Genetic analysis of an insular population of Sapajus nigritus (Primates: Cebidae) in Rio de Janeiro state, Brazil

• Autores: Mattos Penedo, Diego; Azevedo de Armada, Jorge Luís; Nieves, Mariela; da Silva Verona, Carlos Eduardo; de Oliveira, Andréa Maria; de Sousa dos Santo, Emidio José; Monnerat Nogueira, Denise
• Año de publicación: 2021
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: C-BANDING; CAPUCHIN MONKEYS; CYTOCHROME C OXIDASE I AND II; DNA SEQUENCING; FLUORESCENCE IN SITU HYBRIDIZATION; ILHA DA MARAMBAIA
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The black-horned capuchin (Sapajus nigritus) is a neotropical primate with wide distribution from southeastern Brazil to northeastern Argentina. Although this species has been described with coat pattern variation, even with intrapopulational differences, and characterized as having the greatest genetic diversity among Sapajus species, there are still few studies on natural populations that contribute to the knowledge of this intraspecific variability. We examined individuals from an as yet unstudied population of Ilha da Marambaia, Rio de Janeiro (RJ) state, Brazil, compared with published data for S. nigritus. We sought to confirm the species through phenotypic and genetic characterization using C-banding and fluorescence in situ hybridization with #11qHe+/21WCP probes for chromosomal constitutive heterochromatin (He+) patterns, and cytochrome c oxidase I and II gene sequences for phylogenetic analysis. The coat presented two color patterns, varying from brown to blackish on the body, yellow to brown on the chest, and white to yellow on the face, besides the presence and shape of the tufts on the head, corresponding to S. nigritus. He+ was identified in pairs 4, 12, 13 and 17, and less consistently in pairs 6, 19 and 21, already described for this species. While most Sapajus species have a large He+ block, here pair 11 was identified without extracentromeric He+, the same as reported for S. nigritus from Argentina. Molecular analysis showed divergence of this population from other S. nigritus sequences, reinforcing a trend already demonstrated when samples from RJ are compared with the rest of the distribution, which may represent an evolutionary deviation.
Fil: Mattos Penedo, Diego. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Azevedo de Armada, Jorge Luís. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Nieves, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: da Silva Verona, Carlos Eduardo. No especifíca;
Fil: de Oliveira, Andréa Maria. Universidade Do Estado de Rio Do Janeiro. Instituto de Biologia "roberto Alcantara Gomes".; Brasil
Fil: de Sousa dos Santo, Emidio José. Universidade Federal do Rio de Janeiro; Brasil
Fil: Monnerat Nogueira, Denise. Universidade Federal Rural do Rio de Janeiro; Brasil