Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts

Autores
Salvetti, Natalia Raquel; Baravalle, Celina; Mira, G. A.; Gimeno, Eduardo Juan; Dallard, Bibiana Elisabet; Rey, Florencia; Ortega, Hugo Hector
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic, and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ERá than growing follicles of control individuals. In the theca interna layer, the tertiary follicles presented a significantly higher expression of ERá in the treated group. An increase in the total ERá protein was detected in the treated group. Granulosa cells of all growing, atretic, and cystic follicles show a lower expression of ERâ in animals with COD and the total protein expression of ERâ was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance compared with control animals, and an increase in the HSP70 immunoexpression.
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Mira, G. A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Materia
HEAT SHOCK PROTEIN
CYSTIC OVARIAN DISEASE
BOVINE
STEROID RECEPTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104092
Acceder
id CONICETDig_09a86f57dbfad25a8cdfe4239b01162a
oai_identifier_str oai:ri.conicet.gov.ar:11336/104092
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian CystsSalvetti, Natalia RaquelBaravalle, CelinaMira, G. A.Gimeno, Eduardo JuanDallard, Bibiana ElisabetRey, FlorenciaOrtega, Hugo HectorHEAT SHOCK PROTEINCYSTIC OVARIAN DISEASEBOVINESTEROID RECEPTORhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic, and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ERá than growing follicles of control individuals. In the theca interna layer, the tertiary follicles presented a significantly higher expression of ERá in the treated group. An increase in the total ERá protein was detected in the treated group. Granulosa cells of all growing, atretic, and cystic follicles show a lower expression of ERâ in animals with COD and the total protein expression of ERâ was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance compared with control animals, and an increase in the HSP70 immunoexpression.Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaFil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaFil: Mira, G. A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaFil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaFil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaWiley Blackwell Publishing, Inc2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104092Salvetti, Natalia Raquel; Baravalle, Celina; Mira, G. A.; Gimeno, Eduardo Juan; Dallard, Bibiana Elisabet; et al.; Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 44; 5; 10-2009; 805-8140936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2008.01086.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:08Zoai:ri.conicet.gov.ar:11336/104092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:08.749CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
title Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
spellingShingle Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
Salvetti, Natalia Raquel
HEAT SHOCK PROTEIN
CYSTIC OVARIAN DISEASE
BOVINE
STEROID RECEPTOR
title_short Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
title_full Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
title_fullStr Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
title_full_unstemmed Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
title_sort Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts
dc.creator.none.fl_str_mv Salvetti, Natalia Raquel
Baravalle, Celina
Mira, G. A.
Gimeno, Eduardo Juan
Dallard, Bibiana Elisabet
Rey, Florencia
Ortega, Hugo Hector
author Salvetti, Natalia Raquel
author_facet Salvetti, Natalia Raquel
Baravalle, Celina
Mira, G. A.
Gimeno, Eduardo Juan
Dallard, Bibiana Elisabet
Rey, Florencia
Ortega, Hugo Hector
author_role author
author2 Baravalle, Celina
Mira, G. A.
Gimeno, Eduardo Juan
Dallard, Bibiana Elisabet
Rey, Florencia
Ortega, Hugo Hector
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HEAT SHOCK PROTEIN
CYSTIC OVARIAN DISEASE
BOVINE
STEROID RECEPTOR
topic HEAT SHOCK PROTEIN
CYSTIC OVARIAN DISEASE
BOVINE
STEROID RECEPTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic, and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ERá than growing follicles of control individuals. In the theca interna layer, the tertiary follicles presented a significantly higher expression of ERá in the treated group. An increase in the total ERá protein was detected in the treated group. Granulosa cells of all growing, atretic, and cystic follicles show a lower expression of ERâ in animals with COD and the total protein expression of ERâ was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance compared with control animals, and an increase in the HSP70 immunoexpression.
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Mira, G. A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
description The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic, and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ERá than growing follicles of control individuals. In the theca interna layer, the tertiary follicles presented a significantly higher expression of ERá in the treated group. An increase in the total ERá protein was detected in the treated group. Granulosa cells of all growing, atretic, and cystic follicles show a lower expression of ERâ in animals with COD and the total protein expression of ERâ was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance compared with control animals, and an increase in the HSP70 immunoexpression.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104092
Salvetti, Natalia Raquel; Baravalle, Celina; Mira, G. A.; Gimeno, Eduardo Juan; Dallard, Bibiana Elisabet; et al.; Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 44; 5; 10-2009; 805-814
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104092
identifier_str_mv Salvetti, Natalia Raquel; Baravalle, Celina; Mira, G. A.; Gimeno, Eduardo Juan; Dallard, Bibiana Elisabet; et al.; Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 44; 5; 10-2009; 805-814
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2008.01086.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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