Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice

Autores
Luchetti, Carolina Griselda; Paz, Dante Agustin; Motta, Alicia Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to study the effects of dehydroepiandrosterone (DHEA) and metformin (M) on nitric oxide (NO) system and oxidative stress in embryo implantation sites of early pregnant mice. The biguanide M is used for treating polycystic ovary syndrome but its complete mechanism of action remains unknown. Nitric oxide (NO) has important protective roles during pregnancy, keeping uterine relaxation and vascular function. However, its overproduction leads to nitrative stress by producing reactive nitrogen species. Here we measured NO content by Griess method and the localization of inducible and endothelial nitric oxide synthase (iNOS and eNOS) by immunohistochemistry in implantation sites. Also we measured lipid peroxidation by TBA-RS, glutathion by Ellman’s reaction and antioxidant enzymes by enzymatic kinetics in uterine homogenates. We found that the expression of both iNOS and eNOS and the NO content were increased with DHEA (p<0.001 for all) and restored to control levels with DHEA+M. Oxidative stress: DHEA increased lipid peroxidation (p<0.01) and glutathione (GSH, p<0.01). With DHEA+M lipid peroxidation was restored to control levels. The activities of the antioxidant enzymes superoxide dismutase and catalase were not modified. We conclude that hyperandrogenization with DHEA enhances the NO system and lipid peroxidation in implantation sites of early pregnant mice and that M treatment prevents these effects.
Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Materia
Pregnancy
Metformin
Nitric oxide
Oxidative stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20172
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20172
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant miceLuchetti, Carolina GriseldaPaz, Dante AgustinMotta, Alicia BeatrizPregnancyMetforminNitric oxideOxidative stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to study the effects of dehydroepiandrosterone (DHEA) and metformin (M) on nitric oxide (NO) system and oxidative stress in embryo implantation sites of early pregnant mice. The biguanide M is used for treating polycystic ovary syndrome but its complete mechanism of action remains unknown. Nitric oxide (NO) has important protective roles during pregnancy, keeping uterine relaxation and vascular function. However, its overproduction leads to nitrative stress by producing reactive nitrogen species. Here we measured NO content by Griess method and the localization of inducible and endothelial nitric oxide synthase (iNOS and eNOS) by immunohistochemistry in implantation sites. Also we measured lipid peroxidation by TBA-RS, glutathion by Ellman’s reaction and antioxidant enzymes by enzymatic kinetics in uterine homogenates. We found that the expression of both iNOS and eNOS and the NO content were increased with DHEA (p<0.001 for all) and restored to control levels with DHEA+M. Oxidative stress: DHEA increased lipid peroxidation (p<0.01) and glutathione (GSH, p<0.01). With DHEA+M lipid peroxidation was restored to control levels. The activities of the antioxidant enzymes superoxide dismutase and catalase were not modified. We conclude that hyperandrogenization with DHEA enhances the NO system and lipid peroxidation in implantation sites of early pregnant mice and that M treatment prevents these effects.Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaBentham Science Publishers2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20172Luchetti, Carolina Griselda; Paz, Dante Agustin; Motta, Alicia Beatriz; Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice; Bentham Science Publishers; The Open Reproductive Science Journal; 7; 9-2013; 14-221874-2556CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2174/1874255620130913002info:eu-repo/semantics/altIdentifier/url/https://benthamopen.com/ABSTRACT/TORSJ-5-14info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:08Zoai:ri.conicet.gov.ar:11336/20172instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:08.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
title Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
spellingShingle Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
Luchetti, Carolina Griselda
Pregnancy
Metformin
Nitric oxide
Oxidative stress
title_short Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
title_full Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
title_fullStr Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
title_full_unstemmed Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
title_sort Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice
dc.creator.none.fl_str_mv Luchetti, Carolina Griselda
Paz, Dante Agustin
Motta, Alicia Beatriz
author Luchetti, Carolina Griselda
author_facet Luchetti, Carolina Griselda
Paz, Dante Agustin
Motta, Alicia Beatriz
author_role author
author2 Paz, Dante Agustin
Motta, Alicia Beatriz
author2_role author
author
dc.subject.none.fl_str_mv Pregnancy
Metformin
Nitric oxide
Oxidative stress
topic Pregnancy
Metformin
Nitric oxide
Oxidative stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was to study the effects of dehydroepiandrosterone (DHEA) and metformin (M) on nitric oxide (NO) system and oxidative stress in embryo implantation sites of early pregnant mice. The biguanide M is used for treating polycystic ovary syndrome but its complete mechanism of action remains unknown. Nitric oxide (NO) has important protective roles during pregnancy, keeping uterine relaxation and vascular function. However, its overproduction leads to nitrative stress by producing reactive nitrogen species. Here we measured NO content by Griess method and the localization of inducible and endothelial nitric oxide synthase (iNOS and eNOS) by immunohistochemistry in implantation sites. Also we measured lipid peroxidation by TBA-RS, glutathion by Ellman’s reaction and antioxidant enzymes by enzymatic kinetics in uterine homogenates. We found that the expression of both iNOS and eNOS and the NO content were increased with DHEA (p<0.001 for all) and restored to control levels with DHEA+M. Oxidative stress: DHEA increased lipid peroxidation (p<0.01) and glutathione (GSH, p<0.01). With DHEA+M lipid peroxidation was restored to control levels. The activities of the antioxidant enzymes superoxide dismutase and catalase were not modified. We conclude that hyperandrogenization with DHEA enhances the NO system and lipid peroxidation in implantation sites of early pregnant mice and that M treatment prevents these effects.
Fil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
description The aim of this work was to study the effects of dehydroepiandrosterone (DHEA) and metformin (M) on nitric oxide (NO) system and oxidative stress in embryo implantation sites of early pregnant mice. The biguanide M is used for treating polycystic ovary syndrome but its complete mechanism of action remains unknown. Nitric oxide (NO) has important protective roles during pregnancy, keeping uterine relaxation and vascular function. However, its overproduction leads to nitrative stress by producing reactive nitrogen species. Here we measured NO content by Griess method and the localization of inducible and endothelial nitric oxide synthase (iNOS and eNOS) by immunohistochemistry in implantation sites. Also we measured lipid peroxidation by TBA-RS, glutathion by Ellman’s reaction and antioxidant enzymes by enzymatic kinetics in uterine homogenates. We found that the expression of both iNOS and eNOS and the NO content were increased with DHEA (p<0.001 for all) and restored to control levels with DHEA+M. Oxidative stress: DHEA increased lipid peroxidation (p<0.01) and glutathione (GSH, p<0.01). With DHEA+M lipid peroxidation was restored to control levels. The activities of the antioxidant enzymes superoxide dismutase and catalase were not modified. We conclude that hyperandrogenization with DHEA enhances the NO system and lipid peroxidation in implantation sites of early pregnant mice and that M treatment prevents these effects.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20172
Luchetti, Carolina Griselda; Paz, Dante Agustin; Motta, Alicia Beatriz; Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice; Bentham Science Publishers; The Open Reproductive Science Journal; 7; 9-2013; 14-22
1874-2556
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20172
identifier_str_mv Luchetti, Carolina Griselda; Paz, Dante Agustin; Motta, Alicia Beatriz; Metformin prevents the increase of nitric oxide and lipid peroxidation induced by dehydroepiandrosterone in early pregnant mice; Bentham Science Publishers; The Open Reproductive Science Journal; 7; 9-2013; 14-22
1874-2556
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2174/1874255620130913002
info:eu-repo/semantics/altIdentifier/url/https://benthamopen.com/ABSTRACT/TORSJ-5-14
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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