Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity

Autores
Bianucci, Eliana Carolina; Furlan, Ana Laura; Tordable, Maria del Carmen; Hernández, Luis E.; Carpena Ruiz, Ramón O.; Castro, Stella Maris
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Arsenic (As)-polluted groundwater constitutes a serious problem for peanut plants, as roots can accumulate the metalloid in their edible parts. Characterization of stress responses to As may help to detect potential risks and identify mechanisms of tolerance, being the induction of oxidative stress a key feature. Fifteen-day old peanut plants were treated with arsenate in order to characterize the oxidative stress indexes and antioxidant response of the legume under realistic groundwater doses of the metalloid. Superoxide anion (O2[rad]−) and hydrogen peroxide (H2O2) histochemical staining along with the activities of NADPH oxidase, superoxide dismutase (SOD), catalase (CAT) and thiol (glutathione and thioredoxins) metabolism were determined in roots. Results showed that at 20 μM H2AsO4 −, peanut growth was reduced and the root architecture was altered. O2[rad]− and H2O2 accumulated at the root epidermis, while lipid peroxidation, NADPH oxidase, SOD, CAT and glutathione S-transferase (GST) activities augmented. These variables increased with increasing As concentration (100 μM) while glutathione reductase (GR) and glutathione peroxidase/peroxiredoxin (GPX/PRX) were significantly decreased. These findings demonstrated that the metalloid induced physiological and biochemical alterations, being the NADPH oxidase enzyme implicated in the oxidative burst. Additionally, the strong induction of GST activity, even at the lowest H2AsO4 − doses studied, can be exploited as suitable biomarker of As toxicity in peanut plants, which may help to detect risks of As accumulation and select tolerant cultivars.
Fil: Bianucci, Eliana Carolina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Autónoma de Madrid; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Furlan, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tordable, Maria del Carmen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Hernández, Luis E.. Universidad Autónoma de Madrid; España
Fil: Carpena Ruiz, Ramón O.. Universidad Autónoma de Madrid; España
Fil: Castro, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ARSENIC
GLUTATHIONE S-TRANSFERASE
OXIDATIVE STRESS
PEANUT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75346
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicityBianucci, Eliana CarolinaFurlan, Ana LauraTordable, Maria del CarmenHernández, Luis E.Carpena Ruiz, Ramón O.Castro, Stella MarisARSENICGLUTATHIONE S-TRANSFERASEOXIDATIVE STRESSPEANUThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arsenic (As)-polluted groundwater constitutes a serious problem for peanut plants, as roots can accumulate the metalloid in their edible parts. Characterization of stress responses to As may help to detect potential risks and identify mechanisms of tolerance, being the induction of oxidative stress a key feature. Fifteen-day old peanut plants were treated with arsenate in order to characterize the oxidative stress indexes and antioxidant response of the legume under realistic groundwater doses of the metalloid. Superoxide anion (O2[rad]−) and hydrogen peroxide (H2O2) histochemical staining along with the activities of NADPH oxidase, superoxide dismutase (SOD), catalase (CAT) and thiol (glutathione and thioredoxins) metabolism were determined in roots. Results showed that at 20 μM H2AsO4 −, peanut growth was reduced and the root architecture was altered. O2[rad]− and H2O2 accumulated at the root epidermis, while lipid peroxidation, NADPH oxidase, SOD, CAT and glutathione S-transferase (GST) activities augmented. These variables increased with increasing As concentration (100 μM) while glutathione reductase (GR) and glutathione peroxidase/peroxiredoxin (GPX/PRX) were significantly decreased. These findings demonstrated that the metalloid induced physiological and biochemical alterations, being the NADPH oxidase enzyme implicated in the oxidative burst. Additionally, the strong induction of GST activity, even at the lowest H2AsO4 − doses studied, can be exploited as suitable biomarker of As toxicity in peanut plants, which may help to detect risks of As accumulation and select tolerant cultivars.Fil: Bianucci, Eliana Carolina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Autónoma de Madrid; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Furlan, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tordable, Maria del Carmen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Hernández, Luis E.. Universidad Autónoma de Madrid; EspañaFil: Carpena Ruiz, Ramón O.. Universidad Autónoma de Madrid; EspañaFil: Castro, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPergamon-Elsevier Science Ltd2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75346Bianucci, Eliana Carolina; Furlan, Ana Laura; Tordable, Maria del Carmen; Hernández, Luis E.; Carpena Ruiz, Ramón O.; et al.; Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity; Pergamon-Elsevier Science Ltd; Chemosphere; 181; 8-2017; 551-5610045-6535CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2017.04.104info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0045653517306422info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:07Zoai:ri.conicet.gov.ar:11336/75346instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:07.974CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
title Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
spellingShingle Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
Bianucci, Eliana Carolina
ARSENIC
GLUTATHIONE S-TRANSFERASE
OXIDATIVE STRESS
PEANUT
title_short Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
title_full Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
title_fullStr Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
title_full_unstemmed Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
title_sort Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity
dc.creator.none.fl_str_mv Bianucci, Eliana Carolina
Furlan, Ana Laura
Tordable, Maria del Carmen
Hernández, Luis E.
Carpena Ruiz, Ramón O.
Castro, Stella Maris
author Bianucci, Eliana Carolina
author_facet Bianucci, Eliana Carolina
Furlan, Ana Laura
Tordable, Maria del Carmen
Hernández, Luis E.
Carpena Ruiz, Ramón O.
Castro, Stella Maris
author_role author
author2 Furlan, Ana Laura
Tordable, Maria del Carmen
Hernández, Luis E.
Carpena Ruiz, Ramón O.
Castro, Stella Maris
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ARSENIC
GLUTATHIONE S-TRANSFERASE
OXIDATIVE STRESS
PEANUT
topic ARSENIC
GLUTATHIONE S-TRANSFERASE
OXIDATIVE STRESS
PEANUT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Arsenic (As)-polluted groundwater constitutes a serious problem for peanut plants, as roots can accumulate the metalloid in their edible parts. Characterization of stress responses to As may help to detect potential risks and identify mechanisms of tolerance, being the induction of oxidative stress a key feature. Fifteen-day old peanut plants were treated with arsenate in order to characterize the oxidative stress indexes and antioxidant response of the legume under realistic groundwater doses of the metalloid. Superoxide anion (O2[rad]−) and hydrogen peroxide (H2O2) histochemical staining along with the activities of NADPH oxidase, superoxide dismutase (SOD), catalase (CAT) and thiol (glutathione and thioredoxins) metabolism were determined in roots. Results showed that at 20 μM H2AsO4 −, peanut growth was reduced and the root architecture was altered. O2[rad]− and H2O2 accumulated at the root epidermis, while lipid peroxidation, NADPH oxidase, SOD, CAT and glutathione S-transferase (GST) activities augmented. These variables increased with increasing As concentration (100 μM) while glutathione reductase (GR) and glutathione peroxidase/peroxiredoxin (GPX/PRX) were significantly decreased. These findings demonstrated that the metalloid induced physiological and biochemical alterations, being the NADPH oxidase enzyme implicated in the oxidative burst. Additionally, the strong induction of GST activity, even at the lowest H2AsO4 − doses studied, can be exploited as suitable biomarker of As toxicity in peanut plants, which may help to detect risks of As accumulation and select tolerant cultivars.
Fil: Bianucci, Eliana Carolina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Universidad Autónoma de Madrid; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Furlan, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tordable, Maria del Carmen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
Fil: Hernández, Luis E.. Universidad Autónoma de Madrid; España
Fil: Carpena Ruiz, Ramón O.. Universidad Autónoma de Madrid; España
Fil: Castro, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Arsenic (As)-polluted groundwater constitutes a serious problem for peanut plants, as roots can accumulate the metalloid in their edible parts. Characterization of stress responses to As may help to detect potential risks and identify mechanisms of tolerance, being the induction of oxidative stress a key feature. Fifteen-day old peanut plants were treated with arsenate in order to characterize the oxidative stress indexes and antioxidant response of the legume under realistic groundwater doses of the metalloid. Superoxide anion (O2[rad]−) and hydrogen peroxide (H2O2) histochemical staining along with the activities of NADPH oxidase, superoxide dismutase (SOD), catalase (CAT) and thiol (glutathione and thioredoxins) metabolism were determined in roots. Results showed that at 20 μM H2AsO4 −, peanut growth was reduced and the root architecture was altered. O2[rad]− and H2O2 accumulated at the root epidermis, while lipid peroxidation, NADPH oxidase, SOD, CAT and glutathione S-transferase (GST) activities augmented. These variables increased with increasing As concentration (100 μM) while glutathione reductase (GR) and glutathione peroxidase/peroxiredoxin (GPX/PRX) were significantly decreased. These findings demonstrated that the metalloid induced physiological and biochemical alterations, being the NADPH oxidase enzyme implicated in the oxidative burst. Additionally, the strong induction of GST activity, even at the lowest H2AsO4 − doses studied, can be exploited as suitable biomarker of As toxicity in peanut plants, which may help to detect risks of As accumulation and select tolerant cultivars.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75346
Bianucci, Eliana Carolina; Furlan, Ana Laura; Tordable, Maria del Carmen; Hernández, Luis E.; Carpena Ruiz, Ramón O.; et al.; Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity; Pergamon-Elsevier Science Ltd; Chemosphere; 181; 8-2017; 551-561
0045-6535
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75346
identifier_str_mv Bianucci, Eliana Carolina; Furlan, Ana Laura; Tordable, Maria del Carmen; Hernández, Luis E.; Carpena Ruiz, Ramón O.; et al.; Antioxidant responses of peanut roots exposed to realistic groundwater doses of arsenate: Identification of glutathione S-transferase as a suitable biomarker for metalloid toxicity; Pergamon-Elsevier Science Ltd; Chemosphere; 181; 8-2017; 551-561
0045-6535
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2017.04.104
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0045653517306422
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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