Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
- Autores
- Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; Gohmert, Melanie; Swallow, Maxine; Tufa, Dejene; Jacobs, Roland; Lienenklaus, Stefan; Kühl, Anja A.; Borkner, Lisa; Cicin-Sain, Luka; Holzmann, Bernard; Wagner, Hermann; Berod, Luciana; Sparwasser, Tim
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity.
Fil: Puttur, Franz. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Francozo, Marcela. Centre for Experimental and Clinical Infection Research; Alemania. Universidade de Sao Paulo; Brasil
Fil: Solmaz, Gülhas. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Lindenberg, Marc. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Gohmert, Melanie. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Swallow, Maxine. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Tufa, Dejene. Department of Clinical Immunology and Rheumatology; Alemania
Fil: Jacobs, Roland. Department of Clinical Immunology and Rheumatology; Alemania
Fil: Lienenklaus, Stefan. Institute for Laboratory Animal Science; Alemania. Institute for Experimental Infection Research; Alemania
Fil: Kühl, Anja A.. Charité–Universitätsmedizin Berlin; Alemania
Fil: Borkner, Lisa. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania
Fil: Cicin-Sain, Luka. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania
Fil: Holzmann, Bernard. Technische Universitat Munchen; Alemania
Fil: Wagner, Hermann. Technische Universitat Munchen; Alemania
Fil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Sparwasser, Tim. Centre for Experimental and Clinical Infection Research; Alemania - Materia
-
Conventional Dc
Mcmv
Nk Cells
Plasmacytoid Dc
Tlr9-Myd88 Signaling - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/62282
Ver los metadatos del registro completo
id |
CONICETDig_e5d015fab1ff14645e6874126a56fc4a |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/62282 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 SignalingPuttur, FranzFrancozo, MarcelaSolmaz, GülhasBueno, Carlos AlbertoLindenberg, MarcGohmert, MelanieSwallow, MaxineTufa, DejeneJacobs, RolandLienenklaus, StefanKühl, Anja A.Borkner, LisaCicin-Sain, LukaHolzmann, BernardWagner, HermannBerod, LucianaSparwasser, TimConventional DcMcmvNk CellsPlasmacytoid DcTlr9-Myd88 Signalinghttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity.Fil: Puttur, Franz. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Francozo, Marcela. Centre for Experimental and Clinical Infection Research; Alemania. Universidade de Sao Paulo; BrasilFil: Solmaz, Gülhas. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Lindenberg, Marc. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Gohmert, Melanie. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Swallow, Maxine. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Tufa, Dejene. Department of Clinical Immunology and Rheumatology; AlemaniaFil: Jacobs, Roland. Department of Clinical Immunology and Rheumatology; AlemaniaFil: Lienenklaus, Stefan. Institute for Laboratory Animal Science; Alemania. Institute for Experimental Infection Research; AlemaniaFil: Kühl, Anja A.. Charité–Universitätsmedizin Berlin; AlemaniaFil: Borkner, Lisa. Department for Vaccinology/Immune Aging and Chronic Infection; AlemaniaFil: Cicin-Sain, Luka. Department for Vaccinology/Immune Aging and Chronic Infection; AlemaniaFil: Holzmann, Bernard. Technische Universitat Munchen; AlemaniaFil: Wagner, Hermann. Technische Universitat Munchen; AlemaniaFil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Sparwasser, Tim. Centre for Experimental and Clinical Infection Research; AlemaniaElsevier Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62282Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; et al.; Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling; Elsevier Science; Cell Reports; 17; 4; 10-2016; 1113-11272211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.09.055info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124716312943info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:09Zoai:ri.conicet.gov.ar:11336/62282instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:10.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
title |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
spellingShingle |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling Puttur, Franz Conventional Dc Mcmv Nk Cells Plasmacytoid Dc Tlr9-Myd88 Signaling |
title_short |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
title_full |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
title_fullStr |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
title_full_unstemmed |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
title_sort |
Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling |
dc.creator.none.fl_str_mv |
Puttur, Franz Francozo, Marcela Solmaz, Gülhas Bueno, Carlos Alberto Lindenberg, Marc Gohmert, Melanie Swallow, Maxine Tufa, Dejene Jacobs, Roland Lienenklaus, Stefan Kühl, Anja A. Borkner, Lisa Cicin-Sain, Luka Holzmann, Bernard Wagner, Hermann Berod, Luciana Sparwasser, Tim |
author |
Puttur, Franz |
author_facet |
Puttur, Franz Francozo, Marcela Solmaz, Gülhas Bueno, Carlos Alberto Lindenberg, Marc Gohmert, Melanie Swallow, Maxine Tufa, Dejene Jacobs, Roland Lienenklaus, Stefan Kühl, Anja A. Borkner, Lisa Cicin-Sain, Luka Holzmann, Bernard Wagner, Hermann Berod, Luciana Sparwasser, Tim |
author_role |
author |
author2 |
Francozo, Marcela Solmaz, Gülhas Bueno, Carlos Alberto Lindenberg, Marc Gohmert, Melanie Swallow, Maxine Tufa, Dejene Jacobs, Roland Lienenklaus, Stefan Kühl, Anja A. Borkner, Lisa Cicin-Sain, Luka Holzmann, Bernard Wagner, Hermann Berod, Luciana Sparwasser, Tim |
author2_role |
author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Conventional Dc Mcmv Nk Cells Plasmacytoid Dc Tlr9-Myd88 Signaling |
topic |
Conventional Dc Mcmv Nk Cells Plasmacytoid Dc Tlr9-Myd88 Signaling |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity. Fil: Puttur, Franz. Centre for Experimental and Clinical Infection Research; Alemania Fil: Francozo, Marcela. Centre for Experimental and Clinical Infection Research; Alemania. Universidade de Sao Paulo; Brasil Fil: Solmaz, Gülhas. Centre for Experimental and Clinical Infection Research; Alemania Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Centre for Experimental and Clinical Infection Research; Alemania Fil: Lindenberg, Marc. Centre for Experimental and Clinical Infection Research; Alemania Fil: Gohmert, Melanie. Centre for Experimental and Clinical Infection Research; Alemania Fil: Swallow, Maxine. Centre for Experimental and Clinical Infection Research; Alemania Fil: Tufa, Dejene. Department of Clinical Immunology and Rheumatology; Alemania Fil: Jacobs, Roland. Department of Clinical Immunology and Rheumatology; Alemania Fil: Lienenklaus, Stefan. Institute for Laboratory Animal Science; Alemania. Institute for Experimental Infection Research; Alemania Fil: Kühl, Anja A.. Charité–Universitätsmedizin Berlin; Alemania Fil: Borkner, Lisa. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania Fil: Cicin-Sain, Luka. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania Fil: Holzmann, Bernard. Technische Universitat Munchen; Alemania Fil: Wagner, Hermann. Technische Universitat Munchen; Alemania Fil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania Fil: Sparwasser, Tim. Centre for Experimental and Clinical Infection Research; Alemania |
description |
Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/62282 Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; et al.; Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling; Elsevier Science; Cell Reports; 17; 4; 10-2016; 1113-1127 2211-1247 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/62282 |
identifier_str_mv |
Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; et al.; Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling; Elsevier Science; Cell Reports; 17; 4; 10-2016; 1113-1127 2211-1247 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.09.055 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124716312943 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613470085971968 |
score |
13.070432 |