria in 61 cloacal swabs (36.5%). Detection did not correlate to nutritional status or to meaningful hematological abnormalities. Isolation in monkey kidney cells was unsuccessful, but DNA sequences for the 16S–23S rRNA region were obtained from one sample. Phylogenetic analysis showed a close relationship to unidentified Chlamydiaceae-like bacteria found in chinstrap penguins (Pygoscelis antarcticus) in Antarctica and seagulls in France and the Bering Sea, and to Chlamydiifrater spp. isolated from flamingos in France. Further studies are necessary to clarify the taxonomy and investigate the epidemiology, pathogenicity, and zoonotic potential of this group of Chlamydiaceae-like bacteria.Fil: Gallo Vaulet, Lucía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Vanstreels, Ralph Eric Thijl. University of California at Davis; Estados UnidosFil: Gallo, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: Entrocassi, Andrea Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Peker, Laura Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Blanco, Gabriela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Rago, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Rodríguez Fermepin, Marcelo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Uhart, Marcela María. University of California at Davis; Estados UnidosMDPI2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/205861Gallo Vaulet, Lucía; Vanstreels, Ralph Eric Thijl; Gallo, Luciana; Entrocassi, Andrea Carolina; Peker, Laura Alejandra; et al.; Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus); MDPI; Diversity; 14; 9; 9-2022; 1-91424-2818CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/d14090746info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-2818/14/9/746info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:11Zoai:ri.conicet.gov.ar:11336/205861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:12.246CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
title Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
spellingShingle Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
Gallo Vaulet, Lucía
CHLAMYDIOSIS
MICROBIOTA
REAL-TIME PCR
SEABIRD
SOUTH AMERICA
WILD BIRD
title_short Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
title_full Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
title_fullStr Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
title_full_unstemmed Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
title_sort Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus)
dc.creator.none.fl_str_mv Gallo Vaulet, Lucía
Vanstreels, Ralph Eric Thijl
Gallo, Luciana
Entrocassi, Andrea Carolina
Peker, Laura Alejandra
Blanco, Gabriela Silvina
Rago, María Virginia
Rodríguez Fermepin, Marcelo
Uhart, Marcela María
author Gallo Vaulet, Lucía
author_facet Gallo Vaulet, Lucía
Vanstreels, Ralph Eric Thijl
Gallo, Luciana
Entrocassi, Andrea Carolina
Peker, Laura Alejandra
Blanco, Gabriela Silvina
Rago, María Virginia
Rodríguez Fermepin, Marcelo
Uhart, Marcela María
author_role author
author2 Vanstreels, Ralph Eric Thijl
Gallo, Luciana
Entrocassi, Andrea Carolina
Peker, Laura Alejandra
Blanco, Gabriela Silvina
Rago, María Virginia
Rodríguez Fermepin, Marcelo
Uhart, Marcela María
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHLAMYDIOSIS
MICROBIOTA
REAL-TIME PCR
SEABIRD
SOUTH AMERICA
WILD BIRD
topic CHLAMYDIOSIS
MICROBIOTA
REAL-TIME PCR
SEABIRD
SOUTH AMERICA
WILD BIRD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The family Chlamydiaceae is comprised of obligate intracellular bacteria, some of which are significant pathogens of humans and domestic animals. Magellanic penguins (Spheniscus magellanicus) are susceptible to Chlamydia psittaci outbreaks in captivity, and serological surveys detected antibodies against C. psittaci (or antigenically similar organisms) in wild populations of this species. To investigate the occurrence of Chlamydiaceae in wild Magellanic penguins, 167 cloacal swabs were collected from apparently healthy individuals at four breeding colonies along the coast of Chubut, Argentina. Real-time PCR revealed the presence of DNA from Chlamydiaceae-like bacteria in 61 cloacal swabs (36.5%). Detection did not correlate to nutritional status or to meaningful hematological abnormalities. Isolation in monkey kidney cells was unsuccessful, but DNA sequences for the 16S–23S rRNA region were obtained from one sample. Phylogenetic analysis showed a close relationship to unidentified Chlamydiaceae-like bacteria found in chinstrap penguins (Pygoscelis antarcticus) in Antarctica and seagulls in France and the Bering Sea, and to Chlamydiifrater spp. isolated from flamingos in France. Further studies are necessary to clarify the taxonomy and investigate the epidemiology, pathogenicity, and zoonotic potential of this group of Chlamydiaceae-like bacteria.
Fil: Gallo Vaulet, Lucía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Vanstreels, Ralph Eric Thijl. University of California at Davis; Estados Unidos
Fil: Gallo, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Entrocassi, Andrea Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Peker, Laura Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Blanco, Gabriela Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Rago, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Rodríguez Fermepin, Marcelo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Uhart, Marcela María. University of California at Davis; Estados Unidos
description The family Chlamydiaceae is comprised of obligate intracellular bacteria, some of which are significant pathogens of humans and domestic animals. Magellanic penguins (Spheniscus magellanicus) are susceptible to Chlamydia psittaci outbreaks in captivity, and serological surveys detected antibodies against C. psittaci (or antigenically similar organisms) in wild populations of this species. To investigate the occurrence of Chlamydiaceae in wild Magellanic penguins, 167 cloacal swabs were collected from apparently healthy individuals at four breeding colonies along the coast of Chubut, Argentina. Real-time PCR revealed the presence of DNA from Chlamydiaceae-like bacteria in 61 cloacal swabs (36.5%). Detection did not correlate to nutritional status or to meaningful hematological abnormalities. Isolation in monkey kidney cells was unsuccessful, but DNA sequences for the 16S–23S rRNA region were obtained from one sample. Phylogenetic analysis showed a close relationship to unidentified Chlamydiaceae-like bacteria found in chinstrap penguins (Pygoscelis antarcticus) in Antarctica and seagulls in France and the Bering Sea, and to Chlamydiifrater spp. isolated from flamingos in France. Further studies are necessary to clarify the taxonomy and investigate the epidemiology, pathogenicity, and zoonotic potential of this group of Chlamydiaceae-like bacteria.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/205861
Gallo Vaulet, Lucía; Vanstreels, Ralph Eric Thijl; Gallo, Luciana; Entrocassi, Andrea Carolina; Peker, Laura Alejandra; et al.; Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus); MDPI; Diversity; 14; 9; 9-2022; 1-9
1424-2818
CONICET Digital
CONICET
url http://hdl.handle.net/11336/205861
identifier_str_mv Gallo Vaulet, Lucía; Vanstreels, Ralph Eric Thijl; Gallo, Luciana; Entrocassi, Andrea Carolina; Peker, Laura Alejandra; et al.; Chlamydiaceae-Like Bacterium in Wild Magellanic Penguins (Spheniscus magellanicus); MDPI; Diversity; 14; 9; 9-2022; 1-9
1424-2818
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/d14090746
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-2818/14/9/746
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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