Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity

Autores
Suñol, Cristina; Babot, Zoila; Fonfría, Elena; Galofré, Mireia; Garcia, Daniel Asmed; Herrera, Nancy; Iraola, Susana; Vendrell, Iolanda
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neurotoxicology considers that chemicals perturb neurological functions by interfering with the structure or function of neural pathways, circuits and systems. Using in vitro methods for neurotoxicity studies should include evaluation of specific targets for the functionalism of the nervous system and general cellular targets. In this review we present the neuronal characteristics of primary cultures of cortical neurons and of cerebellar granule cells and their use in neurotoxicity studies. Primary cultures of cortical neurons are constituted by around 40% of GABAergic neurons, whereas primary cultures of cerebellar granule cells are mainly constituted by glutamatergic neurons. Both cultures express functional GABAA and ionotropic glutamate receptors. We present neurotoxicity studies performed in these cell cultures, where specific neural targets related to GABA and glutamate neurotransmission are evaluated. The effects of convulsant polychlorocycloalkane pesticides on the GABAA, glycine and NMDA receptors points to the GABAA receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance might be relevant for long-term toxicity. Several compounds from a list of reference compounds, whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins. We conclude that the in vitro assays that have been developed can be useful for their inclusion in an in vitro test battery to predict human toxicity.
Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
Fil: Babot, Zoila. Consejo Superior de Investigaciones Científicas; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Fonfría, Elena. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Galofré, Mireia. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentina. Consejo Superior de Investigaciones Científicas; España
Fil: Herrera, Nancy. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Iraola, Susana. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Vendrell, Iolanda. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
Materia
Gaba
Glutamate
In Vitro
Neurotoxicity
Primary Neuronal Cultures
Proteomics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57742
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/57742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicitySuñol, CristinaBabot, ZoilaFonfría, ElenaGalofré, MireiaGarcia, Daniel AsmedHerrera, NancyIraola, SusanaVendrell, IolandaGabaGlutamateIn VitroNeurotoxicityPrimary Neuronal CulturesProteomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neurotoxicology considers that chemicals perturb neurological functions by interfering with the structure or function of neural pathways, circuits and systems. Using in vitro methods for neurotoxicity studies should include evaluation of specific targets for the functionalism of the nervous system and general cellular targets. In this review we present the neuronal characteristics of primary cultures of cortical neurons and of cerebellar granule cells and their use in neurotoxicity studies. Primary cultures of cortical neurons are constituted by around 40% of GABAergic neurons, whereas primary cultures of cerebellar granule cells are mainly constituted by glutamatergic neurons. Both cultures express functional GABAA and ionotropic glutamate receptors. We present neurotoxicity studies performed in these cell cultures, where specific neural targets related to GABA and glutamate neurotransmission are evaluated. The effects of convulsant polychlorocycloalkane pesticides on the GABAA, glycine and NMDA receptors points to the GABAA receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance might be relevant for long-term toxicity. Several compounds from a list of reference compounds, whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins. We conclude that the in vitro assays that have been developed can be useful for their inclusion in an in vitro test battery to predict human toxicity.Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; EspañaFil: Babot, Zoila. Consejo Superior de Investigaciones Científicas; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España. Institut d’Investigacions Biomèdiques de Barcelona; EspañaFil: Fonfría, Elena. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; EspañaFil: Galofré, Mireia. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; EspañaFil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentina. Consejo Superior de Investigaciones Científicas; EspañaFil: Herrera, Nancy. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; EspañaFil: Iraola, Susana. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; EspañaFil: Vendrell, Iolanda. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; EspañaPergamon-Elsevier Science Ltd2008-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57742Suñol, Cristina; Babot, Zoila; Fonfría, Elena; Galofré, Mireia; Garcia, Daniel Asmed; et al.; Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 22; 5; 8-2008; 1350-13550887-2333CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233308000830info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2008.03.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:03Zoai:ri.conicet.gov.ar:11336/57742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:03.993CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
title Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
spellingShingle Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
Suñol, Cristina
Gaba
Glutamate
In Vitro
Neurotoxicity
Primary Neuronal Cultures
Proteomics
title_short Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
title_full Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
title_fullStr Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
title_full_unstemmed Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
title_sort Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity
dc.creator.none.fl_str_mv Suñol, Cristina
Babot, Zoila
Fonfría, Elena
Galofré, Mireia
Garcia, Daniel Asmed
Herrera, Nancy
Iraola, Susana
Vendrell, Iolanda
author Suñol, Cristina
author_facet Suñol, Cristina
Babot, Zoila
Fonfría, Elena
Galofré, Mireia
Garcia, Daniel Asmed
Herrera, Nancy
Iraola, Susana
Vendrell, Iolanda
author_role author
author2 Babot, Zoila
Fonfría, Elena
Galofré, Mireia
Garcia, Daniel Asmed
Herrera, Nancy
Iraola, Susana
Vendrell, Iolanda
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gaba
Glutamate
In Vitro
Neurotoxicity
Primary Neuronal Cultures
Proteomics
topic Gaba
Glutamate
In Vitro
Neurotoxicity
Primary Neuronal Cultures
Proteomics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neurotoxicology considers that chemicals perturb neurological functions by interfering with the structure or function of neural pathways, circuits and systems. Using in vitro methods for neurotoxicity studies should include evaluation of specific targets for the functionalism of the nervous system and general cellular targets. In this review we present the neuronal characteristics of primary cultures of cortical neurons and of cerebellar granule cells and their use in neurotoxicity studies. Primary cultures of cortical neurons are constituted by around 40% of GABAergic neurons, whereas primary cultures of cerebellar granule cells are mainly constituted by glutamatergic neurons. Both cultures express functional GABAA and ionotropic glutamate receptors. We present neurotoxicity studies performed in these cell cultures, where specific neural targets related to GABA and glutamate neurotransmission are evaluated. The effects of convulsant polychlorocycloalkane pesticides on the GABAA, glycine and NMDA receptors points to the GABAA receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance might be relevant for long-term toxicity. Several compounds from a list of reference compounds, whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins. We conclude that the in vitro assays that have been developed can be useful for their inclusion in an in vitro test battery to predict human toxicity.
Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
Fil: Babot, Zoila. Consejo Superior de Investigaciones Científicas; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Fonfría, Elena. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Galofré, Mireia. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentina. Consejo Superior de Investigaciones Científicas; España
Fil: Herrera, Nancy. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Iraola, Susana. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Fil: Vendrell, Iolanda. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España. Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública; España
description Neurotoxicology considers that chemicals perturb neurological functions by interfering with the structure or function of neural pathways, circuits and systems. Using in vitro methods for neurotoxicity studies should include evaluation of specific targets for the functionalism of the nervous system and general cellular targets. In this review we present the neuronal characteristics of primary cultures of cortical neurons and of cerebellar granule cells and their use in neurotoxicity studies. Primary cultures of cortical neurons are constituted by around 40% of GABAergic neurons, whereas primary cultures of cerebellar granule cells are mainly constituted by glutamatergic neurons. Both cultures express functional GABAA and ionotropic glutamate receptors. We present neurotoxicity studies performed in these cell cultures, where specific neural targets related to GABA and glutamate neurotransmission are evaluated. The effects of convulsant polychlorocycloalkane pesticides on the GABAA, glycine and NMDA receptors points to the GABAA receptor as the neural target that accounts for their in vivo acute toxicity, whereas NMDA disturbance might be relevant for long-term toxicity. Several compounds from a list of reference compounds, whose severe human poisoning result in convulsions, inhibited the GABAA receptor. We also present cell proteomic studies showing that the neurotoxic contaminant methylmercury affect mitochondrial proteins. We conclude that the in vitro assays that have been developed can be useful for their inclusion in an in vitro test battery to predict human toxicity.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57742
Suñol, Cristina; Babot, Zoila; Fonfría, Elena; Galofré, Mireia; Garcia, Daniel Asmed; et al.; Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 22; 5; 8-2008; 1350-1355
0887-2333
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57742
identifier_str_mv Suñol, Cristina; Babot, Zoila; Fonfría, Elena; Galofré, Mireia; Garcia, Daniel Asmed; et al.; Studies with neuronal cells: From basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 22; 5; 8-2008; 1350-1355
0887-2333
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233308000830
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2008.03.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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