Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa

Autores
Alfaro, Juan Manuel; Reyes, Sarita Isabel; Martearena, Maria Rita; Blanco, Silvia Cristina; Baigori, Mario Domingo; Pera, Licia Maria
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The cell wall is a complex and dynamic structure. It is also a site of diverse enzyme activities. The balance between its synthesis and lysis is critical, since wall synthesis in the absence of lysis can cause excessive wall thickening and possible growth arrest; while, lysis in the absence of synthesis would produce bursting of the cell. Therefore, within permissible limits, the net balance between wall synthesis and wall lysis will exert a marked influence on the cell growth and morphology. In this work, the b-D-glucosidase activity was firstly selected as a marker of the wall lytic potential of both Rhodotorula glutinis and Rhodotorula mucilaginosa using p-nitrophenyl-β-D-glucopyranoside as substrate. Secondly, a doses-response study involving several metal ions was conducted; as a result, the FeCl3 and MnCl2 significant increased the biomass-bound β-D-glucosidase activity from R. glutinis and R. mucilaginosa, respectively. Thirdly, to test the influence of each metal ion, 6 mM of either FeCl3 (for R. mucilaginosa) or MnCl2 (for R. glutinis) was supplemented to a medium with lower nitrogen content (MLN). The MLN without any addition was taken as the control. Cultures were incubated at 30°C and 180 rpm during six days. Thus, the major and minor diameter of R. mucilaginosa increased from 3.40 ± 0.23 μm to 5.28 ± 0.40 μm and from 2.60 ± 0.23 μm to 5.20 ± 0.55 μm, respectively, in the presence of 6 mM FeCl3. And, the major and minor diameter of R. glutinis increased from 4,90 ± 0.47 μm to 5.48 ± 0.42 μm and from 2.23 ± 0.53 μm to 4.95 ± 0.49 μm, respectively, in the presence of 6 mM MnCl2. So, from these results it might be concluded that the supplementation of either FeCl3 or MnCl2 to the culture medium induced morphological changes in both oleaginous yeasts. In addition, the biomass and lipid concentration obtained after fermentations were also discussed.
Fil: Alfaro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Reyes, Sarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Martearena, Maria Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Blanco, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XII Congreso Argentino de Microbiología General
San Miguel de Tucumán
Argentina
Asociación Civil de Microbiología General
Materia
RHODOTORULA GLUTINIS
RHODOTORULA MUCILAGINOSA
METAL IONS
GLUCOSIDASE
CELL MORPHOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/188205
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosaAlfaro, Juan ManuelReyes, Sarita IsabelMartearena, Maria RitaBlanco, Silvia CristinaBaigori, Mario DomingoPera, Licia MariaRHODOTORULA GLUTINISRHODOTORULA MUCILAGINOSAMETAL IONSGLUCOSIDASECELL MORPHOLOGYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The cell wall is a complex and dynamic structure. It is also a site of diverse enzyme activities. The balance between its synthesis and lysis is critical, since wall synthesis in the absence of lysis can cause excessive wall thickening and possible growth arrest; while, lysis in the absence of synthesis would produce bursting of the cell. Therefore, within permissible limits, the net balance between wall synthesis and wall lysis will exert a marked influence on the cell growth and morphology. In this work, the b-D-glucosidase activity was firstly selected as a marker of the wall lytic potential of both Rhodotorula glutinis and Rhodotorula mucilaginosa using p-nitrophenyl-β-D-glucopyranoside as substrate. Secondly, a doses-response study involving several metal ions was conducted; as a result, the FeCl3 and MnCl2 significant increased the biomass-bound β-D-glucosidase activity from R. glutinis and R. mucilaginosa, respectively. Thirdly, to test the influence of each metal ion, 6 mM of either FeCl3 (for R. mucilaginosa) or MnCl2 (for R. glutinis) was supplemented to a medium with lower nitrogen content (MLN). The MLN without any addition was taken as the control. Cultures were incubated at 30°C and 180 rpm during six days. Thus, the major and minor diameter of R. mucilaginosa increased from 3.40 ± 0.23 μm to 5.28 ± 0.40 μm and from 2.60 ± 0.23 μm to 5.20 ± 0.55 μm, respectively, in the presence of 6 mM FeCl3. And, the major and minor diameter of R. glutinis increased from 4,90 ± 0.47 μm to 5.48 ± 0.42 μm and from 2.23 ± 0.53 μm to 4.95 ± 0.49 μm, respectively, in the presence of 6 mM MnCl2. So, from these results it might be concluded that the supplementation of either FeCl3 or MnCl2 to the culture medium induced morphological changes in both oleaginous yeasts. In addition, the biomass and lipid concentration obtained after fermentations were also discussed.Fil: Alfaro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Reyes, Sarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Martearena, Maria Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Blanco, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXII Congreso Argentino de Microbiología GeneralSan Miguel de TucumánArgentinaAsociación Civil de Microbiología GeneralAsociación Civil de Microbiología General2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/188205Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa; XII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2017; 1-4CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2017.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:53Zoai:ri.conicet.gov.ar:11336/188205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:53.469CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
title Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
spellingShingle Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
Alfaro, Juan Manuel
RHODOTORULA GLUTINIS
RHODOTORULA MUCILAGINOSA
METAL IONS
GLUCOSIDASE
CELL MORPHOLOGY
title_short Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
title_full Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
title_fullStr Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
title_full_unstemmed Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
title_sort Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa
dc.creator.none.fl_str_mv Alfaro, Juan Manuel
Reyes, Sarita Isabel
Martearena, Maria Rita
Blanco, Silvia Cristina
Baigori, Mario Domingo
Pera, Licia Maria
author Alfaro, Juan Manuel
author_facet Alfaro, Juan Manuel
Reyes, Sarita Isabel
Martearena, Maria Rita
Blanco, Silvia Cristina
Baigori, Mario Domingo
Pera, Licia Maria
author_role author
author2 Reyes, Sarita Isabel
Martearena, Maria Rita
Blanco, Silvia Cristina
Baigori, Mario Domingo
Pera, Licia Maria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv RHODOTORULA GLUTINIS
RHODOTORULA MUCILAGINOSA
METAL IONS
GLUCOSIDASE
CELL MORPHOLOGY
topic RHODOTORULA GLUTINIS
RHODOTORULA MUCILAGINOSA
METAL IONS
GLUCOSIDASE
CELL MORPHOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The cell wall is a complex and dynamic structure. It is also a site of diverse enzyme activities. The balance between its synthesis and lysis is critical, since wall synthesis in the absence of lysis can cause excessive wall thickening and possible growth arrest; while, lysis in the absence of synthesis would produce bursting of the cell. Therefore, within permissible limits, the net balance between wall synthesis and wall lysis will exert a marked influence on the cell growth and morphology. In this work, the b-D-glucosidase activity was firstly selected as a marker of the wall lytic potential of both Rhodotorula glutinis and Rhodotorula mucilaginosa using p-nitrophenyl-β-D-glucopyranoside as substrate. Secondly, a doses-response study involving several metal ions was conducted; as a result, the FeCl3 and MnCl2 significant increased the biomass-bound β-D-glucosidase activity from R. glutinis and R. mucilaginosa, respectively. Thirdly, to test the influence of each metal ion, 6 mM of either FeCl3 (for R. mucilaginosa) or MnCl2 (for R. glutinis) was supplemented to a medium with lower nitrogen content (MLN). The MLN without any addition was taken as the control. Cultures were incubated at 30°C and 180 rpm during six days. Thus, the major and minor diameter of R. mucilaginosa increased from 3.40 ± 0.23 μm to 5.28 ± 0.40 μm and from 2.60 ± 0.23 μm to 5.20 ± 0.55 μm, respectively, in the presence of 6 mM FeCl3. And, the major and minor diameter of R. glutinis increased from 4,90 ± 0.47 μm to 5.48 ± 0.42 μm and from 2.23 ± 0.53 μm to 4.95 ± 0.49 μm, respectively, in the presence of 6 mM MnCl2. So, from these results it might be concluded that the supplementation of either FeCl3 or MnCl2 to the culture medium induced morphological changes in both oleaginous yeasts. In addition, the biomass and lipid concentration obtained after fermentations were also discussed.
Fil: Alfaro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Reyes, Sarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Martearena, Maria Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Blanco, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XII Congreso Argentino de Microbiología General
San Miguel de Tucumán
Argentina
Asociación Civil de Microbiología General
description The cell wall is a complex and dynamic structure. It is also a site of diverse enzyme activities. The balance between its synthesis and lysis is critical, since wall synthesis in the absence of lysis can cause excessive wall thickening and possible growth arrest; while, lysis in the absence of synthesis would produce bursting of the cell. Therefore, within permissible limits, the net balance between wall synthesis and wall lysis will exert a marked influence on the cell growth and morphology. In this work, the b-D-glucosidase activity was firstly selected as a marker of the wall lytic potential of both Rhodotorula glutinis and Rhodotorula mucilaginosa using p-nitrophenyl-β-D-glucopyranoside as substrate. Secondly, a doses-response study involving several metal ions was conducted; as a result, the FeCl3 and MnCl2 significant increased the biomass-bound β-D-glucosidase activity from R. glutinis and R. mucilaginosa, respectively. Thirdly, to test the influence of each metal ion, 6 mM of either FeCl3 (for R. mucilaginosa) or MnCl2 (for R. glutinis) was supplemented to a medium with lower nitrogen content (MLN). The MLN without any addition was taken as the control. Cultures were incubated at 30°C and 180 rpm during six days. Thus, the major and minor diameter of R. mucilaginosa increased from 3.40 ± 0.23 μm to 5.28 ± 0.40 μm and from 2.60 ± 0.23 μm to 5.20 ± 0.55 μm, respectively, in the presence of 6 mM FeCl3. And, the major and minor diameter of R. glutinis increased from 4,90 ± 0.47 μm to 5.48 ± 0.42 μm and from 2.23 ± 0.53 μm to 4.95 ± 0.49 μm, respectively, in the presence of 6 mM MnCl2. So, from these results it might be concluded that the supplementation of either FeCl3 or MnCl2 to the culture medium induced morphological changes in both oleaginous yeasts. In addition, the biomass and lipid concentration obtained after fermentations were also discussed.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188205
Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa; XII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2017; 1-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188205
identifier_str_mv Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa; XII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2017; 1-4
CONICET Digital
CONICET
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language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Asociación Civil de Microbiología General
publisher.none.fl_str_mv Asociación Civil de Microbiología General
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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