Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2

Autores
Curti, Lucía Ana; Primost, Ivana; Valla, Sofía Aylén; Ibañez Alegre, Daiana Macarena; Olguin Perglione, Cecilia; Repizo, Guillermo Daniel; Lara, María Julia; Parcerisa, Ivana Lorna; Palacios, Antonela Rocio; Llases, María Eugenia; Rinflerch, Adriana Raquel; Barrios, Melanie; Pereyra Bonnet, Federico Alberto; Giménez, Carla Alejandra; Marcone, Débora Natalia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the LyoCRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.
Fil: Curti, Lucía Ana. Caspr Biotech; Estados Unidos
Fil: Primost, Ivana. Municipalidad de Malvinas Argentinas (buenos Aires). Hospital Municipal de Trauma y Emergencias Doctor Federico Abete.; Argentina
Fil: Valla, Sofía Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Ibañez Alegre, Daiana Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Repizo, Guillermo Daniel. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lara, María Julia. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parcerisa, Ivana Lorna. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacios, Antonela Rocio. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Llases, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Caspr Biotech; Estados Unidos
Fil: Rinflerch, Adriana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Barrios, Melanie. Instituto de Producción Agropecuaria; Argentina
Fil: Pereyra Bonnet, Federico Alberto. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giménez, Carla Alejandra. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marcone, Débora Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
COVID-19
CRISPR
DIAGNOSIS
FLUORESCENCE DETECTION
LYSIS BUFFER
SARS-COV-2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/135603

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network_name_str CONICET Digital (CONICET)
spelling Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2Curti, Lucía AnaPrimost, IvanaValla, Sofía AylénIbañez Alegre, Daiana MacarenaOlguin Perglione, CeciliaRepizo, Guillermo DanielLara, María JuliaParcerisa, Ivana LornaPalacios, Antonela RocioLlases, María EugeniaRinflerch, Adriana RaquelBarrios, MelaniePereyra Bonnet, Federico AlbertoGiménez, Carla AlejandraMarcone, Débora NataliaCOVID-19CRISPRDIAGNOSISFLUORESCENCE DETECTIONLYSIS BUFFERSARS-COV-2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the LyoCRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.Fil: Curti, Lucía Ana. Caspr Biotech; Estados UnidosFil: Primost, Ivana. Municipalidad de Malvinas Argentinas (buenos Aires). Hospital Municipal de Trauma y Emergencias Doctor Federico Abete.; ArgentinaFil: Valla, Sofía Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Ibañez Alegre, Daiana Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Repizo, Guillermo Daniel. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lara, María Julia. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Parcerisa, Ivana Lorna. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palacios, Antonela Rocio. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Llases, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Caspr Biotech; Estados UnidosFil: Rinflerch, Adriana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Barrios, Melanie. Instituto de Producción Agropecuaria; ArgentinaFil: Pereyra Bonnet, Federico Alberto. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giménez, Carla Alejandra. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marcone, Débora Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMultidisciplinary Digital Publishing Institute2021-03-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/135603Curti, Lucía Ana; Primost, Ivana; Valla, Sofía Aylén; Ibañez Alegre, Daiana Macarena; Olguin Perglione, Cecilia; et al.; Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2; Multidisciplinary Digital Publishing Institute; Viruses; 13; 3; 5-3-2021; 1-91999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/13/3/420info:eu-repo/semantics/altIdentifier/doi/10.3390/v13030420info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:06Zoai:ri.conicet.gov.ar:11336/135603instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:06.871CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
title Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
spellingShingle Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
Curti, Lucía Ana
COVID-19
CRISPR
DIAGNOSIS
FLUORESCENCE DETECTION
LYSIS BUFFER
SARS-COV-2
title_short Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
title_full Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
title_fullStr Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
title_full_unstemmed Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
title_sort Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
dc.creator.none.fl_str_mv Curti, Lucía Ana
Primost, Ivana
Valla, Sofía Aylén
Ibañez Alegre, Daiana Macarena
Olguin Perglione, Cecilia
Repizo, Guillermo Daniel
Lara, María Julia
Parcerisa, Ivana Lorna
Palacios, Antonela Rocio
Llases, María Eugenia
Rinflerch, Adriana Raquel
Barrios, Melanie
Pereyra Bonnet, Federico Alberto
Giménez, Carla Alejandra
Marcone, Débora Natalia
author Curti, Lucía Ana
author_facet Curti, Lucía Ana
Primost, Ivana
Valla, Sofía Aylén
Ibañez Alegre, Daiana Macarena
Olguin Perglione, Cecilia
Repizo, Guillermo Daniel
Lara, María Julia
Parcerisa, Ivana Lorna
Palacios, Antonela Rocio
Llases, María Eugenia
Rinflerch, Adriana Raquel
Barrios, Melanie
Pereyra Bonnet, Federico Alberto
Giménez, Carla Alejandra
Marcone, Débora Natalia
author_role author
author2 Primost, Ivana
Valla, Sofía Aylén
Ibañez Alegre, Daiana Macarena
Olguin Perglione, Cecilia
Repizo, Guillermo Daniel
Lara, María Julia
Parcerisa, Ivana Lorna
Palacios, Antonela Rocio
Llases, María Eugenia
Rinflerch, Adriana Raquel
Barrios, Melanie
Pereyra Bonnet, Federico Alberto
Giménez, Carla Alejandra
Marcone, Débora Natalia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COVID-19
CRISPR
DIAGNOSIS
FLUORESCENCE DETECTION
LYSIS BUFFER
SARS-COV-2
topic COVID-19
CRISPR
DIAGNOSIS
FLUORESCENCE DETECTION
LYSIS BUFFER
SARS-COV-2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the LyoCRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.
Fil: Curti, Lucía Ana. Caspr Biotech; Estados Unidos
Fil: Primost, Ivana. Municipalidad de Malvinas Argentinas (buenos Aires). Hospital Municipal de Trauma y Emergencias Doctor Federico Abete.; Argentina
Fil: Valla, Sofía Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Ibañez Alegre, Daiana Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Repizo, Guillermo Daniel. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lara, María Julia. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parcerisa, Ivana Lorna. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacios, Antonela Rocio. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Llases, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Caspr Biotech; Estados Unidos
Fil: Rinflerch, Adriana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Barrios, Melanie. Instituto de Producción Agropecuaria; Argentina
Fil: Pereyra Bonnet, Federico Alberto. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giménez, Carla Alejandra. Caspr Biotech; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marcone, Débora Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the LyoCRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/135603
Curti, Lucía Ana; Primost, Ivana; Valla, Sofía Aylén; Ibañez Alegre, Daiana Macarena; Olguin Perglione, Cecilia; et al.; Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2; Multidisciplinary Digital Publishing Institute; Viruses; 13; 3; 5-3-2021; 1-9
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/135603
identifier_str_mv Curti, Lucía Ana; Primost, Ivana; Valla, Sofía Aylén; Ibañez Alegre, Daiana Macarena; Olguin Perglione, Cecilia; et al.; Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2; Multidisciplinary Digital Publishing Institute; Viruses; 13; 3; 5-3-2021; 1-9
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/13/3/420
info:eu-repo/semantics/altIdentifier/doi/10.3390/v13030420
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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