Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean

Autores
Gabbanelli, Valeria; Concha, Francisco Javier; Vazquez, Diego Martin; González Castro, Mariano; Díaz de Astarloa, Juan Martín; Mabragaña, Ezequiel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The yellownose skate, Zearaja chilensis, is a large skate that has been reported from the coasts of Chile in the Southeastern Paci c to Southern Brazilin the Southwestern Atlantic. Due to its geographical range, biological traits, and levels of exploitation, this species was listed as vulnerable by the IUCN.Recent molecular studies have called into question the conspeci city between specimens from these opposite coasts of South America, which can have implications for the conservation status of the species. The aim of this study is to verify the identity of specimens identifed as Z. chilensis in Argentinawith respect to specimens from Chile. A total of 50 specimens from Argentinean waters (AW) and 22 from Chilean waters (CHW) were examined in order to compare their external morphology, spinulation pattern, clasper, and egg capsules. Tissue samples (AW=12, CHW=2) were obtained and sequence data for the Cytochrome Oxidase Subunit I (COI) gene were generated. Additionally, public sequences were obtained from BOLD (AW=25, CHW=8). Despite its external similarity, several raits were consistently different between specimens from both regions. Adults from AW presented longer preorbital length and shorter distance between firstgill openings than those from CHW. AW specimens presented 3-5 rows of caudal horns, whereas most specimens from CHW had only one row. Dermal denticles were restricted to the rostral area in AW skates, whereas in CHW skates most of the dorsal surface of the disc was covered with denticles, with a smooth area in the centre of pectoral fins. Major diferences in clasper morphology were also found.For instance: in AW specimens, terminal bridge (tb) is straight towards tip of dorsal terminal 2 cartilage (dt2) and sentinel (st) does not reach tb, whereasin CHW specimens tb is curved towards tip of dt2 and st reach tb; ventral terminal cartilage of CHW has a longer apophysis and the outer edge is wider than those from AW. Egg capsules of AW skates had wider lateral keels than these of their Chilean counterparts. Molecular analysis revealed two well defined cohesive clusters, corresponding to AW and CHW specimens, respectively. Average K2P distance between groups was 3.4%, a value substantially higher than expected for intraspeci c di erences. Indeed, BINs analysis (algorithm that clusters barcode sequences into OTUs) assigned the sequences to diferent BINs. These integrative results strongly support that specimens from Argentinean waters known as Z. chilensis correspond to a di erent nominal species than those from Chilean waters.
Fil: Gabbanelli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Concha, Francisco Javier. University of Connecticut; Estados Unidos
Fil: Vazquez, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: González Castro, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Díaz de Astarloa, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Mabragaña, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Sharks International Conference
Joao Pessoa
Brasil
Universidade Federal Da Paraiba
Materia
Rajiformes
Longnose Skates
Hidden diversity
Genetics
Morphological features
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267787
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267787
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network_name_str CONICET Digital (CONICET)
spelling Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic OceanGabbanelli, ValeriaConcha, Francisco JavierVazquez, Diego MartinGonzález Castro, MarianoDíaz de Astarloa, Juan MartínMabragaña, EzequielRajiformesLongnose SkatesHidden diversityGeneticsMorphological featureshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The yellownose skate, Zearaja chilensis, is a large skate that has been reported from the coasts of Chile in the Southeastern Paci c to Southern Brazilin the Southwestern Atlantic. Due to its geographical range, biological traits, and levels of exploitation, this species was listed as vulnerable by the IUCN.Recent molecular studies have called into question the conspeci city between specimens from these opposite coasts of South America, which can have implications for the conservation status of the species. The aim of this study is to verify the identity of specimens identifed as Z. chilensis in Argentinawith respect to specimens from Chile. A total of 50 specimens from Argentinean waters (AW) and 22 from Chilean waters (CHW) were examined in order to compare their external morphology, spinulation pattern, clasper, and egg capsules. Tissue samples (AW=12, CHW=2) were obtained and sequence data for the Cytochrome Oxidase Subunit I (COI) gene were generated. Additionally, public sequences were obtained from BOLD (AW=25, CHW=8). Despite its external similarity, several raits were consistently different between specimens from both regions. Adults from AW presented longer preorbital length and shorter distance between firstgill openings than those from CHW. AW specimens presented 3-5 rows of caudal horns, whereas most specimens from CHW had only one row. Dermal denticles were restricted to the rostral area in AW skates, whereas in CHW skates most of the dorsal surface of the disc was covered with denticles, with a smooth area in the centre of pectoral fins. Major diferences in clasper morphology were also found.For instance: in AW specimens, terminal bridge (tb) is straight towards tip of dorsal terminal 2 cartilage (dt2) and sentinel (st) does not reach tb, whereasin CHW specimens tb is curved towards tip of dt2 and st reach tb; ventral terminal cartilage of CHW has a longer apophysis and the outer edge is wider than those from AW. Egg capsules of AW skates had wider lateral keels than these of their Chilean counterparts. Molecular analysis revealed two well defined cohesive clusters, corresponding to AW and CHW specimens, respectively. Average K2P distance between groups was 3.4%, a value substantially higher than expected for intraspeci c di erences. Indeed, BINs analysis (algorithm that clusters barcode sequences into OTUs) assigned the sequences to diferent BINs. These integrative results strongly support that specimens from Argentinean waters known as Z. chilensis correspond to a di erent nominal species than those from Chilean waters.Fil: Gabbanelli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Concha, Francisco Javier. University of Connecticut; Estados UnidosFil: Vazquez, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: González Castro, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Díaz de Astarloa, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Mabragaña, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaSharks International ConferenceJoao PessoaBrasilUniversidade Federal Da ParaibaUniversidade Federal Da Paraiba2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267787Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean; Sharks International Conference; Joao Pessoa; Brasil; 2018; 168-168978-85-237-1396-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sharksinternational.org.br/arquivos/Sharks_International_Conference-Abstract_Book_2.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:23Zoai:ri.conicet.gov.ar:11336/267787instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:24.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
title Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
spellingShingle Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
Gabbanelli, Valeria
Rajiformes
Longnose Skates
Hidden diversity
Genetics
Morphological features
title_short Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
title_full Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
title_fullStr Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
title_full_unstemmed Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
title_sort Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean
dc.creator.none.fl_str_mv Gabbanelli, Valeria
Concha, Francisco Javier
Vazquez, Diego Martin
González Castro, Mariano
Díaz de Astarloa, Juan Martín
Mabragaña, Ezequiel
author Gabbanelli, Valeria
author_facet Gabbanelli, Valeria
Concha, Francisco Javier
Vazquez, Diego Martin
González Castro, Mariano
Díaz de Astarloa, Juan Martín
Mabragaña, Ezequiel
author_role author
author2 Concha, Francisco Javier
Vazquez, Diego Martin
González Castro, Mariano
Díaz de Astarloa, Juan Martín
Mabragaña, Ezequiel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Rajiformes
Longnose Skates
Hidden diversity
Genetics
Morphological features
topic Rajiformes
Longnose Skates
Hidden diversity
Genetics
Morphological features
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The yellownose skate, Zearaja chilensis, is a large skate that has been reported from the coasts of Chile in the Southeastern Paci c to Southern Brazilin the Southwestern Atlantic. Due to its geographical range, biological traits, and levels of exploitation, this species was listed as vulnerable by the IUCN.Recent molecular studies have called into question the conspeci city between specimens from these opposite coasts of South America, which can have implications for the conservation status of the species. The aim of this study is to verify the identity of specimens identifed as Z. chilensis in Argentinawith respect to specimens from Chile. A total of 50 specimens from Argentinean waters (AW) and 22 from Chilean waters (CHW) were examined in order to compare their external morphology, spinulation pattern, clasper, and egg capsules. Tissue samples (AW=12, CHW=2) were obtained and sequence data for the Cytochrome Oxidase Subunit I (COI) gene were generated. Additionally, public sequences were obtained from BOLD (AW=25, CHW=8). Despite its external similarity, several raits were consistently different between specimens from both regions. Adults from AW presented longer preorbital length and shorter distance between firstgill openings than those from CHW. AW specimens presented 3-5 rows of caudal horns, whereas most specimens from CHW had only one row. Dermal denticles were restricted to the rostral area in AW skates, whereas in CHW skates most of the dorsal surface of the disc was covered with denticles, with a smooth area in the centre of pectoral fins. Major diferences in clasper morphology were also found.For instance: in AW specimens, terminal bridge (tb) is straight towards tip of dorsal terminal 2 cartilage (dt2) and sentinel (st) does not reach tb, whereasin CHW specimens tb is curved towards tip of dt2 and st reach tb; ventral terminal cartilage of CHW has a longer apophysis and the outer edge is wider than those from AW. Egg capsules of AW skates had wider lateral keels than these of their Chilean counterparts. Molecular analysis revealed two well defined cohesive clusters, corresponding to AW and CHW specimens, respectively. Average K2P distance between groups was 3.4%, a value substantially higher than expected for intraspeci c di erences. Indeed, BINs analysis (algorithm that clusters barcode sequences into OTUs) assigned the sequences to diferent BINs. These integrative results strongly support that specimens from Argentinean waters known as Z. chilensis correspond to a di erent nominal species than those from Chilean waters.
Fil: Gabbanelli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Concha, Francisco Javier. University of Connecticut; Estados Unidos
Fil: Vazquez, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: González Castro, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Díaz de Astarloa, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Mabragaña, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Sharks International Conference
Joao Pessoa
Brasil
Universidade Federal Da Paraiba
description The yellownose skate, Zearaja chilensis, is a large skate that has been reported from the coasts of Chile in the Southeastern Paci c to Southern Brazilin the Southwestern Atlantic. Due to its geographical range, biological traits, and levels of exploitation, this species was listed as vulnerable by the IUCN.Recent molecular studies have called into question the conspeci city between specimens from these opposite coasts of South America, which can have implications for the conservation status of the species. The aim of this study is to verify the identity of specimens identifed as Z. chilensis in Argentinawith respect to specimens from Chile. A total of 50 specimens from Argentinean waters (AW) and 22 from Chilean waters (CHW) were examined in order to compare their external morphology, spinulation pattern, clasper, and egg capsules. Tissue samples (AW=12, CHW=2) were obtained and sequence data for the Cytochrome Oxidase Subunit I (COI) gene were generated. Additionally, public sequences were obtained from BOLD (AW=25, CHW=8). Despite its external similarity, several raits were consistently different between specimens from both regions. Adults from AW presented longer preorbital length and shorter distance between firstgill openings than those from CHW. AW specimens presented 3-5 rows of caudal horns, whereas most specimens from CHW had only one row. Dermal denticles were restricted to the rostral area in AW skates, whereas in CHW skates most of the dorsal surface of the disc was covered with denticles, with a smooth area in the centre of pectoral fins. Major diferences in clasper morphology were also found.For instance: in AW specimens, terminal bridge (tb) is straight towards tip of dorsal terminal 2 cartilage (dt2) and sentinel (st) does not reach tb, whereasin CHW specimens tb is curved towards tip of dt2 and st reach tb; ventral terminal cartilage of CHW has a longer apophysis and the outer edge is wider than those from AW. Egg capsules of AW skates had wider lateral keels than these of their Chilean counterparts. Molecular analysis revealed two well defined cohesive clusters, corresponding to AW and CHW specimens, respectively. Average K2P distance between groups was 3.4%, a value substantially higher than expected for intraspeci c di erences. Indeed, BINs analysis (algorithm that clusters barcode sequences into OTUs) assigned the sequences to diferent BINs. These integrative results strongly support that specimens from Argentinean waters known as Z. chilensis correspond to a di erent nominal species than those from Chilean waters.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267787
Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean; Sharks International Conference; Joao Pessoa; Brasil; 2018; 168-168
978-85-237-1396-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267787
identifier_str_mv Morphology and DNA Barcoding highlights the presence of a cryptic skate species in the South-west Atlantic Ocean; Sharks International Conference; Joao Pessoa; Brasil; 2018; 168-168
978-85-237-1396-6
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sharksinternational.org.br/arquivos/Sharks_International_Conference-Abstract_Book_2.pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidade Federal Da Paraiba
publisher.none.fl_str_mv Universidade Federal Da Paraiba
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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