Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains

Autores
Rochat, Tatiana; Gratadoux, Jean Jacques; Cortier, Gerard; Coqueran, Berard; Nader, Maria Elena Fatima; Gruss, Alexandra; Langella, Philippe
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Numerous industrial bacteria generate hydrogen peroxide (H 2O2), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H2O2. Three SpOx mutants displayed greater H2O2 resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H2O2-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.
Fil: Rochat, Tatiana. Institut National de la Recherche Agronomique; Francia
Fil: Gratadoux, Jean Jacques. Institut National de la Recherche Agronomique; Francia
Fil: Cortier, Gerard. Institut National de la Recherche Agronomique; Francia
Fil: Coqueran, Berard. Institut National de la Recherche Agronomique; Francia
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gruss, Alexandra. Institut National de la Recherche Agronomique; Francia
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
Materia
Lactobacilli
Hydrogen Peroxide
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57289
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/57289
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strainsRochat, TatianaGratadoux, Jean JacquesCortier, GerardCoqueran, BerardNader, Maria Elena FatimaGruss, AlexandraLangella, PhilippeLactobacilliHydrogen PeroxideOxidative Stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Numerous industrial bacteria generate hydrogen peroxide (H 2O2), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H2O2. Three SpOx mutants displayed greater H2O2 resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H2O2-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.Fil: Rochat, Tatiana. Institut National de la Recherche Agronomique; FranciaFil: Gratadoux, Jean Jacques. Institut National de la Recherche Agronomique; FranciaFil: Cortier, Gerard. Institut National de la Recherche Agronomique; FranciaFil: Coqueran, Berard. Institut National de la Recherche Agronomique; FranciaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gruss, Alexandra. Institut National de la Recherche Agronomique; FranciaFil: Langella, Philippe. Institut National de la Recherche Agronomique; FranciaAmerican Society for Microbiology2005-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57289Rochat, Tatiana; Gratadoux, Jean Jacques; Cortier, Gerard; Coqueran, Berard; Nader, Maria Elena Fatima; et al.; Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains; American Society for Microbiology; Applied And Environmental Microbiology; 71; 5; 5-2005; 2782-27880099-22401098-5336CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.71.5.2782-2788.2005info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/71/5/2782info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:14Zoai:ri.conicet.gov.ar:11336/57289instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:14.623CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
title Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
spellingShingle Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
Rochat, Tatiana
Lactobacilli
Hydrogen Peroxide
Oxidative Stress
title_short Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
title_full Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
title_fullStr Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
title_full_unstemmed Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
title_sort Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains
dc.creator.none.fl_str_mv Rochat, Tatiana
Gratadoux, Jean Jacques
Cortier, Gerard
Coqueran, Berard
Nader, Maria Elena Fatima
Gruss, Alexandra
Langella, Philippe
author Rochat, Tatiana
author_facet Rochat, Tatiana
Gratadoux, Jean Jacques
Cortier, Gerard
Coqueran, Berard
Nader, Maria Elena Fatima
Gruss, Alexandra
Langella, Philippe
author_role author
author2 Gratadoux, Jean Jacques
Cortier, Gerard
Coqueran, Berard
Nader, Maria Elena Fatima
Gruss, Alexandra
Langella, Philippe
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Lactobacilli
Hydrogen Peroxide
Oxidative Stress
topic Lactobacilli
Hydrogen Peroxide
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Numerous industrial bacteria generate hydrogen peroxide (H 2O2), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H2O2. Three SpOx mutants displayed greater H2O2 resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H2O2-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.
Fil: Rochat, Tatiana. Institut National de la Recherche Agronomique; Francia
Fil: Gratadoux, Jean Jacques. Institut National de la Recherche Agronomique; Francia
Fil: Cortier, Gerard. Institut National de la Recherche Agronomique; Francia
Fil: Coqueran, Berard. Institut National de la Recherche Agronomique; Francia
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gruss, Alexandra. Institut National de la Recherche Agronomique; Francia
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
description Numerous industrial bacteria generate hydrogen peroxide (H 2O2), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H2O2. Three SpOx mutants displayed greater H2O2 resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H2O2-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.
publishDate 2005
dc.date.none.fl_str_mv 2005-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57289
Rochat, Tatiana; Gratadoux, Jean Jacques; Cortier, Gerard; Coqueran, Berard; Nader, Maria Elena Fatima; et al.; Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains; American Society for Microbiology; Applied And Environmental Microbiology; 71; 5; 5-2005; 2782-2788
0099-2240
1098-5336
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57289
identifier_str_mv Rochat, Tatiana; Gratadoux, Jean Jacques; Cortier, Gerard; Coqueran, Berard; Nader, Maria Elena Fatima; et al.; Lactococcus lactis SpOx spontaneous mutants: A family of oxidative-stress-resistant dairy strains; American Society for Microbiology; Applied And Environmental Microbiology; 71; 5; 5-2005; 2782-2788
0099-2240
1098-5336
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.71.5.2782-2788.2005
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/71/5/2782
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613331091980288
score 13.070432

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