Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases

Autores
Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The retentive behavior of weak acids and bases in reversed-phase liquid chromatography (RPLC) upon changes in column temperature has been theoretically and experimentally studied. The study focuses on examining the temperature dependence of the retention of various solutes at eluent pH close to their corresponding pKa values, and on the indirect role exerted by the buffer ionization equilibria on retention and selectivity. Retention factors of several ionizable compounds in a typical octadecylsilica column and using buffer solutions dissolved in 30% (v/v) acetonitrile as eluent at five temperatures in the range from 25 to 50 °C were carefully measured. Six buffer solutions were prepared from judiciously chosen conjugated pairs of different chemical nature. Their pKa values in this acetonitrile–water composition and within the range of 15–50 °C were determined potentiometrically. These compounds exhibit very different standard ionization enthalpies within this temperature range. Thus, whenever they are used to control mobile phase pH, the column temperature determines their final pH. Predictive equations of retention that take into account the temperature effect on both the transfer and the ionization processes are evaluated. This study demonstrates the significant role that the selected buffer would have on retention and selectivity in RPLC at temperatures higher than 25 °C, particularly for solutes that coelute.
Fil: Gagliardi, Leonardo Gabriel. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ràfols, Clara. Universidad de Barcelona; España
Fil: Rosés, Martí. Universidad de Barcelona; España
Fil: Bosch, Elisabeth. Universidad de Barcelona; España
Materia
ACETONITRILE-WATER MIXTURES
ACID-BASE EQUILIBRIA
IONIZABLE SOLUTES
REVERSED-PHASE SELECTIVITY
TEMPERATURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95453
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95453
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phasesGagliardi, Leonardo GabrielCastells, Cecilia Beatriz MartaRàfols, ClaraRosés, MartíBosch, ElisabethACETONITRILE-WATER MIXTURESACID-BASE EQUILIBRIAIONIZABLE SOLUTESREVERSED-PHASE SELECTIVITYTEMPERATUREhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The retentive behavior of weak acids and bases in reversed-phase liquid chromatography (RPLC) upon changes in column temperature has been theoretically and experimentally studied. The study focuses on examining the temperature dependence of the retention of various solutes at eluent pH close to their corresponding pKa values, and on the indirect role exerted by the buffer ionization equilibria on retention and selectivity. Retention factors of several ionizable compounds in a typical octadecylsilica column and using buffer solutions dissolved in 30% (v/v) acetonitrile as eluent at five temperatures in the range from 25 to 50 °C were carefully measured. Six buffer solutions were prepared from judiciously chosen conjugated pairs of different chemical nature. Their pKa values in this acetonitrile–water composition and within the range of 15–50 °C were determined potentiometrically. These compounds exhibit very different standard ionization enthalpies within this temperature range. Thus, whenever they are used to control mobile phase pH, the column temperature determines their final pH. Predictive equations of retention that take into account the temperature effect on both the transfer and the ionization processes are evaluated. This study demonstrates the significant role that the selected buffer would have on retention and selectivity in RPLC at temperatures higher than 25 °C, particularly for solutes that coelute.Fil: Gagliardi, Leonardo Gabriel. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ràfols, Clara. Universidad de Barcelona; EspañaFil: Rosés, Martí. Universidad de Barcelona; EspañaFil: Bosch, Elisabeth. Universidad de Barcelona; EspañaElsevier Science2005-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95453Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases; Elsevier Science; Journal of Chromatography - A; 1077; 2; 6-2005; 159-1690021-9673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021967305008605info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2005.04.070info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:44Zoai:ri.conicet.gov.ar:11336/95453instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:44.803CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
title Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
spellingShingle Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
Gagliardi, Leonardo Gabriel
ACETONITRILE-WATER MIXTURES
ACID-BASE EQUILIBRIA
IONIZABLE SOLUTES
REVERSED-PHASE SELECTIVITY
TEMPERATURE
title_short Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
title_full Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
title_fullStr Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
title_full_unstemmed Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
title_sort Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases
dc.creator.none.fl_str_mv Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author Gagliardi, Leonardo Gabriel
author_facet Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author_role author
author2 Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACETONITRILE-WATER MIXTURES
ACID-BASE EQUILIBRIA
IONIZABLE SOLUTES
REVERSED-PHASE SELECTIVITY
TEMPERATURE
topic ACETONITRILE-WATER MIXTURES
ACID-BASE EQUILIBRIA
IONIZABLE SOLUTES
REVERSED-PHASE SELECTIVITY
TEMPERATURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The retentive behavior of weak acids and bases in reversed-phase liquid chromatography (RPLC) upon changes in column temperature has been theoretically and experimentally studied. The study focuses on examining the temperature dependence of the retention of various solutes at eluent pH close to their corresponding pKa values, and on the indirect role exerted by the buffer ionization equilibria on retention and selectivity. Retention factors of several ionizable compounds in a typical octadecylsilica column and using buffer solutions dissolved in 30% (v/v) acetonitrile as eluent at five temperatures in the range from 25 to 50 °C were carefully measured. Six buffer solutions were prepared from judiciously chosen conjugated pairs of different chemical nature. Their pKa values in this acetonitrile–water composition and within the range of 15–50 °C were determined potentiometrically. These compounds exhibit very different standard ionization enthalpies within this temperature range. Thus, whenever they are used to control mobile phase pH, the column temperature determines their final pH. Predictive equations of retention that take into account the temperature effect on both the transfer and the ionization processes are evaluated. This study demonstrates the significant role that the selected buffer would have on retention and selectivity in RPLC at temperatures higher than 25 °C, particularly for solutes that coelute.
Fil: Gagliardi, Leonardo Gabriel. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ràfols, Clara. Universidad de Barcelona; España
Fil: Rosés, Martí. Universidad de Barcelona; España
Fil: Bosch, Elisabeth. Universidad de Barcelona; España
description The retentive behavior of weak acids and bases in reversed-phase liquid chromatography (RPLC) upon changes in column temperature has been theoretically and experimentally studied. The study focuses on examining the temperature dependence of the retention of various solutes at eluent pH close to their corresponding pKa values, and on the indirect role exerted by the buffer ionization equilibria on retention and selectivity. Retention factors of several ionizable compounds in a typical octadecylsilica column and using buffer solutions dissolved in 30% (v/v) acetonitrile as eluent at five temperatures in the range from 25 to 50 °C were carefully measured. Six buffer solutions were prepared from judiciously chosen conjugated pairs of different chemical nature. Their pKa values in this acetonitrile–water composition and within the range of 15–50 °C were determined potentiometrically. These compounds exhibit very different standard ionization enthalpies within this temperature range. Thus, whenever they are used to control mobile phase pH, the column temperature determines their final pH. Predictive equations of retention that take into account the temperature effect on both the transfer and the ionization processes are evaluated. This study demonstrates the significant role that the selected buffer would have on retention and selectivity in RPLC at temperatures higher than 25 °C, particularly for solutes that coelute.
publishDate 2005
dc.date.none.fl_str_mv 2005-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95453
Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases; Elsevier Science; Journal of Chromatography - A; 1077; 2; 6-2005; 159-169
0021-9673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95453
identifier_str_mv Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; Effect of temperature on the chromatographic retention of ionizable compounds: II. Acetonitrile–water mobile phases; Elsevier Science; Journal of Chromatography - A; 1077; 2; 6-2005; 159-169
0021-9673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021967305008605
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2005.04.070
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613115523629056
score 13.070432

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