Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures
- Autores
- Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Castellanos, Lucia Ines; Siñeriz, Faustino
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The present study explored the ability of the yeast Rhodotorula mucilaginosa RCL-11 to adapt to increasing Cu(II) concentrations, measuring oxidative stress through superoxide dismutase and catalase activity in two parallel sequential batch assays. One assay was performed in Erlenmeyer flasks without aeration and a second in a fermenter in which the dissolved oxygen was maintained at 30% saturation. Both assays were carried out by increasing Cu(II) concentrations in five sequential steps: 0; 0.1; 0.2; 0.5 and 1 mM. Each assay was incubated at 30°C, 250 rpm and pH 5.5. While growth parameters of R. mucilaginosa RCL-11 decreased 90-95% with increasing Cu(II) concentration in the culture medium, the oxidative stress level increased from 30 to 55% in both assays. Cells grown under controlled oxygen conditions showed 30% more copper bioaccumulation and 10% glucose consumption when compared with cells grown without aeration. SOD activity was higher under controlled than whitout areation, whereas CAT activity was similar under both test conditions. Cu(II) bioaccumulation by R. mucilaginosa RCL-11 and a possible increase in this capacity by adaptation of the strain under controlled aeration represent a potential valuable tool for treatment of effluents or water bioremediation with high copper contents.
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina - Materia
-
Copper(Ii) Uptake
Oxidative Stress
Rhodotorula Mucilaginosa
Sequential Batch - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/65936
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch culturesVillegas, Liliana BeatrizAmoroso, Maria Julia del R.Castellanos, Lucia InesSiñeriz, FaustinoCopper(Ii) UptakeOxidative StressRhodotorula MucilaginosaSequential Batchhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present study explored the ability of the yeast Rhodotorula mucilaginosa RCL-11 to adapt to increasing Cu(II) concentrations, measuring oxidative stress through superoxide dismutase and catalase activity in two parallel sequential batch assays. One assay was performed in Erlenmeyer flasks without aeration and a second in a fermenter in which the dissolved oxygen was maintained at 30% saturation. Both assays were carried out by increasing Cu(II) concentrations in five sequential steps: 0; 0.1; 0.2; 0.5 and 1 mM. Each assay was incubated at 30°C, 250 rpm and pH 5.5. While growth parameters of R. mucilaginosa RCL-11 decreased 90-95% with increasing Cu(II) concentration in the culture medium, the oxidative stress level increased from 30 to 55% in both assays. Cells grown under controlled oxygen conditions showed 30% more copper bioaccumulation and 10% glucose consumption when compared with cells grown without aeration. SOD activity was higher under controlled than whitout areation, whereas CAT activity was similar under both test conditions. Cu(II) bioaccumulation by R. mucilaginosa RCL-11 and a possible increase in this capacity by adaptation of the strain under controlled aeration represent a potential valuable tool for treatment of effluents or water bioremediation with high copper contents.Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaIWA Publishing2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65936Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Castellanos, Lucia Ines; Siñeriz, Faustino; Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures; IWA Publishing; Water Science And Technology; 60; 5; 9-2009; 1225-12320273-1223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2009.457info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article/60/5/1225/14508/Cu-II-removal-by-Rhodotorula-mucilaginosa-RCL-11info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:33Zoai:ri.conicet.gov.ar:11336/65936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:34.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
title |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
spellingShingle |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures Villegas, Liliana Beatriz Copper(Ii) Uptake Oxidative Stress Rhodotorula Mucilaginosa Sequential Batch |
title_short |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
title_full |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
title_fullStr |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
title_full_unstemmed |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
title_sort |
Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures |
dc.creator.none.fl_str_mv |
Villegas, Liliana Beatriz Amoroso, Maria Julia del R. Castellanos, Lucia Ines Siñeriz, Faustino |
author |
Villegas, Liliana Beatriz |
author_facet |
Villegas, Liliana Beatriz Amoroso, Maria Julia del R. Castellanos, Lucia Ines Siñeriz, Faustino |
author_role |
author |
author2 |
Amoroso, Maria Julia del R. Castellanos, Lucia Ines Siñeriz, Faustino |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Copper(Ii) Uptake Oxidative Stress Rhodotorula Mucilaginosa Sequential Batch |
topic |
Copper(Ii) Uptake Oxidative Stress Rhodotorula Mucilaginosa Sequential Batch |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The present study explored the ability of the yeast Rhodotorula mucilaginosa RCL-11 to adapt to increasing Cu(II) concentrations, measuring oxidative stress through superoxide dismutase and catalase activity in two parallel sequential batch assays. One assay was performed in Erlenmeyer flasks without aeration and a second in a fermenter in which the dissolved oxygen was maintained at 30% saturation. Both assays were carried out by increasing Cu(II) concentrations in five sequential steps: 0; 0.1; 0.2; 0.5 and 1 mM. Each assay was incubated at 30°C, 250 rpm and pH 5.5. While growth parameters of R. mucilaginosa RCL-11 decreased 90-95% with increasing Cu(II) concentration in the culture medium, the oxidative stress level increased from 30 to 55% in both assays. Cells grown under controlled oxygen conditions showed 30% more copper bioaccumulation and 10% glucose consumption when compared with cells grown without aeration. SOD activity was higher under controlled than whitout areation, whereas CAT activity was similar under both test conditions. Cu(II) bioaccumulation by R. mucilaginosa RCL-11 and a possible increase in this capacity by adaptation of the strain under controlled aeration represent a potential valuable tool for treatment of effluents or water bioremediation with high copper contents. Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina |
description |
The present study explored the ability of the yeast Rhodotorula mucilaginosa RCL-11 to adapt to increasing Cu(II) concentrations, measuring oxidative stress through superoxide dismutase and catalase activity in two parallel sequential batch assays. One assay was performed in Erlenmeyer flasks without aeration and a second in a fermenter in which the dissolved oxygen was maintained at 30% saturation. Both assays were carried out by increasing Cu(II) concentrations in five sequential steps: 0; 0.1; 0.2; 0.5 and 1 mM. Each assay was incubated at 30°C, 250 rpm and pH 5.5. While growth parameters of R. mucilaginosa RCL-11 decreased 90-95% with increasing Cu(II) concentration in the culture medium, the oxidative stress level increased from 30 to 55% in both assays. Cells grown under controlled oxygen conditions showed 30% more copper bioaccumulation and 10% glucose consumption when compared with cells grown without aeration. SOD activity was higher under controlled than whitout areation, whereas CAT activity was similar under both test conditions. Cu(II) bioaccumulation by R. mucilaginosa RCL-11 and a possible increase in this capacity by adaptation of the strain under controlled aeration represent a potential valuable tool for treatment of effluents or water bioremediation with high copper contents. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/65936 Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Castellanos, Lucia Ines; Siñeriz, Faustino; Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures; IWA Publishing; Water Science And Technology; 60; 5; 9-2009; 1225-1232 0273-1223 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/65936 |
identifier_str_mv |
Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Castellanos, Lucia Ines; Siñeriz, Faustino; Cu(II) removal by Rhodotorula mucilaginosa RCL-11 in sequential batch cultures; IWA Publishing; Water Science And Technology; 60; 5; 9-2009; 1225-1232 0273-1223 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.2166/wst.2009.457 info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/wst/article/60/5/1225/14508/Cu-II-removal-by-Rhodotorula-mucilaginosa-RCL-11 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
IWA Publishing |
publisher.none.fl_str_mv |
IWA Publishing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268981039202304 |
score |
13.13397 |