Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease

Autores
Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; Ortega, Hugo Hector
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.
Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina
Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina
Fil: Dupuy, Carlos. Universidad Nacional del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CYSTIC OVARIAN DISEASE
HEAT SHOCK PROTEINS (HSP)
OVARY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97692
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/97692
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heat shock protein patterns in the bovine ovary and relation with cystic ovarian diseaseVelázquez, Melisa María del LujánAlfaro, Natalia SoledadDupuy, CarlosSalvetti, Natalia RaquelRey, FlorenciaOrtega, Hugo HectorCYSTIC OVARIAN DISEASEHEAT SHOCK PROTEINS (HSP)OVARYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; ArgentinaFil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; ArgentinaFil: Dupuy, Carlos. Universidad Nacional del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97692Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-2090378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2009.08.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:56Zoai:ri.conicet.gov.ar:11336/97692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:56.858CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
title Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
spellingShingle Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
Velázquez, Melisa María del Luján
CYSTIC OVARIAN DISEASE
HEAT SHOCK PROTEINS (HSP)
OVARY
title_short Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
title_full Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
title_fullStr Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
title_full_unstemmed Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
title_sort Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
dc.creator.none.fl_str_mv Velázquez, Melisa María del Luján
Alfaro, Natalia Soledad
Dupuy, Carlos
Salvetti, Natalia Raquel
Rey, Florencia
Ortega, Hugo Hector
author Velázquez, Melisa María del Luján
author_facet Velázquez, Melisa María del Luján
Alfaro, Natalia Soledad
Dupuy, Carlos
Salvetti, Natalia Raquel
Rey, Florencia
Ortega, Hugo Hector
author_role author
author2 Alfaro, Natalia Soledad
Dupuy, Carlos
Salvetti, Natalia Raquel
Rey, Florencia
Ortega, Hugo Hector
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CYSTIC OVARIAN DISEASE
HEAT SHOCK PROTEINS (HSP)
OVARY
topic CYSTIC OVARIAN DISEASE
HEAT SHOCK PROTEINS (HSP)
OVARY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.
Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina
Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina
Fil: Dupuy, Carlos. Universidad Nacional del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97692
Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-209
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97692
identifier_str_mv Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-209
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2009.08.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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