Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo

Autores
Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; Papadopoulos, Alexia; Pasquevich, Karina Alejandra; Méresse, Stéphane; Cassataro, Juliana; Mémet, Sylvie; Gorvel, Jean Pierre
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.
Fil: Degos, Clara. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Hysenaj, Lisiena. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gonzalez Espinoza, Gabriela. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Arce Gorvel, Vilma. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gagnaire, Aurélie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Papadopoulos, Alexia. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Pasquevich, Karina Alejandra. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Méresse, Stéphane. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mémet, Sylvie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gorvel, Jean Pierre. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Materia
ACUTE AND CHRONIC INFECTION
BRUCELLA
CD150
DENDRITIC CELLS
INFLAMMATION
NF-ΚB
OMP25
SLAMF1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140371
Acceder
id CONICETDig_e5138e9c632e5c830fb53678e24c0f38
oai_identifier_str oai:ri.conicet.gov.ar:11336/140371
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivoDegos, ClaraHysenaj, LisienaGonzalez Espinoza, GabrielaArce Gorvel, VilmaGagnaire, AuréliePapadopoulos, AlexiaPasquevich, Karina AlejandraMéresse, StéphaneCassataro, JulianaMémet, SylvieGorvel, Jean PierreACUTE AND CHRONIC INFECTIONBRUCELLACD150DENDRITIC CELLSINFLAMMATIONNF-ΚBOMP25SLAMF1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.Fil: Degos, Clara. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Hysenaj, Lisiena. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gonzalez Espinoza, Gabriela. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Arce Gorvel, Vilma. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gagnaire, Aurélie. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Papadopoulos, Alexia. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Pasquevich, Karina Alejandra. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Méresse, Stéphane. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Mémet, Sylvie. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gorvel, Jean Pierre. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaWiley Blackwell Publishing, Inc2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140371Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; et al.; Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 4; 1-2020; 1-481462-5814CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13164info:eu-repo/semantics/altIdentifier/doi/10.1111/cmi.13164info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:27Zoai:ri.conicet.gov.ar:11336/140371instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:27.54CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
title Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
spellingShingle Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
Degos, Clara
ACUTE AND CHRONIC INFECTION
BRUCELLA
CD150
DENDRITIC CELLS
INFLAMMATION
NF-ΚB
OMP25
SLAMF1
title_short Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
title_full Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
title_fullStr Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
title_full_unstemmed Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
title_sort Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
dc.creator.none.fl_str_mv Degos, Clara
Hysenaj, Lisiena
Gonzalez Espinoza, Gabriela
Arce Gorvel, Vilma
Gagnaire, Aurélie
Papadopoulos, Alexia
Pasquevich, Karina Alejandra
Méresse, Stéphane
Cassataro, Juliana
Mémet, Sylvie
Gorvel, Jean Pierre
author Degos, Clara
author_facet Degos, Clara
Hysenaj, Lisiena
Gonzalez Espinoza, Gabriela
Arce Gorvel, Vilma
Gagnaire, Aurélie
Papadopoulos, Alexia
Pasquevich, Karina Alejandra
Méresse, Stéphane
Cassataro, Juliana
Mémet, Sylvie
Gorvel, Jean Pierre
author_role author
author2 Hysenaj, Lisiena
Gonzalez Espinoza, Gabriela
Arce Gorvel, Vilma
Gagnaire, Aurélie
Papadopoulos, Alexia
Pasquevich, Karina Alejandra
Méresse, Stéphane
Cassataro, Juliana
Mémet, Sylvie
Gorvel, Jean Pierre
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACUTE AND CHRONIC INFECTION
BRUCELLA
CD150
DENDRITIC CELLS
INFLAMMATION
NF-ΚB
OMP25
SLAMF1
topic ACUTE AND CHRONIC INFECTION
BRUCELLA
CD150
DENDRITIC CELLS
INFLAMMATION
NF-ΚB
OMP25
SLAMF1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.
Fil: Degos, Clara. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Hysenaj, Lisiena. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gonzalez Espinoza, Gabriela. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Arce Gorvel, Vilma. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gagnaire, Aurélie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Papadopoulos, Alexia. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Pasquevich, Karina Alejandra. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Méresse, Stéphane. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mémet, Sylvie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gorvel, Jean Pierre. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
description The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140371
Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; et al.; Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 4; 1-2020; 1-48
1462-5814
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140371
identifier_str_mv Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; et al.; Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 4; 1-2020; 1-48
1462-5814
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13164
info:eu-repo/semantics/altIdentifier/doi/10.1111/cmi.13164
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781842546491392
score 12.982451

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