Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study

Autores
Duvilanski, Beatriz Haydee; Pérez, R.; Seilicovich, Adriana; Lasaga, Mercedes Isabel; Diaz, Maria del Carmen; Debeljuk, L.
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the internalization and intracellular distribution of [3H] GABA in rat anterior pituitary cells. Electron microscopic autoradiography of anterior pituitary fragments or dispersed pituitary cells incubated with[3H] GABA showed that lactotrophs and to a lesser extent somatotrophs were the onlycells that contained radioactive grains. Grain density analysis performed on dispersed pituitarycells after a pulse-chase experiment (10 min pulse and then change to a medium without radioactive GABA for various periods up to 2 h) revealed that GABA internalized by lactotrophs was distributed in various intracellular membranous organelles. Of the cell compartments examined plasma membrane Golgi apparatus mitochondria and secretory granules had different time-dependent labeling patterns. The highest grain density values were associated with plasma membrane (at the first chase time) and the Golgi apparatus. Mitochondria and secretory granules also showed significant grain density values. A similar pattern of distribution was observed when fragments of prolactin-secreting pituitary adenomas were incubatedwith [3H] GABA. These results providemorphological data on the cellular specificity and intracellular distribution of GABA in anterior pituitary cells.
Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, R.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Seilicovich, Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lasaga, Mercedes Isabel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz, Maria del Carmen. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Debeljuk, L.. Southern Illinois University; Estados Unidos
Materia
Anterior Pituitary Cells
Electron Microscopic Autoradiography
Gaba
Pituitary Adenoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40327
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40327
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic studyDuvilanski, Beatriz HaydeePérez, R.Seilicovich, AdrianaLasaga, Mercedes IsabelDiaz, Maria del CarmenDebeljuk, L.Anterior Pituitary CellsElectron Microscopic AutoradiographyGabaPituitary Adenomahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We studied the internalization and intracellular distribution of [3H] GABA in rat anterior pituitary cells. Electron microscopic autoradiography of anterior pituitary fragments or dispersed pituitary cells incubated with[3H] GABA showed that lactotrophs and to a lesser extent somatotrophs were the onlycells that contained radioactive grains. Grain density analysis performed on dispersed pituitarycells after a pulse-chase experiment (10 min pulse and then change to a medium without radioactive GABA for various periods up to 2 h) revealed that GABA internalized by lactotrophs was distributed in various intracellular membranous organelles. Of the cell compartments examined plasma membrane Golgi apparatus mitochondria and secretory granules had different time-dependent labeling patterns. The highest grain density values were associated with plasma membrane (at the first chase time) and the Golgi apparatus. Mitochondria and secretory granules also showed significant grain density values. A similar pattern of distribution was observed when fragments of prolactin-secreting pituitary adenomas were incubatedwith [3H] GABA. These results providemorphological data on the cellular specificity and intracellular distribution of GABA in anterior pituitary cells.Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, R.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Seilicovich, Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lasaga, Mercedes Isabel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diaz, Maria del Carmen. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Debeljuk, L.. Southern Illinois University; Estados UnidosHarcourt Publishers2000-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40327Duvilanski, Beatriz Haydee; Pérez, R.; Seilicovich, Adriana; Lasaga, Mercedes Isabel; Diaz, Maria del Carmen; et al.; Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study; Harcourt Publishers; Tissue & Cell; 32; 4; 4-2000; 284-2920040-8166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1054/tice.2000.0116info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0040816600901162info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:07Zoai:ri.conicet.gov.ar:11336/40327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:07.909CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
title Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
spellingShingle Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
Duvilanski, Beatriz Haydee
Anterior Pituitary Cells
Electron Microscopic Autoradiography
Gaba
Pituitary Adenoma
title_short Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
title_full Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
title_fullStr Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
title_full_unstemmed Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
title_sort Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study
dc.creator.none.fl_str_mv Duvilanski, Beatriz Haydee
Pérez, R.
Seilicovich, Adriana
Lasaga, Mercedes Isabel
Diaz, Maria del Carmen
Debeljuk, L.
author Duvilanski, Beatriz Haydee
author_facet Duvilanski, Beatriz Haydee
Pérez, R.
Seilicovich, Adriana
Lasaga, Mercedes Isabel
Diaz, Maria del Carmen
Debeljuk, L.
author_role author
author2 Pérez, R.
Seilicovich, Adriana
Lasaga, Mercedes Isabel
Diaz, Maria del Carmen
Debeljuk, L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Anterior Pituitary Cells
Electron Microscopic Autoradiography
Gaba
Pituitary Adenoma
topic Anterior Pituitary Cells
Electron Microscopic Autoradiography
Gaba
Pituitary Adenoma
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We studied the internalization and intracellular distribution of [3H] GABA in rat anterior pituitary cells. Electron microscopic autoradiography of anterior pituitary fragments or dispersed pituitary cells incubated with[3H] GABA showed that lactotrophs and to a lesser extent somatotrophs were the onlycells that contained radioactive grains. Grain density analysis performed on dispersed pituitarycells after a pulse-chase experiment (10 min pulse and then change to a medium without radioactive GABA for various periods up to 2 h) revealed that GABA internalized by lactotrophs was distributed in various intracellular membranous organelles. Of the cell compartments examined plasma membrane Golgi apparatus mitochondria and secretory granules had different time-dependent labeling patterns. The highest grain density values were associated with plasma membrane (at the first chase time) and the Golgi apparatus. Mitochondria and secretory granules also showed significant grain density values. A similar pattern of distribution was observed when fragments of prolactin-secreting pituitary adenomas were incubatedwith [3H] GABA. These results providemorphological data on the cellular specificity and intracellular distribution of GABA in anterior pituitary cells.
Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, R.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina
Fil: Seilicovich, Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lasaga, Mercedes Isabel. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diaz, Maria del Carmen. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Debeljuk, L.. Southern Illinois University; Estados Unidos
description We studied the internalization and intracellular distribution of [3H] GABA in rat anterior pituitary cells. Electron microscopic autoradiography of anterior pituitary fragments or dispersed pituitary cells incubated with[3H] GABA showed that lactotrophs and to a lesser extent somatotrophs were the onlycells that contained radioactive grains. Grain density analysis performed on dispersed pituitarycells after a pulse-chase experiment (10 min pulse and then change to a medium without radioactive GABA for various periods up to 2 h) revealed that GABA internalized by lactotrophs was distributed in various intracellular membranous organelles. Of the cell compartments examined plasma membrane Golgi apparatus mitochondria and secretory granules had different time-dependent labeling patterns. The highest grain density values were associated with plasma membrane (at the first chase time) and the Golgi apparatus. Mitochondria and secretory granules also showed significant grain density values. A similar pattern of distribution was observed when fragments of prolactin-secreting pituitary adenomas were incubatedwith [3H] GABA. These results providemorphological data on the cellular specificity and intracellular distribution of GABA in anterior pituitary cells.
publishDate 2000
dc.date.none.fl_str_mv 2000-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40327
Duvilanski, Beatriz Haydee; Pérez, R.; Seilicovich, Adriana; Lasaga, Mercedes Isabel; Diaz, Maria del Carmen; et al.; Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study; Harcourt Publishers; Tissue & Cell; 32; 4; 4-2000; 284-292
0040-8166
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40327
identifier_str_mv Duvilanski, Beatriz Haydee; Pérez, R.; Seilicovich, Adriana; Lasaga, Mercedes Isabel; Diaz, Maria del Carmen; et al.; Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study; Harcourt Publishers; Tissue & Cell; 32; 4; 4-2000; 284-292
0040-8166
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1054/tice.2000.0116
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0040816600901162
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Harcourt Publishers
publisher.none.fl_str_mv Harcourt Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269738779017216
score 13.13397

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