A simple experiment to show photodynamic inactivation of bacteria on surfaces

Autores
Caminos, Daniel Alberto; Durantini, Edgardo Néstor
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
New suitable approaches were investigated to visualize the photodynamic inactivation (PDI) of bacteria immobilized on agar surfaces. The PDI capacities of a cationic [5,10,15,20-tetra(4-N,N,N-trimethylammoniumphenyl)porphyrin] and an anionic [5,10,15,20-tetra(4-sulphonatophenyl)porphyrin] photosensitizers were analyzed on a typical Gram-negative bacterium Escherichia coli following two procedures. In experiment I, the E. coli cells were grown as lawn on agar surface containing the sensitizers spread in a small area (10 nmol in ~0.6 cm2). After irradiation with visible light (10 min, 90 mW/cm2), no cells were grown in the area containing the cationic porphyrin. In experiment II, small colonies (~2 mm diameter) of E. coli on agar were treaded with a solution of sensitizer (10 nmol) and irradiated with visible light for 3 h. Overnight incubation at 37ºC shows that a growth delay of E. coli colonies treated with the cationic photosensitizer. In contrast, the anionic porphyrin did not produce appreciable photodamage. These experiments could be used in either an undergraduate project for natural science advance students or for postgraduate practical training course. This methodology illustrates the application of PDI to treat bacteria growing as localized foci of infection.
Fil: Caminos, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
Photodynamic inactivation
Porphyrin
Bacteria
sensitizer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242020
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A simple experiment to show photodynamic inactivation of bacteria on surfacesCaminos, Daniel AlbertoDurantini, Edgardo NéstorPhotodynamic inactivationPorphyrinBacteriasensitizerhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1New suitable approaches were investigated to visualize the photodynamic inactivation (PDI) of bacteria immobilized on agar surfaces. The PDI capacities of a cationic [5,10,15,20-tetra(4-N,N,N-trimethylammoniumphenyl)porphyrin] and an anionic [5,10,15,20-tetra(4-sulphonatophenyl)porphyrin] photosensitizers were analyzed on a typical Gram-negative bacterium Escherichia coli following two procedures. In experiment I, the E. coli cells were grown as lawn on agar surface containing the sensitizers spread in a small area (10 nmol in ~0.6 cm2). After irradiation with visible light (10 min, 90 mW/cm2), no cells were grown in the area containing the cationic porphyrin. In experiment II, small colonies (~2 mm diameter) of E. coli on agar were treaded with a solution of sensitizer (10 nmol) and irradiated with visible light for 3 h. Overnight incubation at 37ºC shows that a growth delay of E. coli colonies treated with the cationic photosensitizer. In contrast, the anionic porphyrin did not produce appreciable photodamage. These experiments could be used in either an undergraduate project for natural science advance students or for postgraduate practical training course. This methodology illustrates the application of PDI to treat bacteria growing as localized foci of infection.Fil: Caminos, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaJohn Wiley & Sons2007-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242020Caminos, Daniel Alberto; Durantini, Edgardo Néstor; A simple experiment to show photodynamic inactivation of bacteria on surfaces; John Wiley & Sons; Biochemistry and Molecular Biology Education; 35; 1; 8-2007; 64-691470-8175CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bmb.11info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.11info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:34Zoai:ri.conicet.gov.ar:11336/242020instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:34.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simple experiment to show photodynamic inactivation of bacteria on surfaces
title A simple experiment to show photodynamic inactivation of bacteria on surfaces
spellingShingle A simple experiment to show photodynamic inactivation of bacteria on surfaces
Caminos, Daniel Alberto
Photodynamic inactivation
Porphyrin
Bacteria
sensitizer
title_short A simple experiment to show photodynamic inactivation of bacteria on surfaces
title_full A simple experiment to show photodynamic inactivation of bacteria on surfaces
title_fullStr A simple experiment to show photodynamic inactivation of bacteria on surfaces
title_full_unstemmed A simple experiment to show photodynamic inactivation of bacteria on surfaces
title_sort A simple experiment to show photodynamic inactivation of bacteria on surfaces
dc.creator.none.fl_str_mv Caminos, Daniel Alberto
Durantini, Edgardo Néstor
author Caminos, Daniel Alberto
author_facet Caminos, Daniel Alberto
Durantini, Edgardo Néstor
author_role author
author2 Durantini, Edgardo Néstor
author2_role author
dc.subject.none.fl_str_mv Photodynamic inactivation
Porphyrin
Bacteria
sensitizer
topic Photodynamic inactivation
Porphyrin
Bacteria
sensitizer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv New suitable approaches were investigated to visualize the photodynamic inactivation (PDI) of bacteria immobilized on agar surfaces. The PDI capacities of a cationic [5,10,15,20-tetra(4-N,N,N-trimethylammoniumphenyl)porphyrin] and an anionic [5,10,15,20-tetra(4-sulphonatophenyl)porphyrin] photosensitizers were analyzed on a typical Gram-negative bacterium Escherichia coli following two procedures. In experiment I, the E. coli cells were grown as lawn on agar surface containing the sensitizers spread in a small area (10 nmol in ~0.6 cm2). After irradiation with visible light (10 min, 90 mW/cm2), no cells were grown in the area containing the cationic porphyrin. In experiment II, small colonies (~2 mm diameter) of E. coli on agar were treaded with a solution of sensitizer (10 nmol) and irradiated with visible light for 3 h. Overnight incubation at 37ºC shows that a growth delay of E. coli colonies treated with the cationic photosensitizer. In contrast, the anionic porphyrin did not produce appreciable photodamage. These experiments could be used in either an undergraduate project for natural science advance students or for postgraduate practical training course. This methodology illustrates the application of PDI to treat bacteria growing as localized foci of infection.
Fil: Caminos, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description New suitable approaches were investigated to visualize the photodynamic inactivation (PDI) of bacteria immobilized on agar surfaces. The PDI capacities of a cationic [5,10,15,20-tetra(4-N,N,N-trimethylammoniumphenyl)porphyrin] and an anionic [5,10,15,20-tetra(4-sulphonatophenyl)porphyrin] photosensitizers were analyzed on a typical Gram-negative bacterium Escherichia coli following two procedures. In experiment I, the E. coli cells were grown as lawn on agar surface containing the sensitizers spread in a small area (10 nmol in ~0.6 cm2). After irradiation with visible light (10 min, 90 mW/cm2), no cells were grown in the area containing the cationic porphyrin. In experiment II, small colonies (~2 mm diameter) of E. coli on agar were treaded with a solution of sensitizer (10 nmol) and irradiated with visible light for 3 h. Overnight incubation at 37ºC shows that a growth delay of E. coli colonies treated with the cationic photosensitizer. In contrast, the anionic porphyrin did not produce appreciable photodamage. These experiments could be used in either an undergraduate project for natural science advance students or for postgraduate practical training course. This methodology illustrates the application of PDI to treat bacteria growing as localized foci of infection.
publishDate 2007
dc.date.none.fl_str_mv 2007-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242020
Caminos, Daniel Alberto; Durantini, Edgardo Néstor; A simple experiment to show photodynamic inactivation of bacteria on surfaces; John Wiley & Sons; Biochemistry and Molecular Biology Education; 35; 1; 8-2007; 64-69
1470-8175
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242020
identifier_str_mv Caminos, Daniel Alberto; Durantini, Edgardo Néstor; A simple experiment to show photodynamic inactivation of bacteria on surfaces; John Wiley & Sons; Biochemistry and Molecular Biology Education; 35; 1; 8-2007; 64-69
1470-8175
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bmb.11
info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.11
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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