Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH

Autores
Cuozzo, Sergio Antonio; Abate, Carlos Mauricio; Amoroso, Maria Julia del R.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Synthesis of dechlorinase in Streptomyces sp. M7 was induced when the microorganism was grown in the presence of lindane (g-HCH) as the only carbon source. Was grown in MM (minimum media) containing 100 mg ml-1 g-HCH and incubated at 30ºC for 48 and 96 h. The inoculum was started with spore suspension (109 c.f.u. ml-1). After incubation pellets were aseptically harvested by centrifugation. The cell-free extract was used for extraction of g-HCH, g-pentachlorocyclohexene (g-PCCH) and 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN).Were extracted by solid phase extraction using C18 columns, evaporated until dry underreduced pressure and the residue was resuspended in hexane. Routine quantitative determinations of lindane catabolism were carried out with gas chromatograph- electron capture detection-mass spectrometry analysis. The g-PCCH and 1,4-TCDN were detected, both being products of the dechlorinase activity from the cell-free extracts obtained at 48 and 96 h of growth of Streptomyces sp. M7 in MM with lindane revealed the appearance of g-PCCH (Rt 6.26 min) and 1,4-TCDN, (Rt 5.29 min), the first and second product of the lindane catabolism by the specific dechlorinase in the catabolic way proposed by Nagata. The relative abundance of g-PCCH and the 1,4- TCDN increased one and half times, at 96 h compared to 48 h of growth, these metabolites were not found in MM with lindane. The first time that the presence of an enzyme with dechlorinase activity has been demonstrated in an actinomycete strain isolated in Tucumán, Argentina.
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
LINDANO
STREPTOMYCETES
BIORREMEDIACION
PLAGUICIDAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194764
Acceder
id CONICETDig_e08f5e13f6092ad2943e4b3898d2b09f
oai_identifier_str oai:ri.conicet.gov.ar:11336/194764
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCHCuozzo, Sergio AntonioAbate, Carlos MauricioAmoroso, Maria Julia del R.LINDANOSTREPTOMYCETESBIORREMEDIACIONPLAGUICIDAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Synthesis of dechlorinase in Streptomyces sp. M7 was induced when the microorganism was grown in the presence of lindane (g-HCH) as the only carbon source. Was grown in MM (minimum media) containing 100 mg ml-1 g-HCH and incubated at 30ºC for 48 and 96 h. The inoculum was started with spore suspension (109 c.f.u. ml-1). After incubation pellets were aseptically harvested by centrifugation. The cell-free extract was used for extraction of g-HCH, g-pentachlorocyclohexene (g-PCCH) and 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN).Were extracted by solid phase extraction using C18 columns, evaporated until dry underreduced pressure and the residue was resuspended in hexane. Routine quantitative determinations of lindane catabolism were carried out with gas chromatograph- electron capture detection-mass spectrometry analysis. The g-PCCH and 1,4-TCDN were detected, both being products of the dechlorinase activity from the cell-free extracts obtained at 48 and 96 h of growth of Streptomyces sp. M7 in MM with lindane revealed the appearance of g-PCCH (Rt 6.26 min) and 1,4-TCDN, (Rt 5.29 min), the first and second product of the lindane catabolism by the specific dechlorinase in the catabolic way proposed by Nagata. The relative abundance of g-PCCH and the 1,4- TCDN increased one and half times, at 96 h compared to 48 h of growth, these metabolites were not found in MM with lindane. The first time that the presence of an enzyme with dechlorinase activity has been demonstrated in an actinomycete strain isolated in Tucumán, Argentina.Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSan Miguel de TucumánArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science Press2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194764Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 108-1080327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v33nSuppl.S/38002Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:30Zoai:ri.conicet.gov.ar:11336/194764instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:31.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
title Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
spellingShingle Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
Cuozzo, Sergio Antonio
LINDANO
STREPTOMYCETES
BIORREMEDIACION
PLAGUICIDAS
title_short Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
title_full Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
title_fullStr Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
title_full_unstemmed Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
title_sort Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH
dc.creator.none.fl_str_mv Cuozzo, Sergio Antonio
Abate, Carlos Mauricio
Amoroso, Maria Julia del R.
author Cuozzo, Sergio Antonio
author_facet Cuozzo, Sergio Antonio
Abate, Carlos Mauricio
Amoroso, Maria Julia del R.
author_role author
author2 Abate, Carlos Mauricio
Amoroso, Maria Julia del R.
author2_role author
author
dc.subject.none.fl_str_mv LINDANO
STREPTOMYCETES
BIORREMEDIACION
PLAGUICIDAS
topic LINDANO
STREPTOMYCETES
BIORREMEDIACION
PLAGUICIDAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Synthesis of dechlorinase in Streptomyces sp. M7 was induced when the microorganism was grown in the presence of lindane (g-HCH) as the only carbon source. Was grown in MM (minimum media) containing 100 mg ml-1 g-HCH and incubated at 30ºC for 48 and 96 h. The inoculum was started with spore suspension (109 c.f.u. ml-1). After incubation pellets were aseptically harvested by centrifugation. The cell-free extract was used for extraction of g-HCH, g-pentachlorocyclohexene (g-PCCH) and 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN).Were extracted by solid phase extraction using C18 columns, evaporated until dry underreduced pressure and the residue was resuspended in hexane. Routine quantitative determinations of lindane catabolism were carried out with gas chromatograph- electron capture detection-mass spectrometry analysis. The g-PCCH and 1,4-TCDN were detected, both being products of the dechlorinase activity from the cell-free extracts obtained at 48 and 96 h of growth of Streptomyces sp. M7 in MM with lindane revealed the appearance of g-PCCH (Rt 6.26 min) and 1,4-TCDN, (Rt 5.29 min), the first and second product of the lindane catabolism by the specific dechlorinase in the catabolic way proposed by Nagata. The relative abundance of g-PCCH and the 1,4- TCDN increased one and half times, at 96 h compared to 48 h of growth, these metabolites were not found in MM with lindane. The first time that the presence of an enzyme with dechlorinase activity has been demonstrated in an actinomycete strain isolated in Tucumán, Argentina.
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
San Miguel de Tucumán
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description The Synthesis of dechlorinase in Streptomyces sp. M7 was induced when the microorganism was grown in the presence of lindane (g-HCH) as the only carbon source. Was grown in MM (minimum media) containing 100 mg ml-1 g-HCH and incubated at 30ºC for 48 and 96 h. The inoculum was started with spore suspension (109 c.f.u. ml-1). After incubation pellets were aseptically harvested by centrifugation. The cell-free extract was used for extraction of g-HCH, g-pentachlorocyclohexene (g-PCCH) and 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN).Were extracted by solid phase extraction using C18 columns, evaporated until dry underreduced pressure and the residue was resuspended in hexane. Routine quantitative determinations of lindane catabolism were carried out with gas chromatograph- electron capture detection-mass spectrometry analysis. The g-PCCH and 1,4-TCDN were detected, both being products of the dechlorinase activity from the cell-free extracts obtained at 48 and 96 h of growth of Streptomyces sp. M7 in MM with lindane revealed the appearance of g-PCCH (Rt 6.26 min) and 1,4-TCDN, (Rt 5.29 min), the first and second product of the lindane catabolism by the specific dechlorinase in the catabolic way proposed by Nagata. The relative abundance of g-PCCH and the 1,4- TCDN increased one and half times, at 96 h compared to 48 h of growth, these metabolites were not found in MM with lindane. The first time that the presence of an enzyme with dechlorinase activity has been demonstrated in an actinomycete strain isolated in Tucumán, Argentina.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194764
Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 108-108
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194764
identifier_str_mv Detection of GAMA-PCCH and 1,4-TCDN in Streptomyces sp. M7cell-free extracts by the dechlorinase activity on degradation GAMA-HCH; XLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; San Miguel de Tucumán; Argentina; 2009; 108-108
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v33nSuppl.S/38002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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