Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques

Autores
López, Alicia; Fernandez, Aveliano; Poggio, Lidia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids.
Fil: López, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Fernandez, Aveliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Poggio, Lidia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina
Materia
GISH-FISH
IN SITU HYBRIDIZATION
TURNERA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99835
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99835
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniquesLópez, AliciaFernandez, AvelianoPoggio, LidiaGISH-FISHIN SITU HYBRIDIZATIONTURNERAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids.Fil: López, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; ArgentinaFil: Fernandez, Aveliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Poggio, Lidia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; ArgentinaNational Research Council Canada-NRC Research Press2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/99835López, Alicia; Fernandez, Aveliano; Poggio, Lidia; Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques; National Research Council Canada-NRC Research Press; Genome; 53; 8; 8-2010; 594-5980831-2796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/G10-037info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/G10-037#.XmKHKnCIqM8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:29Zoai:ri.conicet.gov.ar:11336/99835instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:29.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
title Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
spellingShingle Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
López, Alicia
GISH-FISH
IN SITU HYBRIDIZATION
TURNERA
title_short Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
title_full Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
title_fullStr Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
title_full_unstemmed Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
title_sort Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
dc.creator.none.fl_str_mv López, Alicia
Fernandez, Aveliano
Poggio, Lidia
author López, Alicia
author_facet López, Alicia
Fernandez, Aveliano
Poggio, Lidia
author_role author
author2 Fernandez, Aveliano
Poggio, Lidia
author2_role author
author
dc.subject.none.fl_str_mv GISH-FISH
IN SITU HYBRIDIZATION
TURNERA
topic GISH-FISH
IN SITU HYBRIDIZATION
TURNERA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids.
Fil: López, Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Fernandez, Aveliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Poggio, Lidia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina
description Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99835
López, Alicia; Fernandez, Aveliano; Poggio, Lidia; Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques; National Research Council Canada-NRC Research Press; Genome; 53; 8; 8-2010; 594-598
0831-2796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99835
identifier_str_mv López, Alicia; Fernandez, Aveliano; Poggio, Lidia; Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques; National Research Council Canada-NRC Research Press; Genome; 53; 8; 8-2010; 594-598
0831-2796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/G10-037
info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/G10-037#.XmKHKnCIqM8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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