Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts

Autores
Konrath, Eduardo Luis; Neves, Bruna Medeiros; Lunardi, Paula Santana; Passos, Carolina Dos Santos; Simões Pires, André; Ortega, María Gabriela; Gonçalves, Carlos Alberto; Cabrera, Jose Luis; Fonseca Moreira, José Cláudio; Henriques, Amélia T.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ethnopharmacological relevance The study was aimed at evaluating medicinal and therapeutic potentials of two Lycopodiaceae species, Lycopodium clavatum (L.) and Lycopodium thyoides (Humb. & Bonpl. ex Willd), both used in South American folk medicine for central nervous system conditions. Alkaloid extracts were evaluated for chemical characterization, acetylcholinesterase and antioxidant activities. Materials and methods The alkaloid extracts obtained by alkaline extraction were determined for each species by GC/MS examination. The evaluation of the anticholinesterase and the antioxidant activities of the extracts were tested by determining in vitro and ex vivo models. Effects on acetylcholinesterase (AChE) were tested in vitro using rat brain homogenates and ex vivo after a single administration (25, 10 and 1 mg/kg i.p.) of the alkaloid extracts in mice. The in vitro antioxidant effects were tested for the 2-deoxyribose degradation, nitric oxide (NO) interaction, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and total reactive antioxidant potential (TRAP). After an acute administration (25 and 10 mg/kg i.p.) of the extracts in middle-aged (12 months) mice, the antioxidant effects were estimated through the thiobarbituric acid reactive substances test (TBARS), and the antioxidant enzymes activities for catalase (CAT) and superoxide dismutase (SOD) were measured. Results AChE activity was inhibited in vitro by the alkaloid-enriched extracts of both Lycopodium species in a dose and time-dependent manner in rat cortex, striatum and hippocampus. A significant inhibition was also observed in areas of the brain after acute administration of extracts, as well as decreased lipid peroxidation and increased CAT activity in the cortex, hippocampus and cerebellum. A moderate antioxidant activity was observed in vitro for the extracts. Chemically, the main alkaloids found for the two species were lycopodine and acetyldihidrolycopodine. Conclusion This study showed that the biological properties of the folk medicinal plants Lycopodium clavatum and Lycopodium thyoides include AChE inhibitory activity and antioxidant effects, two possible mechanisms of action in Alzheimer's related processes.
Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Neves, Bruna Medeiros. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Lunardi, Paula Santana. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Passos, Carolina Dos Santos. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Simões Pires, André. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gonçalves, Carlos Alberto. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Fonseca Moreira, José Cláudio. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Henriques, Amélia T.. Universidad Federal do Rio Grande do Sul; Brasil
Materia
Lycopodium Clavatum
Lycopodium Thyoides
Acetylcholinesterase Activity
Antioxidant Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17548
Acceder
id CONICETDig_df302ec6e8d1d09d1340594a14d61a62
oai_identifier_str oai:ri.conicet.gov.ar:11336/17548
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extractsKonrath, Eduardo LuisNeves, Bruna MedeirosLunardi, Paula SantanaPassos, Carolina Dos SantosSimões Pires, AndréOrtega, María GabrielaGonçalves, Carlos AlbertoCabrera, Jose LuisFonseca Moreira, José CláudioHenriques, Amélia T.Lycopodium ClavatumLycopodium ThyoidesAcetylcholinesterase ActivityAntioxidant Activityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Ethnopharmacological relevance The study was aimed at evaluating medicinal and therapeutic potentials of two Lycopodiaceae species, Lycopodium clavatum (L.) and Lycopodium thyoides (Humb. & Bonpl. ex Willd), both used in South American folk medicine for central nervous system conditions. Alkaloid extracts were evaluated for chemical characterization, acetylcholinesterase and antioxidant activities. Materials and methods The alkaloid extracts obtained by alkaline extraction were determined for each species by GC/MS examination. The evaluation of the anticholinesterase and the antioxidant activities of the extracts were tested by determining in vitro and ex vivo models. Effects on acetylcholinesterase (AChE) were tested in vitro using rat brain homogenates and ex vivo after a single administration (25, 10 and 1 mg/kg i.p.) of the alkaloid extracts in mice. The in vitro antioxidant effects were tested for the 2-deoxyribose degradation, nitric oxide (NO) interaction, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and total reactive antioxidant potential (TRAP). After an acute administration (25 and 10 mg/kg i.p.) of the extracts in middle-aged (12 months) mice, the antioxidant effects were estimated through the thiobarbituric acid reactive substances test (TBARS), and the antioxidant enzymes activities for catalase (CAT) and superoxide dismutase (SOD) were measured. Results AChE activity was inhibited in vitro by the alkaloid-enriched extracts of both Lycopodium species in a dose and time-dependent manner in rat cortex, striatum and hippocampus. A significant inhibition was also observed in areas of the brain after acute administration of extracts, as well as decreased lipid peroxidation and increased CAT activity in the cortex, hippocampus and cerebellum. A moderate antioxidant activity was observed in vitro for the extracts. Chemically, the main alkaloids found for the two species were lycopodine and acetyldihidrolycopodine. Conclusion This study showed that the biological properties of the folk medicinal plants Lycopodium clavatum and Lycopodium thyoides include AChE inhibitory activity and antioxidant effects, two possible mechanisms of action in Alzheimer's related processes.Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul; BrasilFil: Neves, Bruna Medeiros. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; BrasilFil: Lunardi, Paula Santana. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; BrasilFil: Passos, Carolina Dos Santos. Universidade Federal do Rio Grande do Sul; BrasilFil: Simões Pires, André. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; BrasilFil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Gonçalves, Carlos Alberto. Universidade Federal do Rio Grande do Sul; BrasilFil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Fonseca Moreira, José Cláudio. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; BrasilFil: Henriques, Amélia T.. Universidad Federal do Rio Grande do Sul; BrasilElsevier Ireland2012-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17548Konrath, Eduardo Luis; Neves, Bruna Medeiros; Lunardi, Paula Santana; Passos, Carolina Dos Santos; Simões Pires, André; et al.; Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts; Elsevier Ireland; Journal Of Ethnopharmacology; 139; 1; 6-1-2012; 58-670378-87411872-7573enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378874111008051info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2011.10.042info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:37Zoai:ri.conicet.gov.ar:11336/17548instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:38.144CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
title Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
spellingShingle Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
Konrath, Eduardo Luis
Lycopodium Clavatum
Lycopodium Thyoides
Acetylcholinesterase Activity
Antioxidant Activity
title_short Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
title_full Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
title_fullStr Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
title_full_unstemmed Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
title_sort Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts
dc.creator.none.fl_str_mv Konrath, Eduardo Luis
Neves, Bruna Medeiros
Lunardi, Paula Santana
Passos, Carolina Dos Santos
Simões Pires, André
Ortega, María Gabriela
Gonçalves, Carlos Alberto
Cabrera, Jose Luis
Fonseca Moreira, José Cláudio
Henriques, Amélia T.
author Konrath, Eduardo Luis
author_facet Konrath, Eduardo Luis
Neves, Bruna Medeiros
Lunardi, Paula Santana
Passos, Carolina Dos Santos
Simões Pires, André
Ortega, María Gabriela
Gonçalves, Carlos Alberto
Cabrera, Jose Luis
Fonseca Moreira, José Cláudio
Henriques, Amélia T.
author_role author
author2 Neves, Bruna Medeiros
Lunardi, Paula Santana
Passos, Carolina Dos Santos
Simões Pires, André
Ortega, María Gabriela
Gonçalves, Carlos Alberto
Cabrera, Jose Luis
Fonseca Moreira, José Cláudio
Henriques, Amélia T.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lycopodium Clavatum
Lycopodium Thyoides
Acetylcholinesterase Activity
Antioxidant Activity
topic Lycopodium Clavatum
Lycopodium Thyoides
Acetylcholinesterase Activity
Antioxidant Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ethnopharmacological relevance The study was aimed at evaluating medicinal and therapeutic potentials of two Lycopodiaceae species, Lycopodium clavatum (L.) and Lycopodium thyoides (Humb. & Bonpl. ex Willd), both used in South American folk medicine for central nervous system conditions. Alkaloid extracts were evaluated for chemical characterization, acetylcholinesterase and antioxidant activities. Materials and methods The alkaloid extracts obtained by alkaline extraction were determined for each species by GC/MS examination. The evaluation of the anticholinesterase and the antioxidant activities of the extracts were tested by determining in vitro and ex vivo models. Effects on acetylcholinesterase (AChE) were tested in vitro using rat brain homogenates and ex vivo after a single administration (25, 10 and 1 mg/kg i.p.) of the alkaloid extracts in mice. The in vitro antioxidant effects were tested for the 2-deoxyribose degradation, nitric oxide (NO) interaction, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and total reactive antioxidant potential (TRAP). After an acute administration (25 and 10 mg/kg i.p.) of the extracts in middle-aged (12 months) mice, the antioxidant effects were estimated through the thiobarbituric acid reactive substances test (TBARS), and the antioxidant enzymes activities for catalase (CAT) and superoxide dismutase (SOD) were measured. Results AChE activity was inhibited in vitro by the alkaloid-enriched extracts of both Lycopodium species in a dose and time-dependent manner in rat cortex, striatum and hippocampus. A significant inhibition was also observed in areas of the brain after acute administration of extracts, as well as decreased lipid peroxidation and increased CAT activity in the cortex, hippocampus and cerebellum. A moderate antioxidant activity was observed in vitro for the extracts. Chemically, the main alkaloids found for the two species were lycopodine and acetyldihidrolycopodine. Conclusion This study showed that the biological properties of the folk medicinal plants Lycopodium clavatum and Lycopodium thyoides include AChE inhibitory activity and antioxidant effects, two possible mechanisms of action in Alzheimer's related processes.
Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Neves, Bruna Medeiros. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Lunardi, Paula Santana. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Passos, Carolina Dos Santos. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Simões Pires, André. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gonçalves, Carlos Alberto. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Fonseca Moreira, José Cláudio. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Henriques, Amélia T.. Universidad Federal do Rio Grande do Sul; Brasil
description Ethnopharmacological relevance The study was aimed at evaluating medicinal and therapeutic potentials of two Lycopodiaceae species, Lycopodium clavatum (L.) and Lycopodium thyoides (Humb. & Bonpl. ex Willd), both used in South American folk medicine for central nervous system conditions. Alkaloid extracts were evaluated for chemical characterization, acetylcholinesterase and antioxidant activities. Materials and methods The alkaloid extracts obtained by alkaline extraction were determined for each species by GC/MS examination. The evaluation of the anticholinesterase and the antioxidant activities of the extracts were tested by determining in vitro and ex vivo models. Effects on acetylcholinesterase (AChE) were tested in vitro using rat brain homogenates and ex vivo after a single administration (25, 10 and 1 mg/kg i.p.) of the alkaloid extracts in mice. The in vitro antioxidant effects were tested for the 2-deoxyribose degradation, nitric oxide (NO) interaction, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and total reactive antioxidant potential (TRAP). After an acute administration (25 and 10 mg/kg i.p.) of the extracts in middle-aged (12 months) mice, the antioxidant effects were estimated through the thiobarbituric acid reactive substances test (TBARS), and the antioxidant enzymes activities for catalase (CAT) and superoxide dismutase (SOD) were measured. Results AChE activity was inhibited in vitro by the alkaloid-enriched extracts of both Lycopodium species in a dose and time-dependent manner in rat cortex, striatum and hippocampus. A significant inhibition was also observed in areas of the brain after acute administration of extracts, as well as decreased lipid peroxidation and increased CAT activity in the cortex, hippocampus and cerebellum. A moderate antioxidant activity was observed in vitro for the extracts. Chemically, the main alkaloids found for the two species were lycopodine and acetyldihidrolycopodine. Conclusion This study showed that the biological properties of the folk medicinal plants Lycopodium clavatum and Lycopodium thyoides include AChE inhibitory activity and antioxidant effects, two possible mechanisms of action in Alzheimer's related processes.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17548
Konrath, Eduardo Luis; Neves, Bruna Medeiros; Lunardi, Paula Santana; Passos, Carolina Dos Santos; Simões Pires, André; et al.; Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts; Elsevier Ireland; Journal Of Ethnopharmacology; 139; 1; 6-1-2012; 58-67
0378-8741
1872-7573
url http://hdl.handle.net/11336/17548
identifier_str_mv Konrath, Eduardo Luis; Neves, Bruna Medeiros; Lunardi, Paula Santana; Passos, Carolina Dos Santos; Simões Pires, André; et al.; Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from south america on alkaloid extracts; Elsevier Ireland; Journal Of Ethnopharmacology; 139; 1; 6-1-2012; 58-67
0378-8741
1872-7573
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378874111008051
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2011.10.042
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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