Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve

Autores
Casais, Marilina; Vallcaneras, Sandra; Campo Verde Arbocco, Fiorella; Delgado, Silvia Marcela; Hapon, María Belén; Sosa, Zulema Yolanda; Telleria, Carlos M.; Rastrilla, Ana Maria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is evidence suggesting that estradiol (E2) regulates the physiology of the ovary and the sympathetic neurons associated with the reproductive function. The objective of this study was to investigate the effect of E2 on the function of late pregnant rat ovaries, acting either directly on the ovarian tissue or indirectly via the superior ovarian nerve (SON) from the celiac ganglion (CG). We used in vitro ovary (OV) or ex vivo CG-SON-OV incubation systems from day 21 pregnant rats. Various concentrations of E2 were added to the incubation media of either the OV alone or the ganglion compartment of the CG-SON-OV system. In both experimental schemes, we measured the concentration of progesterone in the OV incubation media by radioimmunoassay at different times. Luteal Messenger RNA (mRNA) expression of 3b hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD) enzymes, respectively, involved in progesterone synthesis and catabolism, and of antiapoptotic B-cell lymphoma 2 (Bcl-2) and proapoptotic Bcl-2-associated X protein (Bax), were measured by reverse transcriptase?polymerase chain reaction (RT-PCR) at the end of the incubation period. Estradiol added directly to the OV incubation or to the CG of the CG-SON-OV system caused a decline in the concentration of progesterone accumulated in the incubation media. In addition, E2, when added to the OV incubation, decreased the expression of 3b-HSD and the ratio of Bcl-2/Bax. We conclude that through a direct effect on the OV, E2 favors luteal regression at the end of pregnancy in rats, in association with neural modulation from the CG via the SON.
Fil: Casais, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Vallcaneras, Sandra. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Campo Verde Arbocco, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Telleria, Carlos M.. University of South Dakota; Estados Unidos
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Materia
ESTRADIOL
REGRESSION
OVARY
GANGLION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272792
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272792
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian NerveCasais, MarilinaVallcaneras, SandraCampo Verde Arbocco, FiorellaDelgado, Silvia MarcelaHapon, María BelénSosa, Zulema YolandaTelleria, Carlos M.Rastrilla, Ana MariaESTRADIOLREGRESSIONOVARYGANGLIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3There is evidence suggesting that estradiol (E2) regulates the physiology of the ovary and the sympathetic neurons associated with the reproductive function. The objective of this study was to investigate the effect of E2 on the function of late pregnant rat ovaries, acting either directly on the ovarian tissue or indirectly via the superior ovarian nerve (SON) from the celiac ganglion (CG). We used in vitro ovary (OV) or ex vivo CG-SON-OV incubation systems from day 21 pregnant rats. Various concentrations of E2 were added to the incubation media of either the OV alone or the ganglion compartment of the CG-SON-OV system. In both experimental schemes, we measured the concentration of progesterone in the OV incubation media by radioimmunoassay at different times. Luteal Messenger RNA (mRNA) expression of 3b hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD) enzymes, respectively, involved in progesterone synthesis and catabolism, and of antiapoptotic B-cell lymphoma 2 (Bcl-2) and proapoptotic Bcl-2-associated X protein (Bax), were measured by reverse transcriptase?polymerase chain reaction (RT-PCR) at the end of the incubation period. Estradiol added directly to the OV incubation or to the CG of the CG-SON-OV system caused a decline in the concentration of progesterone accumulated in the incubation media. In addition, E2, when added to the OV incubation, decreased the expression of 3b-HSD and the ratio of Bcl-2/Bax. We conclude that through a direct effect on the OV, E2 favors luteal regression at the end of pregnancy in rats, in association with neural modulation from the CG via the SON.Fil: Casais, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Vallcaneras, Sandra. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Campo Verde Arbocco, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaFil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Telleria, Carlos M.. University of South Dakota; Estados UnidosFil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaSAGE Publications2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272792Casais, Marilina; Vallcaneras, Sandra; Campo Verde Arbocco, Fiorella; Delgado, Silvia Marcela; Hapon, María Belén; et al.; Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve; SAGE Publications; Reproductive Sciences; 19; 4; 12-2012; 416-4221933-7191CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1933719111424436info:eu-repo/semantics/altIdentifier/doi/10.1177/1933719111424436info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:10:15Zoai:ri.conicet.gov.ar:11336/272792instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:10:16.114CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
title Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
spellingShingle Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
Casais, Marilina
ESTRADIOL
REGRESSION
OVARY
GANGLION
title_short Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
title_full Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
title_fullStr Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
title_full_unstemmed Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
title_sort Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve
dc.creator.none.fl_str_mv Casais, Marilina
Vallcaneras, Sandra
Campo Verde Arbocco, Fiorella
Delgado, Silvia Marcela
Hapon, María Belén
Sosa, Zulema Yolanda
Telleria, Carlos M.
Rastrilla, Ana Maria
author Casais, Marilina
author_facet Casais, Marilina
Vallcaneras, Sandra
Campo Verde Arbocco, Fiorella
Delgado, Silvia Marcela
Hapon, María Belén
Sosa, Zulema Yolanda
Telleria, Carlos M.
Rastrilla, Ana Maria
author_role author
author2 Vallcaneras, Sandra
Campo Verde Arbocco, Fiorella
Delgado, Silvia Marcela
Hapon, María Belén
Sosa, Zulema Yolanda
Telleria, Carlos M.
Rastrilla, Ana Maria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESTRADIOL
REGRESSION
OVARY
GANGLION
topic ESTRADIOL
REGRESSION
OVARY
GANGLION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv There is evidence suggesting that estradiol (E2) regulates the physiology of the ovary and the sympathetic neurons associated with the reproductive function. The objective of this study was to investigate the effect of E2 on the function of late pregnant rat ovaries, acting either directly on the ovarian tissue or indirectly via the superior ovarian nerve (SON) from the celiac ganglion (CG). We used in vitro ovary (OV) or ex vivo CG-SON-OV incubation systems from day 21 pregnant rats. Various concentrations of E2 were added to the incubation media of either the OV alone or the ganglion compartment of the CG-SON-OV system. In both experimental schemes, we measured the concentration of progesterone in the OV incubation media by radioimmunoassay at different times. Luteal Messenger RNA (mRNA) expression of 3b hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD) enzymes, respectively, involved in progesterone synthesis and catabolism, and of antiapoptotic B-cell lymphoma 2 (Bcl-2) and proapoptotic Bcl-2-associated X protein (Bax), were measured by reverse transcriptase?polymerase chain reaction (RT-PCR) at the end of the incubation period. Estradiol added directly to the OV incubation or to the CG of the CG-SON-OV system caused a decline in the concentration of progesterone accumulated in the incubation media. In addition, E2, when added to the OV incubation, decreased the expression of 3b-HSD and the ratio of Bcl-2/Bax. We conclude that through a direct effect on the OV, E2 favors luteal regression at the end of pregnancy in rats, in association with neural modulation from the CG via the SON.
Fil: Casais, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Vallcaneras, Sandra. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Campo Verde Arbocco, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Telleria, Carlos M.. University of South Dakota; Estados Unidos
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
description There is evidence suggesting that estradiol (E2) regulates the physiology of the ovary and the sympathetic neurons associated with the reproductive function. The objective of this study was to investigate the effect of E2 on the function of late pregnant rat ovaries, acting either directly on the ovarian tissue or indirectly via the superior ovarian nerve (SON) from the celiac ganglion (CG). We used in vitro ovary (OV) or ex vivo CG-SON-OV incubation systems from day 21 pregnant rats. Various concentrations of E2 were added to the incubation media of either the OV alone or the ganglion compartment of the CG-SON-OV system. In both experimental schemes, we measured the concentration of progesterone in the OV incubation media by radioimmunoassay at different times. Luteal Messenger RNA (mRNA) expression of 3b hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD) enzymes, respectively, involved in progesterone synthesis and catabolism, and of antiapoptotic B-cell lymphoma 2 (Bcl-2) and proapoptotic Bcl-2-associated X protein (Bax), were measured by reverse transcriptase?polymerase chain reaction (RT-PCR) at the end of the incubation period. Estradiol added directly to the OV incubation or to the CG of the CG-SON-OV system caused a decline in the concentration of progesterone accumulated in the incubation media. In addition, E2, when added to the OV incubation, decreased the expression of 3b-HSD and the ratio of Bcl-2/Bax. We conclude that through a direct effect on the OV, E2 favors luteal regression at the end of pregnancy in rats, in association with neural modulation from the CG via the SON.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272792
Casais, Marilina; Vallcaneras, Sandra; Campo Verde Arbocco, Fiorella; Delgado, Silvia Marcela; Hapon, María Belén; et al.; Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve; SAGE Publications; Reproductive Sciences; 19; 4; 12-2012; 416-422
1933-7191
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272792
identifier_str_mv Casais, Marilina; Vallcaneras, Sandra; Campo Verde Arbocco, Fiorella; Delgado, Silvia Marcela; Hapon, María Belén; et al.; Estradiol Promotes Luteal Regression Through a Direct Effect on the Ovary and an Indirect Effect From the Celiac Ganglion via the Superior Ovarian Nerve; SAGE Publications; Reproductive Sciences; 19; 4; 12-2012; 416-422
1933-7191
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1933719111424436
info:eu-repo/semantics/altIdentifier/doi/10.1177/1933719111424436
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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