Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism

Autores
Arregger, Alejandro Luis; Contreras, Liliana Noemí; Tumilasci, Omar Rene; Aquilano, Daniel R; Cardoso, Estela M. del Luján
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Summary Objetive The purpose of this study was to demonstrate that Sal-T is a reliable biomarker of androgen status in the diagnosis of male hypogonadism. Design In order to validate the salivary testosterone assay (Sal-T), its reproducibility , the agreement with serum free testosterone levels (Free-T) , the correlation with other circulating androgen markers ( bioavailable testosterone , total testosterone) and  cut off values were defined. Patients and Methods We studied 52 eugonadic (E) and 20 hypogonadic (Hy) men. Sal-T was assayed using an adapted radioimmunoassay for serum testosterone. Sal-T concentrations were compared in 9 cases before and after citric acid stimulation of salivary flow-rate.  Free-T and bioavailable testosterone (Bio-T) were calculated by Vermeulen equation  and SHBG were determined by binding assay. Results Sal-T did not depend on  salivary flow-rate and morning samples from 7.00 to 9.00 h were stable. Agreement between Sal-T and Free-T measurements was confirmed in all subjects .  Sal-T levels correlated positively with  all circulating androgens , showing the best correlation with Free-T in E (r=0.92) as well as in Hy (r=0.97).A cutoff value  for Sal-T  £ 0.195 nM  showed 100% of sensibility and specificity to rule out hypogonadism. Conclusions Our data showed  that Sal-T is a reliable marker of testosterone bioavailability. The results support the inclusion of this biomarker as a non invasive approach in the diagnosis of male androgen deficiency.
Fil: Arregger, Alejandro Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Contreras, Liliana Noemí. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Tumilasci, Omar Rene. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aquilano, Daniel R. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Cardoso, Estela M. del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Materia
salivary testosterone
diagnosis
male hypogonadism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111684
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Salivary testosterone: a reliable approach to the diagnosis of male hypogonadismArregger, Alejandro LuisContreras, Liliana NoemíTumilasci, Omar ReneAquilano, Daniel RCardoso, Estela M. del Lujánsalivary testosteronediagnosismale hypogonadismhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Summary Objetive The purpose of this study was to demonstrate that Sal-T is a reliable biomarker of androgen status in the diagnosis of male hypogonadism. Design In order to validate the salivary testosterone assay (Sal-T), its reproducibility , the agreement with serum free testosterone levels (Free-T) , the correlation with other circulating androgen markers ( bioavailable testosterone , total testosterone) and  cut off values were defined. Patients and Methods We studied 52 eugonadic (E) and 20 hypogonadic (Hy) men. Sal-T was assayed using an adapted radioimmunoassay for serum testosterone. Sal-T concentrations were compared in 9 cases before and after citric acid stimulation of salivary flow-rate.  Free-T and bioavailable testosterone (Bio-T) were calculated by Vermeulen equation  and SHBG were determined by binding assay. Results Sal-T did not depend on  salivary flow-rate and morning samples from 7.00 to 9.00 h were stable. Agreement between Sal-T and Free-T measurements was confirmed in all subjects .  Sal-T levels correlated positively with  all circulating androgens , showing the best correlation with Free-T in E (r=0.92) as well as in Hy (r=0.97).A cutoff value  for Sal-T  £ 0.195 nM  showed 100% of sensibility and specificity to rule out hypogonadism. Conclusions Our data showed  that Sal-T is a reliable marker of testosterone bioavailability. The results support the inclusion of this biomarker as a non invasive approach in the diagnosis of male androgen deficiency.Fil: Arregger, Alejandro Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Contreras, Liliana Noemí. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Tumilasci, Omar Rene. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aquilano, Daniel R. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Cardoso, Estela M. del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaWiley Blackwell Publishing, Inc2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111684Arregger, Alejandro Luis; Contreras, Liliana Noemí; Tumilasci, Omar Rene; Aquilano, Daniel R; Cardoso, Estela M. del Luján; Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism; Wiley Blackwell Publishing, Inc; Clinical Endocrinology; 67; 5; 11-2007; 656-6620300-0664CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2265.2007.02937.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2265.2007.02937.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:14Zoai:ri.conicet.gov.ar:11336/111684instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:14.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
title Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
spellingShingle Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
Arregger, Alejandro Luis
salivary testosterone
diagnosis
male hypogonadism
title_short Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
title_full Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
title_fullStr Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
title_full_unstemmed Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
title_sort Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
dc.creator.none.fl_str_mv Arregger, Alejandro Luis
Contreras, Liliana Noemí
Tumilasci, Omar Rene
Aquilano, Daniel R
Cardoso, Estela M. del Luján
author Arregger, Alejandro Luis
author_facet Arregger, Alejandro Luis
Contreras, Liliana Noemí
Tumilasci, Omar Rene
Aquilano, Daniel R
Cardoso, Estela M. del Luján
author_role author
author2 Contreras, Liliana Noemí
Tumilasci, Omar Rene
Aquilano, Daniel R
Cardoso, Estela M. del Luján
author2_role author
author
author
author
dc.subject.none.fl_str_mv salivary testosterone
diagnosis
male hypogonadism
topic salivary testosterone
diagnosis
male hypogonadism
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Summary Objetive The purpose of this study was to demonstrate that Sal-T is a reliable biomarker of androgen status in the diagnosis of male hypogonadism. Design In order to validate the salivary testosterone assay (Sal-T), its reproducibility , the agreement with serum free testosterone levels (Free-T) , the correlation with other circulating androgen markers ( bioavailable testosterone , total testosterone) and  cut off values were defined. Patients and Methods We studied 52 eugonadic (E) and 20 hypogonadic (Hy) men. Sal-T was assayed using an adapted radioimmunoassay for serum testosterone. Sal-T concentrations were compared in 9 cases before and after citric acid stimulation of salivary flow-rate.  Free-T and bioavailable testosterone (Bio-T) were calculated by Vermeulen equation  and SHBG were determined by binding assay. Results Sal-T did not depend on  salivary flow-rate and morning samples from 7.00 to 9.00 h were stable. Agreement between Sal-T and Free-T measurements was confirmed in all subjects .  Sal-T levels correlated positively with  all circulating androgens , showing the best correlation with Free-T in E (r=0.92) as well as in Hy (r=0.97).A cutoff value  for Sal-T  £ 0.195 nM  showed 100% of sensibility and specificity to rule out hypogonadism. Conclusions Our data showed  that Sal-T is a reliable marker of testosterone bioavailability. The results support the inclusion of this biomarker as a non invasive approach in the diagnosis of male androgen deficiency.
Fil: Arregger, Alejandro Luis. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Contreras, Liliana Noemí. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Tumilasci, Omar Rene. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aquilano, Daniel R. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Cardoso, Estela M. del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
description Summary Objetive The purpose of this study was to demonstrate that Sal-T is a reliable biomarker of androgen status in the diagnosis of male hypogonadism. Design In order to validate the salivary testosterone assay (Sal-T), its reproducibility , the agreement with serum free testosterone levels (Free-T) , the correlation with other circulating androgen markers ( bioavailable testosterone , total testosterone) and  cut off values were defined. Patients and Methods We studied 52 eugonadic (E) and 20 hypogonadic (Hy) men. Sal-T was assayed using an adapted radioimmunoassay for serum testosterone. Sal-T concentrations were compared in 9 cases before and after citric acid stimulation of salivary flow-rate.  Free-T and bioavailable testosterone (Bio-T) were calculated by Vermeulen equation  and SHBG were determined by binding assay. Results Sal-T did not depend on  salivary flow-rate and morning samples from 7.00 to 9.00 h were stable. Agreement between Sal-T and Free-T measurements was confirmed in all subjects .  Sal-T levels correlated positively with  all circulating androgens , showing the best correlation with Free-T in E (r=0.92) as well as in Hy (r=0.97).A cutoff value  for Sal-T  £ 0.195 nM  showed 100% of sensibility and specificity to rule out hypogonadism. Conclusions Our data showed  that Sal-T is a reliable marker of testosterone bioavailability. The results support the inclusion of this biomarker as a non invasive approach in the diagnosis of male androgen deficiency.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111684
Arregger, Alejandro Luis; Contreras, Liliana Noemí; Tumilasci, Omar Rene; Aquilano, Daniel R; Cardoso, Estela M. del Luján; Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism; Wiley Blackwell Publishing, Inc; Clinical Endocrinology; 67; 5; 11-2007; 656-662
0300-0664
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111684
identifier_str_mv Arregger, Alejandro Luis; Contreras, Liliana Noemí; Tumilasci, Omar Rene; Aquilano, Daniel R; Cardoso, Estela M. del Luján; Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism; Wiley Blackwell Publishing, Inc; Clinical Endocrinology; 67; 5; 11-2007; 656-662
0300-0664
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2265.2007.02937.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2265.2007.02937.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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