Role of simvastatin and methyl-β-cyclodextin on inhibition of poliovirus infection

• Autores: Liu, Shumei; Rodriguez, Ana Virginia; Tosteson, Magdalena T.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Cholesterol; Cyclodextrin; Poliovirus Infection; Simvastatin; Socc
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Cells exposed to simvastatin or to methyl-β-cyclodextrin show reduced poliovirus infection, without alteration in virus binding or on the kinetics of genome entry, suggesting that the steps which are altered are those post uncoating and genome entry. Reduction of infection by cyclodextrin is reversed by increasing MOI whereas that produced by simvastatin treatment is not, suggesting that the effects on infection are not due to a reduction in cholesterol. The differences in the characteristics of inhibition can be explained by the differential effects of the compounds. Cyclodextrin inhibits the store-operated calcium channels, suggesting that reduction in infection is through translational inhibition. Simvastatin produces vesicles from internal membranes which cannot sustain viral RNA synthesis, reducing infection through reduced transcription. The results indicate that the impact on viral infection by the cholesterol-modifying agents is due to the cellular changes produced rather than due to disruption of the cholesterol-rich domains.
Fil: Liu, Shumei. Harvard Medical School; Estados Unidos
Fil: Rodriguez, Ana Virginia. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tosteson, Magdalena T.. Harvard Medical School; Estados Unidos