Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling

Autores
Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; Gohmert, Melanie; Swallow, Maxine; Tufa, Dejene; Jacobs, Roland; Lienenklaus, Stefan; Kühl, Anja A.; Borkner, Lisa; Cicin-Sain, Luka; Holzmann, Bernard; Wagner, Hermann; Berod, Luciana; Sparwasser, Tim
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity.
Fil: Puttur, Franz. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Francozo, Marcela. Centre for Experimental and Clinical Infection Research; Alemania. Universidade de Sao Paulo; Brasil
Fil: Solmaz, Gülhas. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Lindenberg, Marc. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Gohmert, Melanie. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Swallow, Maxine. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Tufa, Dejene. Department of Clinical Immunology and Rheumatology; Alemania
Fil: Jacobs, Roland. Department of Clinical Immunology and Rheumatology; Alemania
Fil: Lienenklaus, Stefan. Institute for Laboratory Animal Science; Alemania. Institute for Experimental Infection Research; Alemania
Fil: Kühl, Anja A.. Charité–Universitätsmedizin Berlin; Alemania
Fil: Borkner, Lisa. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania
Fil: Cicin-Sain, Luka. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania
Fil: Holzmann, Bernard. Technische Universitat Munchen; Alemania
Fil: Wagner, Hermann. Technische Universitat Munchen; Alemania
Fil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Sparwasser, Tim. Centre for Experimental and Clinical Infection Research; Alemania
Materia
Conventional Dc
Mcmv
Nk Cells
Plasmacytoid Dc
Tlr9-Myd88 Signaling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62282
Acceder
id CONICETDig_e5d015fab1ff14645e6874126a56fc4a
oai_identifier_str oai:ri.conicet.gov.ar:11336/62282
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 SignalingPuttur, FranzFrancozo, MarcelaSolmaz, GülhasBueno, Carlos AlbertoLindenberg, MarcGohmert, MelanieSwallow, MaxineTufa, DejeneJacobs, RolandLienenklaus, StefanKühl, Anja A.Borkner, LisaCicin-Sain, LukaHolzmann, BernardWagner, HermannBerod, LucianaSparwasser, TimConventional DcMcmvNk CellsPlasmacytoid DcTlr9-Myd88 Signalinghttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity.Fil: Puttur, Franz. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Francozo, Marcela. Centre for Experimental and Clinical Infection Research; Alemania. Universidade de Sao Paulo; BrasilFil: Solmaz, Gülhas. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Lindenberg, Marc. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Gohmert, Melanie. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Swallow, Maxine. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Tufa, Dejene. Department of Clinical Immunology and Rheumatology; AlemaniaFil: Jacobs, Roland. Department of Clinical Immunology and Rheumatology; AlemaniaFil: Lienenklaus, Stefan. Institute for Laboratory Animal Science; Alemania. Institute for Experimental Infection Research; AlemaniaFil: Kühl, Anja A.. Charité–Universitätsmedizin Berlin; AlemaniaFil: Borkner, Lisa. Department for Vaccinology/Immune Aging and Chronic Infection; AlemaniaFil: Cicin-Sain, Luka. Department for Vaccinology/Immune Aging and Chronic Infection; AlemaniaFil: Holzmann, Bernard. Technische Universitat Munchen; AlemaniaFil: Wagner, Hermann. Technische Universitat Munchen; AlemaniaFil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; AlemaniaFil: Sparwasser, Tim. Centre for Experimental and Clinical Infection Research; AlemaniaElsevier Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62282Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; et al.; Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling; Elsevier Science; Cell Reports; 17; 4; 10-2016; 1113-11272211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.09.055info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124716312943info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:16:01Zoai:ri.conicet.gov.ar:11336/62282instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:16:02.251CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
title Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
spellingShingle Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
Puttur, Franz
Conventional Dc
Mcmv
Nk Cells
Plasmacytoid Dc
Tlr9-Myd88 Signaling
title_short Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
title_full Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
title_fullStr Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
title_full_unstemmed Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
title_sort Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling
dc.creator.none.fl_str_mv Puttur, Franz
Francozo, Marcela
Solmaz, Gülhas
Bueno, Carlos Alberto
Lindenberg, Marc
Gohmert, Melanie
Swallow, Maxine
Tufa, Dejene
Jacobs, Roland
Lienenklaus, Stefan
Kühl, Anja A.
Borkner, Lisa
Cicin-Sain, Luka
Holzmann, Bernard
Wagner, Hermann
Berod, Luciana
Sparwasser, Tim
author Puttur, Franz
author_facet Puttur, Franz
Francozo, Marcela
Solmaz, Gülhas
Bueno, Carlos Alberto
Lindenberg, Marc
Gohmert, Melanie
Swallow, Maxine
Tufa, Dejene
Jacobs, Roland
Lienenklaus, Stefan
Kühl, Anja A.
Borkner, Lisa
Cicin-Sain, Luka
Holzmann, Bernard
Wagner, Hermann
Berod, Luciana
Sparwasser, Tim
author_role author
author2 Francozo, Marcela
Solmaz, Gülhas
Bueno, Carlos Alberto
Lindenberg, Marc
Gohmert, Melanie
Swallow, Maxine
Tufa, Dejene
Jacobs, Roland
Lienenklaus, Stefan
Kühl, Anja A.
Borkner, Lisa
Cicin-Sain, Luka
Holzmann, Bernard
Wagner, Hermann
Berod, Luciana
Sparwasser, Tim
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Conventional Dc
Mcmv
Nk Cells
Plasmacytoid Dc
Tlr9-Myd88 Signaling
topic Conventional Dc
Mcmv
Nk Cells
Plasmacytoid Dc
Tlr9-Myd88 Signaling
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity.
Fil: Puttur, Franz. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Francozo, Marcela. Centre for Experimental and Clinical Infection Research; Alemania. Universidade de Sao Paulo; Brasil
Fil: Solmaz, Gülhas. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Bueno, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Lindenberg, Marc. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Gohmert, Melanie. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Swallow, Maxine. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Tufa, Dejene. Department of Clinical Immunology and Rheumatology; Alemania
Fil: Jacobs, Roland. Department of Clinical Immunology and Rheumatology; Alemania
Fil: Lienenklaus, Stefan. Institute for Laboratory Animal Science; Alemania. Institute for Experimental Infection Research; Alemania
Fil: Kühl, Anja A.. Charité–Universitätsmedizin Berlin; Alemania
Fil: Borkner, Lisa. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania
Fil: Cicin-Sain, Luka. Department for Vaccinology/Immune Aging and Chronic Infection; Alemania
Fil: Holzmann, Bernard. Technische Universitat Munchen; Alemania
Fil: Wagner, Hermann. Technische Universitat Munchen; Alemania
Fil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania
Fil: Sparwasser, Tim. Centre for Experimental and Clinical Infection Research; Alemania
description Cytomegalovirus (CMV) is an opportunistic virus severely infecting immunocompromised individuals. In mice, endosomal Toll-like receptor 9 (TLR9) and downstream myeloid differentiation factor 88 (MyD88) are central to activating innate immune responses against mouse CMV (MCMV). In this respect, the cell-specific contribution of these pathways in initiating anti-MCMV immunity remains unclear. Using transgenic mice, we demonstrate that TLR9/MyD88 signaling selectively in CD11c+ dendritic cells (DCs) strongly enhances MCMV clearance by boosting natural killer (NK) cell CD69 expression and IFN-γ production. In addition, we show that in the absence of plasmacytoid DCs (pDCs), conventional DCs (cDCs) promote robust NK cell effector function and MCMV clearance in a TLR9/MyD88-dependent manner. Simultaneously, cDC-derived IL-15 regulates NK cell degranulation by TLR9/MyD88-independent mechanisms. Overall, we compartmentalize the cellular contribution of TLR9 and MyD88 signaling in individual DC subsets and evaluate the mechanism by which cDCs control MCMV immunity.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62282
Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; et al.; Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling; Elsevier Science; Cell Reports; 17; 4; 10-2016; 1113-1127
2211-1247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62282
identifier_str_mv Puttur, Franz; Francozo, Marcela; Solmaz, Gülhas; Bueno, Carlos Alberto; Lindenberg, Marc; et al.; Conventional Dendritic Cells Confer Protection against Mouse Cytomegalovirus Infection via TLR9 and MyD88 Signaling; Elsevier Science; Cell Reports; 17; 4; 10-2016; 1113-1127
2211-1247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.09.055
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124716312943
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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