Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)

Autores
Gokhman, Vladimir E.; Luna, María Gabriela; Vallina, Consuelo; Bressa, Maria Jose
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several techniques of differential chromosome staining, i.e., C-banding and staining with base-specific fluorochromes, together with fluorescence in situ hybridization (FISH) with an 18S rDNA probe. We found n = 7 and 2n = 14 in P. dignus, with seven metacentric chromosomes of similar size in the haploid set. C-banding revealed various C-positive bands, either centromeric or interstitial, on most chromosomes. Both AT-specific and GC-specific fluorochromes, 4’6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA3) respectively, showed uniform staining of chromosomes. FISH visualized a single subterminal rDNA site on a medium-sized metacentric. A brief review of known chromosome sets of the subfamily Microgastrinae (Braconidae) is given; certain features of karyotype evolution of this group are discussed.
Fil: Gokhman, Vladimir E.. Russian Entomological Society, Moscow; Rusia
Fil: Luna, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universidad Nacional de San Antonio de Areco.; Argentina
Fil: Vallina, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
BASE-SPECIFIC FLUOROCHROMES
BRACONIDAE
C-BANDING
CHROMOSOMES
FLUORESCENCE IN SITU HYBRIDIZATION
KARYOTYPES
MICROGASTRINAE
PARASITOIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/264600
Acceder
id CONICETDig_806e13c2c912fa355f97a18b10aa4687
oai_identifier_str oai:ri.conicet.gov.ar:11336/264600
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)Gokhman, Vladimir E.Luna, María GabrielaVallina, ConsueloBressa, Maria JoseBASE-SPECIFIC FLUOROCHROMESBRACONIDAEC-BANDINGCHROMOSOMESFLUORESCENCE IN SITU HYBRIDIZATIONKARYOTYPESMICROGASTRINAEPARASITOIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several techniques of differential chromosome staining, i.e., C-banding and staining with base-specific fluorochromes, together with fluorescence in situ hybridization (FISH) with an 18S rDNA probe. We found n = 7 and 2n = 14 in P. dignus, with seven metacentric chromosomes of similar size in the haploid set. C-banding revealed various C-positive bands, either centromeric or interstitial, on most chromosomes. Both AT-specific and GC-specific fluorochromes, 4’6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA3) respectively, showed uniform staining of chromosomes. FISH visualized a single subterminal rDNA site on a medium-sized metacentric. A brief review of known chromosome sets of the subfamily Microgastrinae (Braconidae) is given; certain features of karyotype evolution of this group are discussed.Fil: Gokhman, Vladimir E.. Russian Entomological Society, Moscow; RusiaFil: Luna, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universidad Nacional de San Antonio de Areco.; ArgentinaFil: Vallina, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaZoological Institute2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264600Gokhman, Vladimir E.; Luna, María Gabriela; Vallina, Consuelo; Bressa, Maria Jose; Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae); Zoological Institute; Comparative Cytogenetics; 18; 11-2024; 199-2111993-07711993-078XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://compcytogen.pensoft.net/article/133534/info:eu-repo/semantics/altIdentifier/doi/10.3897/compcytogen.18.133534info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:47Zoai:ri.conicet.gov.ar:11336/264600instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:47.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
title Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
spellingShingle Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
Gokhman, Vladimir E.
BASE-SPECIFIC FLUOROCHROMES
BRACONIDAE
C-BANDING
CHROMOSOMES
FLUORESCENCE IN SITU HYBRIDIZATION
KARYOTYPES
MICROGASTRINAE
PARASITOIDS
title_short Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
title_full Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
title_fullStr Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
title_full_unstemmed Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
title_sort Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae)
dc.creator.none.fl_str_mv Gokhman, Vladimir E.
Luna, María Gabriela
Vallina, Consuelo
Bressa, Maria Jose
author Gokhman, Vladimir E.
author_facet Gokhman, Vladimir E.
Luna, María Gabriela
Vallina, Consuelo
Bressa, Maria Jose
author_role author
author2 Luna, María Gabriela
Vallina, Consuelo
Bressa, Maria Jose
author2_role author
author
author
dc.subject.none.fl_str_mv BASE-SPECIFIC FLUOROCHROMES
BRACONIDAE
C-BANDING
CHROMOSOMES
FLUORESCENCE IN SITU HYBRIDIZATION
KARYOTYPES
MICROGASTRINAE
PARASITOIDS
topic BASE-SPECIFIC FLUOROCHROMES
BRACONIDAE
C-BANDING
CHROMOSOMES
FLUORESCENCE IN SITU HYBRIDIZATION
KARYOTYPES
MICROGASTRINAE
PARASITOIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several techniques of differential chromosome staining, i.e., C-banding and staining with base-specific fluorochromes, together with fluorescence in situ hybridization (FISH) with an 18S rDNA probe. We found n = 7 and 2n = 14 in P. dignus, with seven metacentric chromosomes of similar size in the haploid set. C-banding revealed various C-positive bands, either centromeric or interstitial, on most chromosomes. Both AT-specific and GC-specific fluorochromes, 4’6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA3) respectively, showed uniform staining of chromosomes. FISH visualized a single subterminal rDNA site on a medium-sized metacentric. A brief review of known chromosome sets of the subfamily Microgastrinae (Braconidae) is given; certain features of karyotype evolution of this group are discussed.
Fil: Gokhman, Vladimir E.. Russian Entomological Society, Moscow; Rusia
Fil: Luna, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universidad Nacional de San Antonio de Areco.; Argentina
Fil: Vallina, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several techniques of differential chromosome staining, i.e., C-banding and staining with base-specific fluorochromes, together with fluorescence in situ hybridization (FISH) with an 18S rDNA probe. We found n = 7 and 2n = 14 in P. dignus, with seven metacentric chromosomes of similar size in the haploid set. C-banding revealed various C-positive bands, either centromeric or interstitial, on most chromosomes. Both AT-specific and GC-specific fluorochromes, 4’6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA3) respectively, showed uniform staining of chromosomes. FISH visualized a single subterminal rDNA site on a medium-sized metacentric. A brief review of known chromosome sets of the subfamily Microgastrinae (Braconidae) is given; certain features of karyotype evolution of this group are discussed.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/264600
Gokhman, Vladimir E.; Luna, María Gabriela; Vallina, Consuelo; Bressa, Maria Jose; Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae); Zoological Institute; Comparative Cytogenetics; 18; 11-2024; 199-211
1993-0771
1993-078X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/264600
identifier_str_mv Gokhman, Vladimir E.; Luna, María Gabriela; Vallina, Consuelo; Bressa, Maria Jose; Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae); Zoological Institute; Comparative Cytogenetics; 18; 11-2024; 199-211
1993-0771
1993-078X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://compcytogen.pensoft.net/article/133534/
info:eu-repo/semantics/altIdentifier/doi/10.3897/compcytogen.18.133534
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Zoological Institute
publisher.none.fl_str_mv Zoological Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269659050541056
score 13.13397

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