Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat

Autores
Vega Orozco, Adriana Soledad; Sosa, Zulema; Delgado, Silvia Marcela; Casais, Marilina; Rastrilla, Ana Maria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ovarian nervous plexus (ONP) is one of the principal extrinsic innervation pathways reaching the ovary from the superior mesenteric ganglion (SMG). The aims of this work were: (a) to determine if acetylcholine (Ach) in the SMG modifies the release of steroids and ovarian nitrites in an ex vivo SMG-ONP-ovary system on dioestrus (D) I and II, and (b) to demonstrate if the activities and gene expression of the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-hydroxysteroid dehydrogenase (20α-HSD) are modified by cholinergic stimulus. The system was incubated in Krebs-Ringer buffer bicarbonate at 37°C in metabolic bath. Ach (10-6M) was used as cholinergic agonist. Ach in SMG increased progesterone release at all the incubation times on DI and DII (p<0.001). Androstenedione increased at 15 and 30min on DI, and at 30min on DII whereas nitric oxide (NO) increased at 30min on DI, and at 15 and 30min on DII. The activity of 3β-HSD increased whereas the activity of 20α-HSD decreased (p<0.001) on DI and DII. The gene expression of 3β-HSD showed a significant increase at 120min on DI and DII (op<0.01) and 20α-HSD diminished only on DII. The results show the importance of the SMG via the ovarian nervous plexus on the regulation of the steroid secretory activity and on the ovarian release of NO in the luteal phase. The complex synaptic connections in the prevertebral ganglia and the sympathetic ganglionic chain participate in the neuroendocrinological mechanisms that take place during the luteal steroidogenesis.
Fil: Vega Orozco, Adriana Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sosa, Zulema. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Casais, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Materia
CHOLINERGIC AGENTS
OVARIAN NERVOUS PLEXUS
OVARY
STEROID
SUPERIOR MESENTERIC GANGLION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14588
Acceder
id CONICETDig_e52e04312105d407d51cd4164c31dffb
oai_identifier_str oai:ri.conicet.gov.ar:11336/14588
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in ratVega Orozco, Adriana SoledadSosa, ZulemaDelgado, Silvia MarcelaCasais, MarilinaRastrilla, Ana MariaCHOLINERGIC AGENTSOVARIAN NERVOUS PLEXUSOVARYSTEROIDSUPERIOR MESENTERIC GANGLIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The ovarian nervous plexus (ONP) is one of the principal extrinsic innervation pathways reaching the ovary from the superior mesenteric ganglion (SMG). The aims of this work were: (a) to determine if acetylcholine (Ach) in the SMG modifies the release of steroids and ovarian nitrites in an ex vivo SMG-ONP-ovary system on dioestrus (D) I and II, and (b) to demonstrate if the activities and gene expression of the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-hydroxysteroid dehydrogenase (20α-HSD) are modified by cholinergic stimulus. The system was incubated in Krebs-Ringer buffer bicarbonate at 37°C in metabolic bath. Ach (10-6M) was used as cholinergic agonist. Ach in SMG increased progesterone release at all the incubation times on DI and DII (p<0.001). Androstenedione increased at 15 and 30min on DI, and at 30min on DII whereas nitric oxide (NO) increased at 30min on DI, and at 15 and 30min on DII. The activity of 3β-HSD increased whereas the activity of 20α-HSD decreased (p<0.001) on DI and DII. The gene expression of 3β-HSD showed a significant increase at 120min on DI and DII (op<0.01) and 20α-HSD diminished only on DII. The results show the importance of the SMG via the ovarian nervous plexus on the regulation of the steroid secretory activity and on the ovarian release of NO in the luteal phase. The complex synaptic connections in the prevertebral ganglia and the sympathetic ganglionic chain participate in the neuroendocrinological mechanisms that take place during the luteal steroidogenesis.Fil: Vega Orozco, Adriana Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Sosa, Zulema. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Casais, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaPergamon-Elsevier Science Ltd2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14588Vega Orozco, Adriana Soledad; Sosa, Zulema; Delgado, Silvia Marcela; Casais, Marilina; Rastrilla, Ana Maria; Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 120; 1; 5-2010; 45-520960-0760enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076010001317info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2010.03.040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:53Zoai:ri.conicet.gov.ar:11336/14588instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:53.892CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
title Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
spellingShingle Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
Vega Orozco, Adriana Soledad
CHOLINERGIC AGENTS
OVARIAN NERVOUS PLEXUS
OVARY
STEROID
SUPERIOR MESENTERIC GANGLION
title_short Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
title_full Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
title_fullStr Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
title_full_unstemmed Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
title_sort Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat
dc.creator.none.fl_str_mv Vega Orozco, Adriana Soledad
Sosa, Zulema
Delgado, Silvia Marcela
Casais, Marilina
Rastrilla, Ana Maria
author Vega Orozco, Adriana Soledad
author_facet Vega Orozco, Adriana Soledad
Sosa, Zulema
Delgado, Silvia Marcela
Casais, Marilina
Rastrilla, Ana Maria
author_role author
author2 Sosa, Zulema
Delgado, Silvia Marcela
Casais, Marilina
Rastrilla, Ana Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHOLINERGIC AGENTS
OVARIAN NERVOUS PLEXUS
OVARY
STEROID
SUPERIOR MESENTERIC GANGLION
topic CHOLINERGIC AGENTS
OVARIAN NERVOUS PLEXUS
OVARY
STEROID
SUPERIOR MESENTERIC GANGLION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The ovarian nervous plexus (ONP) is one of the principal extrinsic innervation pathways reaching the ovary from the superior mesenteric ganglion (SMG). The aims of this work were: (a) to determine if acetylcholine (Ach) in the SMG modifies the release of steroids and ovarian nitrites in an ex vivo SMG-ONP-ovary system on dioestrus (D) I and II, and (b) to demonstrate if the activities and gene expression of the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-hydroxysteroid dehydrogenase (20α-HSD) are modified by cholinergic stimulus. The system was incubated in Krebs-Ringer buffer bicarbonate at 37°C in metabolic bath. Ach (10-6M) was used as cholinergic agonist. Ach in SMG increased progesterone release at all the incubation times on DI and DII (p<0.001). Androstenedione increased at 15 and 30min on DI, and at 30min on DII whereas nitric oxide (NO) increased at 30min on DI, and at 15 and 30min on DII. The activity of 3β-HSD increased whereas the activity of 20α-HSD decreased (p<0.001) on DI and DII. The gene expression of 3β-HSD showed a significant increase at 120min on DI and DII (op<0.01) and 20α-HSD diminished only on DII. The results show the importance of the SMG via the ovarian nervous plexus on the regulation of the steroid secretory activity and on the ovarian release of NO in the luteal phase. The complex synaptic connections in the prevertebral ganglia and the sympathetic ganglionic chain participate in the neuroendocrinological mechanisms that take place during the luteal steroidogenesis.
Fil: Vega Orozco, Adriana Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sosa, Zulema. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Casais, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
description The ovarian nervous plexus (ONP) is one of the principal extrinsic innervation pathways reaching the ovary from the superior mesenteric ganglion (SMG). The aims of this work were: (a) to determine if acetylcholine (Ach) in the SMG modifies the release of steroids and ovarian nitrites in an ex vivo SMG-ONP-ovary system on dioestrus (D) I and II, and (b) to demonstrate if the activities and gene expression of the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-hydroxysteroid dehydrogenase (20α-HSD) are modified by cholinergic stimulus. The system was incubated in Krebs-Ringer buffer bicarbonate at 37°C in metabolic bath. Ach (10-6M) was used as cholinergic agonist. Ach in SMG increased progesterone release at all the incubation times on DI and DII (p<0.001). Androstenedione increased at 15 and 30min on DI, and at 30min on DII whereas nitric oxide (NO) increased at 30min on DI, and at 15 and 30min on DII. The activity of 3β-HSD increased whereas the activity of 20α-HSD decreased (p<0.001) on DI and DII. The gene expression of 3β-HSD showed a significant increase at 120min on DI and DII (op<0.01) and 20α-HSD diminished only on DII. The results show the importance of the SMG via the ovarian nervous plexus on the regulation of the steroid secretory activity and on the ovarian release of NO in the luteal phase. The complex synaptic connections in the prevertebral ganglia and the sympathetic ganglionic chain participate in the neuroendocrinological mechanisms that take place during the luteal steroidogenesis.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14588
Vega Orozco, Adriana Soledad; Sosa, Zulema; Delgado, Silvia Marcela; Casais, Marilina; Rastrilla, Ana Maria; Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 120; 1; 5-2010; 45-52
0960-0760
url http://hdl.handle.net/11336/14588
identifier_str_mv Vega Orozco, Adriana Soledad; Sosa, Zulema; Delgado, Silvia Marcela; Casais, Marilina; Rastrilla, Ana Maria; Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 120; 1; 5-2010; 45-52
0960-0760
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076010001317
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2010.03.040
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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