Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane

Autores
Buschiazzo, Jorgelina; Ríos, G. L.; Canizo, Jesica Romina; Antollini, Silvia Susana; Alberio, R.H.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Methyl-β-cyclodextrin (MβCD) saturated with cholesterol or alone was used to modulate plasma membrane cholesterol level of metaphase II bovine oocytes. Membrane biophysical state of oocytes and cumulus cells was evaluated at decreasing temperatures and changes in the localization of the raft marker GM1 were analyzed in Cryotop vitrified oocytes. After 2 hours of incubation with 15 mM MβCD saturated with cholesterol, fluorescence intensity of BODIPY-cholesterol at the plasma membrane increased. Oocytes that were incubated with MβCD alone showed no differences in membrane fluorescence intensity but evidenced changes in the distribution of cytoplasmic lipid droplets. Laurdan generalized polarization revealed that membrane fluidity of oocytes is lower than that of cumulus cells at temperatures closer to the physiological but this relation is inverted when temperature decreases below 20°C. GM1 loss caused by vitrification was restored when cholesterol was incorporated into oocytes before vitrification and removed after warming, thus indicating that membrane raft integrity can be preserved by cholesterol modulation.
Fil: Buschiazzo, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ríos, G. L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Canizo, Jesica Romina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Alberio, R.H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XV Jornadas Anuales de la Sociedad Argentina de Biología
Argentina
Sociedad Argentina de Biología
Materia
Bovine oocytes
Cholesterol
Vitrification
Membrane fluidity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/233502
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membraneBuschiazzo, JorgelinaRíos, G. L.Canizo, Jesica RominaAntollini, Silvia SusanaAlberio, R.H.Bovine oocytesCholesterolVitrificationMembrane fluidityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Methyl-β-cyclodextrin (MβCD) saturated with cholesterol or alone was used to modulate plasma membrane cholesterol level of metaphase II bovine oocytes. Membrane biophysical state of oocytes and cumulus cells was evaluated at decreasing temperatures and changes in the localization of the raft marker GM1 were analyzed in Cryotop vitrified oocytes. After 2 hours of incubation with 15 mM MβCD saturated with cholesterol, fluorescence intensity of BODIPY-cholesterol at the plasma membrane increased. Oocytes that were incubated with MβCD alone showed no differences in membrane fluorescence intensity but evidenced changes in the distribution of cytoplasmic lipid droplets. Laurdan generalized polarization revealed that membrane fluidity of oocytes is lower than that of cumulus cells at temperatures closer to the physiological but this relation is inverted when temperature decreases below 20°C. GM1 loss caused by vitrification was restored when cholesterol was incorporated into oocytes before vitrification and removed after warming, thus indicating that membrane raft integrity can be preserved by cholesterol modulation.Fil: Buschiazzo, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Ríos, G. L.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Canizo, Jesica Romina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Alberio, R.H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXV Jornadas Anuales de la Sociedad Argentina de BiologíaArgentinaSociedad Argentina de BiologíaTech Science Press2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/233502Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane; XV Jornadas Anuales de la Sociedad Argentina de Biología; Argentina; 2013; 27-280327-95451669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34068/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:08Zoai:ri.conicet.gov.ar:11336/233502instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:08.848CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
title Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
spellingShingle Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
Buschiazzo, Jorgelina
Bovine oocytes
Cholesterol
Vitrification
Membrane fluidity
title_short Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
title_full Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
title_fullStr Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
title_full_unstemmed Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
title_sort Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane
dc.creator.none.fl_str_mv Buschiazzo, Jorgelina
Ríos, G. L.
Canizo, Jesica Romina
Antollini, Silvia Susana
Alberio, R.H.
author Buschiazzo, Jorgelina
author_facet Buschiazzo, Jorgelina
Ríos, G. L.
Canizo, Jesica Romina
Antollini, Silvia Susana
Alberio, R.H.
author_role author
author2 Ríos, G. L.
Canizo, Jesica Romina
Antollini, Silvia Susana
Alberio, R.H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine oocytes
Cholesterol
Vitrification
Membrane fluidity
topic Bovine oocytes
Cholesterol
Vitrification
Membrane fluidity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Methyl-β-cyclodextrin (MβCD) saturated with cholesterol or alone was used to modulate plasma membrane cholesterol level of metaphase II bovine oocytes. Membrane biophysical state of oocytes and cumulus cells was evaluated at decreasing temperatures and changes in the localization of the raft marker GM1 were analyzed in Cryotop vitrified oocytes. After 2 hours of incubation with 15 mM MβCD saturated with cholesterol, fluorescence intensity of BODIPY-cholesterol at the plasma membrane increased. Oocytes that were incubated with MβCD alone showed no differences in membrane fluorescence intensity but evidenced changes in the distribution of cytoplasmic lipid droplets. Laurdan generalized polarization revealed that membrane fluidity of oocytes is lower than that of cumulus cells at temperatures closer to the physiological but this relation is inverted when temperature decreases below 20°C. GM1 loss caused by vitrification was restored when cholesterol was incorporated into oocytes before vitrification and removed after warming, thus indicating that membrane raft integrity can be preserved by cholesterol modulation.
Fil: Buschiazzo, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ríos, G. L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Canizo, Jesica Romina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Alberio, R.H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XV Jornadas Anuales de la Sociedad Argentina de Biología
Argentina
Sociedad Argentina de Biología
description Methyl-β-cyclodextrin (MβCD) saturated with cholesterol or alone was used to modulate plasma membrane cholesterol level of metaphase II bovine oocytes. Membrane biophysical state of oocytes and cumulus cells was evaluated at decreasing temperatures and changes in the localization of the raft marker GM1 were analyzed in Cryotop vitrified oocytes. After 2 hours of incubation with 15 mM MβCD saturated with cholesterol, fluorescence intensity of BODIPY-cholesterol at the plasma membrane increased. Oocytes that were incubated with MβCD alone showed no differences in membrane fluorescence intensity but evidenced changes in the distribution of cytoplasmic lipid droplets. Laurdan generalized polarization revealed that membrane fluidity of oocytes is lower than that of cumulus cells at temperatures closer to the physiological but this relation is inverted when temperature decreases below 20°C. GM1 loss caused by vitrification was restored when cholesterol was incorporated into oocytes before vitrification and removed after warming, thus indicating that membrane raft integrity can be preserved by cholesterol modulation.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/233502
Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane; XV Jornadas Anuales de la Sociedad Argentina de Biología; Argentina; 2013; 27-28
0327-9545
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/233502
identifier_str_mv Cholesterol incorporation into bovine oocytes prior to vitrification favors recovery of GM1 level at the plasma membrane; XV Jornadas Anuales de la Sociedad Argentina de Biología; Argentina; 2013; 27-28
0327-9545
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v38nSuppl.S/34068/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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