Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture

Autores
Bianchi, Mariana; Adur, Javier Fernando; Takizawa, Satoshi; Saida, Kaname; Casco, Victor Hugo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The endothelin system consists of three ligands (ET-1, ET-2 and ET-3) and at least two receptors (ETA and ETB). In mice ET-2 counterpart is a peptide originally called " vasoactive intestinal contractor" (VIC) for this reason, this peptide is frequently named ET-2/VIC. In intestinal villi, fibroblasts-like cells express endothelin's receptors and response to ET-1 and ET-3 peptides, changing their cellular shape. Several functions have been attributed to these peptides in the " architecture" maintenance of intestinal villi acting over sub-epithelial fibroblasts. Despite this, ET-2/VIC has not been analyzed in depth. In this work we show the intestine gene expression and immunolocalization of ET-1, ET-2 and the ETA and ETB receptors from duodenum to rectus and in the villus-crypt axis in mice, allowing a complete analysis of their functions. While ET-1 is expressed uniformly, ET-2 had a particular distribution, being higher at the bottom of the villi of duodenum, ileum and jejunum and reverting this pattern in the crypts of colon and rectus, where the higher expression was at the top. We postulated that ET-2 would act in a cooperative manner with ET-1, giving to the villus the straight enough to withstand mechanical stress.
Fil: Bianchi, Mariana. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Takizawa, Satoshi. Institute for Biological Resources and Functions; Japón
Fil: Saida, Kaname. Soka University; Japón. Institute for Biological Resources and Functions; Japón
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
Materia
ENDOTHELIN
ENDOTHELIN-2
FIBROBLAST NETWORK
INTESTINAL VILLI
SUB-EPITHELIAL FIBROBLASTS
VASOACTIVE INTESTINAL CONTRACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199732
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199732
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architectureBianchi, MarianaAdur, Javier FernandoTakizawa, SatoshiSaida, KanameCasco, Victor HugoENDOTHELINENDOTHELIN-2FIBROBLAST NETWORKINTESTINAL VILLISUB-EPITHELIAL FIBROBLASTSVASOACTIVE INTESTINAL CONTRACTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The endothelin system consists of three ligands (ET-1, ET-2 and ET-3) and at least two receptors (ETA and ETB). In mice ET-2 counterpart is a peptide originally called " vasoactive intestinal contractor" (VIC) for this reason, this peptide is frequently named ET-2/VIC. In intestinal villi, fibroblasts-like cells express endothelin's receptors and response to ET-1 and ET-3 peptides, changing their cellular shape. Several functions have been attributed to these peptides in the " architecture" maintenance of intestinal villi acting over sub-epithelial fibroblasts. Despite this, ET-2/VIC has not been analyzed in depth. In this work we show the intestine gene expression and immunolocalization of ET-1, ET-2 and the ETA and ETB receptors from duodenum to rectus and in the villus-crypt axis in mice, allowing a complete analysis of their functions. While ET-1 is expressed uniformly, ET-2 had a particular distribution, being higher at the bottom of the villi of duodenum, ileum and jejunum and reverting this pattern in the crypts of colon and rectus, where the higher expression was at the top. We postulated that ET-2 would act in a cooperative manner with ET-1, giving to the villus the straight enough to withstand mechanical stress.Fil: Bianchi, Mariana. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Takizawa, Satoshi. Institute for Biological Resources and Functions; JapónFil: Saida, Kaname. Soka University; Japón. Institute for Biological Resources and Functions; JapónFil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; ArgentinaAcademic Press Inc Elsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199732Bianchi, Mariana; Adur, Javier Fernando; Takizawa, Satoshi; Saida, Kaname; Casco, Victor Hugo; Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 417; 4; 1-2012; 1113-11180006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2011.12.053info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:50Zoai:ri.conicet.gov.ar:11336/199732instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:51.193CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
title Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
spellingShingle Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
Bianchi, Mariana
ENDOTHELIN
ENDOTHELIN-2
FIBROBLAST NETWORK
INTESTINAL VILLI
SUB-EPITHELIAL FIBROBLASTS
VASOACTIVE INTESTINAL CONTRACTOR
title_short Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
title_full Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
title_fullStr Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
title_full_unstemmed Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
title_sort Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture
dc.creator.none.fl_str_mv Bianchi, Mariana
Adur, Javier Fernando
Takizawa, Satoshi
Saida, Kaname
Casco, Victor Hugo
author Bianchi, Mariana
author_facet Bianchi, Mariana
Adur, Javier Fernando
Takizawa, Satoshi
Saida, Kaname
Casco, Victor Hugo
author_role author
author2 Adur, Javier Fernando
Takizawa, Satoshi
Saida, Kaname
Casco, Victor Hugo
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENDOTHELIN
ENDOTHELIN-2
FIBROBLAST NETWORK
INTESTINAL VILLI
SUB-EPITHELIAL FIBROBLASTS
VASOACTIVE INTESTINAL CONTRACTOR
topic ENDOTHELIN
ENDOTHELIN-2
FIBROBLAST NETWORK
INTESTINAL VILLI
SUB-EPITHELIAL FIBROBLASTS
VASOACTIVE INTESTINAL CONTRACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The endothelin system consists of three ligands (ET-1, ET-2 and ET-3) and at least two receptors (ETA and ETB). In mice ET-2 counterpart is a peptide originally called " vasoactive intestinal contractor" (VIC) for this reason, this peptide is frequently named ET-2/VIC. In intestinal villi, fibroblasts-like cells express endothelin's receptors and response to ET-1 and ET-3 peptides, changing their cellular shape. Several functions have been attributed to these peptides in the " architecture" maintenance of intestinal villi acting over sub-epithelial fibroblasts. Despite this, ET-2/VIC has not been analyzed in depth. In this work we show the intestine gene expression and immunolocalization of ET-1, ET-2 and the ETA and ETB receptors from duodenum to rectus and in the villus-crypt axis in mice, allowing a complete analysis of their functions. While ET-1 is expressed uniformly, ET-2 had a particular distribution, being higher at the bottom of the villi of duodenum, ileum and jejunum and reverting this pattern in the crypts of colon and rectus, where the higher expression was at the top. We postulated that ET-2 would act in a cooperative manner with ET-1, giving to the villus the straight enough to withstand mechanical stress.
Fil: Bianchi, Mariana. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Takizawa, Satoshi. Institute for Biological Resources and Functions; Japón
Fil: Saida, Kaname. Soka University; Japón. Institute for Biological Resources and Functions; Japón
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopía; Argentina
description The endothelin system consists of three ligands (ET-1, ET-2 and ET-3) and at least two receptors (ETA and ETB). In mice ET-2 counterpart is a peptide originally called " vasoactive intestinal contractor" (VIC) for this reason, this peptide is frequently named ET-2/VIC. In intestinal villi, fibroblasts-like cells express endothelin's receptors and response to ET-1 and ET-3 peptides, changing their cellular shape. Several functions have been attributed to these peptides in the " architecture" maintenance of intestinal villi acting over sub-epithelial fibroblasts. Despite this, ET-2/VIC has not been analyzed in depth. In this work we show the intestine gene expression and immunolocalization of ET-1, ET-2 and the ETA and ETB receptors from duodenum to rectus and in the villus-crypt axis in mice, allowing a complete analysis of their functions. While ET-1 is expressed uniformly, ET-2 had a particular distribution, being higher at the bottom of the villi of duodenum, ileum and jejunum and reverting this pattern in the crypts of colon and rectus, where the higher expression was at the top. We postulated that ET-2 would act in a cooperative manner with ET-1, giving to the villus the straight enough to withstand mechanical stress.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199732
Bianchi, Mariana; Adur, Javier Fernando; Takizawa, Satoshi; Saida, Kaname; Casco, Victor Hugo; Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 417; 4; 1-2012; 1113-1118
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199732
identifier_str_mv Bianchi, Mariana; Adur, Javier Fernando; Takizawa, Satoshi; Saida, Kaname; Casco, Victor Hugo; Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 417; 4; 1-2012; 1113-1118
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2011.12.053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269931254579200
score 13.13397

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