Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
- Autores
- Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; Ortega, Hugo Hector
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.
Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina
Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina
Fil: Dupuy, Carlos. Universidad Nacional del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
CYSTIC OVARIAN DISEASE
HEAT SHOCK PROTEINS (HSP)
OVARY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/97692
Ver los metadatos del registro completo
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Heat shock protein patterns in the bovine ovary and relation with cystic ovarian diseaseVelázquez, Melisa María del LujánAlfaro, Natalia SoledadDupuy, CarlosSalvetti, Natalia RaquelRey, FlorenciaOrtega, Hugo HectorCYSTIC OVARIAN DISEASEHEAT SHOCK PROTEINS (HSP)OVARYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; ArgentinaFil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; ArgentinaFil: Dupuy, Carlos. Universidad Nacional del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97692Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-2090378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2009.08.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:56Zoai:ri.conicet.gov.ar:11336/97692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:56.858CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
title |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
spellingShingle |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease Velázquez, Melisa María del Luján CYSTIC OVARIAN DISEASE HEAT SHOCK PROTEINS (HSP) OVARY |
title_short |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
title_full |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
title_fullStr |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
title_full_unstemmed |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
title_sort |
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease |
dc.creator.none.fl_str_mv |
Velázquez, Melisa María del Luján Alfaro, Natalia Soledad Dupuy, Carlos Salvetti, Natalia Raquel Rey, Florencia Ortega, Hugo Hector |
author |
Velázquez, Melisa María del Luján |
author_facet |
Velázquez, Melisa María del Luján Alfaro, Natalia Soledad Dupuy, Carlos Salvetti, Natalia Raquel Rey, Florencia Ortega, Hugo Hector |
author_role |
author |
author2 |
Alfaro, Natalia Soledad Dupuy, Carlos Salvetti, Natalia Raquel Rey, Florencia Ortega, Hugo Hector |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CYSTIC OVARIAN DISEASE HEAT SHOCK PROTEINS (HSP) OVARY |
topic |
CYSTIC OVARIAN DISEASE HEAT SHOCK PROTEINS (HSP) OVARY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis. Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina Fil: Dupuy, Carlos. Universidad Nacional del Litoral; Argentina Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/97692 Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-209 0378-4320 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/97692 |
identifier_str_mv |
Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-209 0378-4320 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2009.08.010 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846082612100071424 |
score |
13.22299 |