Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats

Autores
Cano, Pilar; Cardinali, Daniel Pedro; Ríos Lugo, María J.; Fernández Mateos, María P.; Reyes Toso, Carlos Felipe; Esquifino, Ana I.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have shown a significant disruption of 24-h pattern of plasma pituitary, adrenal, and gonadal hormones in high-fat-fed rats. Our objective was to assess the effect of a high-fat diet (35% fat) on mean levels and 24-h pattern of several adipocytokines in rats. A normal diet-fed rats (4% fat) were used as controls. When body weight of high-fat-fed rats attained values about 25% higher than controls (after 66 days of treatment), the animals were killed at six different time intervals throughout a 24-h cycle. Plasma concentrations of insulin, adiponectin, interleukin (IL)-1, leptin, ghrelin, plasminogen activator inhibitor-1 (PAI-1), and monocyte chemoattractant protein-1 (MCP-1) were measured in a multianalyte profiling by using the Luminex-100 system. Tumor necrosis factor α (TNFα) and IL-6 were measured by enzyme-linked immunosorbent assay. A significant hyperglycemia developed in high-fat-fed rats, together with a significant increase in plasma insulin. Mean levels of plasma adiponectin, IL-1, IL-6, TNFα, and leptin augmented, and ghrelin decreased, in high-fat-fed rats. The normal daily pattern of plasma insulin, adiponectin, IL-1, IL-6, TNFα, leptin, ghrelin, and MCP-1 became disrupted in high-fat-fed rats. The results indicate that a high-fat diet may bring about signs of insulin resistance and mild inflammation in rats, together with the disruption in daily variations of circulating insulin and ghrelin, and of several adipocytokines including leptin, adiponectin, IL-1, IL-6, TNFα, and MCP-1.
Fil: Cano, Pilar. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Ríos Lugo, María J.. Universidad Complutense de Madrid; España
Fil: Fernández Mateos, María P.. Universidad Complutense de Madrid; España
Fil: Reyes Toso, Carlos Felipe. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Materia
HIGH-FAT-DIET
ADIPOCYTOKINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96247
Acceder
id CONICETDig_e08e98fd488e97b4a89003797e6bfa55
oai_identifier_str oai:ri.conicet.gov.ar:11336/96247
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in ratsCano, PilarCardinali, Daniel PedroRíos Lugo, María J.Fernández Mateos, María P.Reyes Toso, Carlos FelipeEsquifino, Ana I.HIGH-FAT-DIETADIPOCYTOKINEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We have shown a significant disruption of 24-h pattern of plasma pituitary, adrenal, and gonadal hormones in high-fat-fed rats. Our objective was to assess the effect of a high-fat diet (35% fat) on mean levels and 24-h pattern of several adipocytokines in rats. A normal diet-fed rats (4% fat) were used as controls. When body weight of high-fat-fed rats attained values about 25% higher than controls (after 66 days of treatment), the animals were killed at six different time intervals throughout a 24-h cycle. Plasma concentrations of insulin, adiponectin, interleukin (IL)-1, leptin, ghrelin, plasminogen activator inhibitor-1 (PAI-1), and monocyte chemoattractant protein-1 (MCP-1) were measured in a multianalyte profiling by using the Luminex-100 system. Tumor necrosis factor α (TNFα) and IL-6 were measured by enzyme-linked immunosorbent assay. A significant hyperglycemia developed in high-fat-fed rats, together with a significant increase in plasma insulin. Mean levels of plasma adiponectin, IL-1, IL-6, TNFα, and leptin augmented, and ghrelin decreased, in high-fat-fed rats. The normal daily pattern of plasma insulin, adiponectin, IL-1, IL-6, TNFα, leptin, ghrelin, and MCP-1 became disrupted in high-fat-fed rats. The results indicate that a high-fat diet may bring about signs of insulin resistance and mild inflammation in rats, together with the disruption in daily variations of circulating insulin and ghrelin, and of several adipocytokines including leptin, adiponectin, IL-1, IL-6, TNFα, and MCP-1.Fil: Cano, Pilar. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Ríos Lugo, María J.. Universidad Complutense de Madrid; EspañaFil: Fernández Mateos, María P.. Universidad Complutense de Madrid; EspañaFil: Reyes Toso, Carlos Felipe. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaNature Publishing Group2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96247Cano, Pilar; Cardinali, Daniel Pedro; Ríos Lugo, María J.; Fernández Mateos, María P.; Reyes Toso, Carlos Felipe; et al.; Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats; Nature Publishing Group; Obesity; 17; 10; 10-2009; 1866-18711930-7381CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1038/oby.2009.200info:eu-repo/semantics/altIdentifier/doi/10.1038/oby.2009.200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:32Zoai:ri.conicet.gov.ar:11336/96247instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:33.252CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
title Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
spellingShingle Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
Cano, Pilar
HIGH-FAT-DIET
ADIPOCYTOKINES
title_short Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
title_full Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
title_fullStr Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
title_full_unstemmed Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
title_sort Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats
dc.creator.none.fl_str_mv Cano, Pilar
Cardinali, Daniel Pedro
Ríos Lugo, María J.
Fernández Mateos, María P.
Reyes Toso, Carlos Felipe
Esquifino, Ana I.
author Cano, Pilar
author_facet Cano, Pilar
Cardinali, Daniel Pedro
Ríos Lugo, María J.
Fernández Mateos, María P.
Reyes Toso, Carlos Felipe
Esquifino, Ana I.
author_role author
author2 Cardinali, Daniel Pedro
Ríos Lugo, María J.
Fernández Mateos, María P.
Reyes Toso, Carlos Felipe
Esquifino, Ana I.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv HIGH-FAT-DIET
ADIPOCYTOKINES
topic HIGH-FAT-DIET
ADIPOCYTOKINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We have shown a significant disruption of 24-h pattern of plasma pituitary, adrenal, and gonadal hormones in high-fat-fed rats. Our objective was to assess the effect of a high-fat diet (35% fat) on mean levels and 24-h pattern of several adipocytokines in rats. A normal diet-fed rats (4% fat) were used as controls. When body weight of high-fat-fed rats attained values about 25% higher than controls (after 66 days of treatment), the animals were killed at six different time intervals throughout a 24-h cycle. Plasma concentrations of insulin, adiponectin, interleukin (IL)-1, leptin, ghrelin, plasminogen activator inhibitor-1 (PAI-1), and monocyte chemoattractant protein-1 (MCP-1) were measured in a multianalyte profiling by using the Luminex-100 system. Tumor necrosis factor α (TNFα) and IL-6 were measured by enzyme-linked immunosorbent assay. A significant hyperglycemia developed in high-fat-fed rats, together with a significant increase in plasma insulin. Mean levels of plasma adiponectin, IL-1, IL-6, TNFα, and leptin augmented, and ghrelin decreased, in high-fat-fed rats. The normal daily pattern of plasma insulin, adiponectin, IL-1, IL-6, TNFα, leptin, ghrelin, and MCP-1 became disrupted in high-fat-fed rats. The results indicate that a high-fat diet may bring about signs of insulin resistance and mild inflammation in rats, together with the disruption in daily variations of circulating insulin and ghrelin, and of several adipocytokines including leptin, adiponectin, IL-1, IL-6, TNFα, and MCP-1.
Fil: Cano, Pilar. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Ríos Lugo, María J.. Universidad Complutense de Madrid; España
Fil: Fernández Mateos, María P.. Universidad Complutense de Madrid; España
Fil: Reyes Toso, Carlos Felipe. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
description We have shown a significant disruption of 24-h pattern of plasma pituitary, adrenal, and gonadal hormones in high-fat-fed rats. Our objective was to assess the effect of a high-fat diet (35% fat) on mean levels and 24-h pattern of several adipocytokines in rats. A normal diet-fed rats (4% fat) were used as controls. When body weight of high-fat-fed rats attained values about 25% higher than controls (after 66 days of treatment), the animals were killed at six different time intervals throughout a 24-h cycle. Plasma concentrations of insulin, adiponectin, interleukin (IL)-1, leptin, ghrelin, plasminogen activator inhibitor-1 (PAI-1), and monocyte chemoattractant protein-1 (MCP-1) were measured in a multianalyte profiling by using the Luminex-100 system. Tumor necrosis factor α (TNFα) and IL-6 were measured by enzyme-linked immunosorbent assay. A significant hyperglycemia developed in high-fat-fed rats, together with a significant increase in plasma insulin. Mean levels of plasma adiponectin, IL-1, IL-6, TNFα, and leptin augmented, and ghrelin decreased, in high-fat-fed rats. The normal daily pattern of plasma insulin, adiponectin, IL-1, IL-6, TNFα, leptin, ghrelin, and MCP-1 became disrupted in high-fat-fed rats. The results indicate that a high-fat diet may bring about signs of insulin resistance and mild inflammation in rats, together with the disruption in daily variations of circulating insulin and ghrelin, and of several adipocytokines including leptin, adiponectin, IL-1, IL-6, TNFα, and MCP-1.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96247
Cano, Pilar; Cardinali, Daniel Pedro; Ríos Lugo, María J.; Fernández Mateos, María P.; Reyes Toso, Carlos Felipe; et al.; Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats; Nature Publishing Group; Obesity; 17; 10; 10-2009; 1866-1871
1930-7381
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96247
identifier_str_mv Cano, Pilar; Cardinali, Daniel Pedro; Ríos Lugo, María J.; Fernández Mateos, María P.; Reyes Toso, Carlos Felipe; et al.; Effect of a high-fat diet on 24-hour pattern of circulating adipocytokines in rats; Nature Publishing Group; Obesity; 17; 10; 10-2009; 1866-1871
1930-7381
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1038/oby.2009.200
info:eu-repo/semantics/altIdentifier/doi/10.1038/oby.2009.200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269703404257280
score 13.13397

Publicaciones similares