Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo
- Autores
- Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; Papadopoulos, Alexia; Pasquevich, Karina Alejandra; Méresse, Stéphane; Cassataro, Juliana; Mémet, Sylvie; Gorvel, Jean Pierre
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.
Fil: Degos, Clara. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Hysenaj, Lisiena. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gonzalez Espinoza, Gabriela. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Arce Gorvel, Vilma. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gagnaire, Aurélie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Papadopoulos, Alexia. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Pasquevich, Karina Alejandra. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Méresse, Stéphane. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mémet, Sylvie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gorvel, Jean Pierre. Inserm; Francia. Centre National de la Recherche Scientifique; Francia - Materia
-
ACUTE AND CHRONIC INFECTION
BRUCELLA
CD150
DENDRITIC CELLS
INFLAMMATION
NF-ΚB
OMP25
SLAMF1 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/140371
Ver los metadatos del registro completo
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Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivoDegos, ClaraHysenaj, LisienaGonzalez Espinoza, GabrielaArce Gorvel, VilmaGagnaire, AuréliePapadopoulos, AlexiaPasquevich, Karina AlejandraMéresse, StéphaneCassataro, JulianaMémet, SylvieGorvel, Jean PierreACUTE AND CHRONIC INFECTIONBRUCELLACD150DENDRITIC CELLSINFLAMMATIONNF-ΚBOMP25SLAMF1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity.Fil: Degos, Clara. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Hysenaj, Lisiena. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gonzalez Espinoza, Gabriela. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Arce Gorvel, Vilma. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gagnaire, Aurélie. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Papadopoulos, Alexia. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Pasquevich, Karina Alejandra. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Méresse, Stéphane. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Mémet, Sylvie. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gorvel, Jean Pierre. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaWiley Blackwell Publishing, Inc2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140371Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; et al.; Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 4; 1-2020; 1-481462-5814CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13164info:eu-repo/semantics/altIdentifier/doi/10.1111/cmi.13164info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:08Zoai:ri.conicet.gov.ar:11336/140371instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:08.594CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
title |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
spellingShingle |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo Degos, Clara ACUTE AND CHRONIC INFECTION BRUCELLA CD150 DENDRITIC CELLS INFLAMMATION NF-ΚB OMP25 SLAMF1 |
title_short |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
title_full |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
title_fullStr |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
title_full_unstemmed |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
title_sort |
Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo |
dc.creator.none.fl_str_mv |
Degos, Clara Hysenaj, Lisiena Gonzalez Espinoza, Gabriela Arce Gorvel, Vilma Gagnaire, Aurélie Papadopoulos, Alexia Pasquevich, Karina Alejandra Méresse, Stéphane Cassataro, Juliana Mémet, Sylvie Gorvel, Jean Pierre |
author |
Degos, Clara |
author_facet |
Degos, Clara Hysenaj, Lisiena Gonzalez Espinoza, Gabriela Arce Gorvel, Vilma Gagnaire, Aurélie Papadopoulos, Alexia Pasquevich, Karina Alejandra Méresse, Stéphane Cassataro, Juliana Mémet, Sylvie Gorvel, Jean Pierre |
author_role |
author |
author2 |
Hysenaj, Lisiena Gonzalez Espinoza, Gabriela Arce Gorvel, Vilma Gagnaire, Aurélie Papadopoulos, Alexia Pasquevich, Karina Alejandra Méresse, Stéphane Cassataro, Juliana Mémet, Sylvie Gorvel, Jean Pierre |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ACUTE AND CHRONIC INFECTION BRUCELLA CD150 DENDRITIC CELLS INFLAMMATION NF-ΚB OMP25 SLAMF1 |
topic |
ACUTE AND CHRONIC INFECTION BRUCELLA CD150 DENDRITIC CELLS INFLAMMATION NF-ΚB OMP25 SLAMF1 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity. Fil: Degos, Clara. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Hysenaj, Lisiena. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Gonzalez Espinoza, Gabriela. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Arce Gorvel, Vilma. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Gagnaire, Aurélie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Papadopoulos, Alexia. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Pasquevich, Karina Alejandra. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Méresse, Stéphane. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Mémet, Sylvie. Inserm; Francia. Centre National de la Recherche Scientifique; Francia Fil: Gorvel, Jean Pierre. Inserm; Francia. Centre National de la Recherche Scientifique; Francia |
description |
The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25-dependent engagement of SLAMF1 by B. abortus limits NF-κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro-inflammatory cytokine secretion and impairs DC activation. The Omp25-SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/140371 Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; et al.; Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 4; 1-2020; 1-48 1462-5814 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/140371 |
identifier_str_mv |
Degos, Clara; Hysenaj, Lisiena; Gonzalez Espinoza, Gabriela; Arce Gorvel, Vilma; Gagnaire, Aurélie; et al.; Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo; Wiley Blackwell Publishing, Inc; Cellular Microbiology (print); 22; 4; 1-2020; 1-48 1462-5814 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13164 info:eu-repo/semantics/altIdentifier/doi/10.1111/cmi.13164 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613734840926208 |
score |
13.070432 |