Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum

Autores
Puighermanal, Emma; Castell, Laia; Esteve Codina, Anna; Melser, Su; Kaganovsky, Konstantin; Zussy, Charleine; Boubaker Vitre, Jihane; Gut, Marta; Rialle, Stephanie; Kellendonk, Christoph; Sanz, Elisenda; Quintana, Albert; Marsicano, Giovanni; Martin, Miquel; Rubinstein, Marcelo; Girault, Jean Antoine; Ding, Jun B.; Valjent, Emmanuel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Action control is a key brain function determining the survival of animals in their environment. In mammals, neurons expressing dopamine D2 receptors (D2R) in the dorsal striatum (DS) and the nucleus accumbens (Acb) jointly but differentially contribute to the fine regulation of movement. However, their region-specific molecular features are presently unknown. By combining RNAseq of striatal D2R neurons and histological analyses, we identified hundreds of novel region-specific molecular markers, which may serve as tools to target selective subpopulations. As a proof of concept, we characterized the molecular identity of a subcircuit defined by WFS1 neurons and evaluated multiple behavioral tasks after its temporally-controlled deletion of D2R. Consequently, conditional D2R knockout mice displayed a significant reduction in digging behavior and an exacerbated hyperlocomotor response to amphetamine. Thus, targeted molecular analyses reveal an unforeseen heterogeneity in D2R-expressing striatal neuronal populations, underlying specific D2R’s functional features in the control of specific motor behaviors.
Fil: Puighermanal, Emma. Université Montpellier II; Francia. Universitat Autònoma de Barcelona; España. Centre National de la Recherche Scientifique; Francia. Inserm; Francia
Fil: Castell, Laia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Inserm; Francia
Fil: Esteve Codina, Anna. Barcelona Institute Of Science And Technology.; España
Fil: Melser, Su. Universite de Bordeaux; Francia. Inserm; Francia
Fil: Kaganovsky, Konstantin. University of Stanford; Estados Unidos
Fil: Zussy, Charleine. Inserm; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Boubaker Vitre, Jihane. Inserm; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gut, Marta. Barcelona Institute Of Science And Technology.; España. Universitat Pompeu Fabra; España
Fil: Rialle, Stephanie. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia. Inserm; Francia
Fil: Kellendonk, Christoph. Columbia University; Estados Unidos. New York State Psychiatric Institute; Estados Unidos
Fil: Sanz, Elisenda. Universitat Autònoma de Barcelona; España
Fil: Quintana, Albert. Universitat Autònoma de Barcelona; España
Fil: Marsicano, Giovanni. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Martin, Miquel. Inserm; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Rubinstein, Marcelo. University of Michigan; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Girault, Jean Antoine. Inserm; Francia. Institut du Fer à Moulin; Francia. Sorbonne University; Francia
Fil: Ding, Jun B.. University of Stanford; Estados Unidos
Fil: Valjent, Emmanuel. Inserm; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
Materia
DOPAMINE
DOPAMINE D2 RECEPTOR
RNASEQ
WSF1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/110735
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/110735
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatumPuighermanal, EmmaCastell, LaiaEsteve Codina, AnnaMelser, SuKaganovsky, KonstantinZussy, CharleineBoubaker Vitre, JihaneGut, MartaRialle, StephanieKellendonk, ChristophSanz, ElisendaQuintana, AlbertMarsicano, GiovanniMartin, MiquelRubinstein, MarceloGirault, Jean AntoineDing, Jun B.Valjent, EmmanuelDOPAMINEDOPAMINE D2 RECEPTORRNASEQWSF1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Action control is a key brain function determining the survival of animals in their environment. In mammals, neurons expressing dopamine D2 receptors (D2R) in the dorsal striatum (DS) and the nucleus accumbens (Acb) jointly but differentially contribute to the fine regulation of movement. However, their region-specific molecular features are presently unknown. By combining RNAseq of striatal D2R neurons and histological analyses, we identified hundreds of novel region-specific molecular markers, which may serve as tools to target selective subpopulations. As a proof of concept, we characterized the molecular identity of a subcircuit defined by WFS1 neurons and evaluated multiple behavioral tasks after its temporally-controlled deletion of D2R. Consequently, conditional D2R knockout mice displayed a significant reduction in digging behavior and an exacerbated hyperlocomotor response to amphetamine. Thus, targeted molecular analyses reveal an unforeseen heterogeneity in D2R-expressing striatal neuronal populations, underlying specific D2R’s functional features in the control of specific motor behaviors.Fil: Puighermanal, Emma. Université Montpellier II; Francia. Universitat Autònoma de Barcelona; España. Centre National de la Recherche Scientifique; Francia. Inserm; FranciaFil: Castell, Laia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Inserm; FranciaFil: Esteve Codina, Anna. Barcelona Institute Of Science And Technology.; EspañaFil: Melser, Su. Universite de Bordeaux; Francia. Inserm; FranciaFil: Kaganovsky, Konstantin. University of Stanford; Estados UnidosFil: Zussy, Charleine. Inserm; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Boubaker Vitre, Jihane. Inserm; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; FranciaFil: Gut, Marta. Barcelona Institute Of Science And Technology.; España. Universitat Pompeu Fabra; EspañaFil: Rialle, Stephanie. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia. Inserm; FranciaFil: Kellendonk, Christoph. Columbia University; Estados Unidos. New York State Psychiatric Institute; Estados UnidosFil: Sanz, Elisenda. Universitat Autònoma de Barcelona; EspañaFil: Quintana, Albert. Universitat Autònoma de Barcelona; EspañaFil: Marsicano, Giovanni. Inserm; Francia. Universite de Bordeaux; FranciaFil: Martin, Miquel. Inserm; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Rubinstein, Marcelo. University of Michigan; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Girault, Jean Antoine. Inserm; Francia. Institut du Fer à Moulin; Francia. Sorbonne University; FranciaFil: Ding, Jun B.. University of Stanford; Estados UnidosFil: Valjent, Emmanuel. Inserm; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; FranciaNature Publishing Group2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110735Puighermanal, Emma; Castell, Laia; Esteve Codina, Anna; Melser, Su; Kaganovsky, Konstantin; et al.; Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum; Nature Publishing Group; Nature Communications; 11; 1; 4-2020; 1-152041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41467-020-15716-9info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-020-15716-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:27Zoai:ri.conicet.gov.ar:11336/110735instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:27.619CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
title Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
spellingShingle Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
Puighermanal, Emma
DOPAMINE
DOPAMINE D2 RECEPTOR
RNASEQ
WSF1
title_short Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
title_full Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
title_fullStr Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
title_full_unstemmed Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
title_sort Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum
dc.creator.none.fl_str_mv Puighermanal, Emma
Castell, Laia
Esteve Codina, Anna
Melser, Su
Kaganovsky, Konstantin
Zussy, Charleine
Boubaker Vitre, Jihane
Gut, Marta
Rialle, Stephanie
Kellendonk, Christoph
Sanz, Elisenda
Quintana, Albert
Marsicano, Giovanni
Martin, Miquel
Rubinstein, Marcelo
Girault, Jean Antoine
Ding, Jun B.
Valjent, Emmanuel
author Puighermanal, Emma
author_facet Puighermanal, Emma
Castell, Laia
Esteve Codina, Anna
Melser, Su
Kaganovsky, Konstantin
Zussy, Charleine
Boubaker Vitre, Jihane
Gut, Marta
Rialle, Stephanie
Kellendonk, Christoph
Sanz, Elisenda
Quintana, Albert
Marsicano, Giovanni
Martin, Miquel
Rubinstein, Marcelo
Girault, Jean Antoine
Ding, Jun B.
Valjent, Emmanuel
author_role author
author2 Castell, Laia
Esteve Codina, Anna
Melser, Su
Kaganovsky, Konstantin
Zussy, Charleine
Boubaker Vitre, Jihane
Gut, Marta
Rialle, Stephanie
Kellendonk, Christoph
Sanz, Elisenda
Quintana, Albert
Marsicano, Giovanni
Martin, Miquel
Rubinstein, Marcelo
Girault, Jean Antoine
Ding, Jun B.
Valjent, Emmanuel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DOPAMINE
DOPAMINE D2 RECEPTOR
RNASEQ
WSF1
topic DOPAMINE
DOPAMINE D2 RECEPTOR
RNASEQ
WSF1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Action control is a key brain function determining the survival of animals in their environment. In mammals, neurons expressing dopamine D2 receptors (D2R) in the dorsal striatum (DS) and the nucleus accumbens (Acb) jointly but differentially contribute to the fine regulation of movement. However, their region-specific molecular features are presently unknown. By combining RNAseq of striatal D2R neurons and histological analyses, we identified hundreds of novel region-specific molecular markers, which may serve as tools to target selective subpopulations. As a proof of concept, we characterized the molecular identity of a subcircuit defined by WFS1 neurons and evaluated multiple behavioral tasks after its temporally-controlled deletion of D2R. Consequently, conditional D2R knockout mice displayed a significant reduction in digging behavior and an exacerbated hyperlocomotor response to amphetamine. Thus, targeted molecular analyses reveal an unforeseen heterogeneity in D2R-expressing striatal neuronal populations, underlying specific D2R’s functional features in the control of specific motor behaviors.
Fil: Puighermanal, Emma. Université Montpellier II; Francia. Universitat Autònoma de Barcelona; España. Centre National de la Recherche Scientifique; Francia. Inserm; Francia
Fil: Castell, Laia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Inserm; Francia
Fil: Esteve Codina, Anna. Barcelona Institute Of Science And Technology.; España
Fil: Melser, Su. Universite de Bordeaux; Francia. Inserm; Francia
Fil: Kaganovsky, Konstantin. University of Stanford; Estados Unidos
Fil: Zussy, Charleine. Inserm; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Boubaker Vitre, Jihane. Inserm; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gut, Marta. Barcelona Institute Of Science And Technology.; España. Universitat Pompeu Fabra; España
Fil: Rialle, Stephanie. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia. Inserm; Francia
Fil: Kellendonk, Christoph. Columbia University; Estados Unidos. New York State Psychiatric Institute; Estados Unidos
Fil: Sanz, Elisenda. Universitat Autònoma de Barcelona; España
Fil: Quintana, Albert. Universitat Autònoma de Barcelona; España
Fil: Marsicano, Giovanni. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Martin, Miquel. Inserm; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Rubinstein, Marcelo. University of Michigan; Estados Unidos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Girault, Jean Antoine. Inserm; Francia. Institut du Fer à Moulin; Francia. Sorbonne University; Francia
Fil: Ding, Jun B.. University of Stanford; Estados Unidos
Fil: Valjent, Emmanuel. Inserm; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
description Action control is a key brain function determining the survival of animals in their environment. In mammals, neurons expressing dopamine D2 receptors (D2R) in the dorsal striatum (DS) and the nucleus accumbens (Acb) jointly but differentially contribute to the fine regulation of movement. However, their region-specific molecular features are presently unknown. By combining RNAseq of striatal D2R neurons and histological analyses, we identified hundreds of novel region-specific molecular markers, which may serve as tools to target selective subpopulations. As a proof of concept, we characterized the molecular identity of a subcircuit defined by WFS1 neurons and evaluated multiple behavioral tasks after its temporally-controlled deletion of D2R. Consequently, conditional D2R knockout mice displayed a significant reduction in digging behavior and an exacerbated hyperlocomotor response to amphetamine. Thus, targeted molecular analyses reveal an unforeseen heterogeneity in D2R-expressing striatal neuronal populations, underlying specific D2R’s functional features in the control of specific motor behaviors.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/110735
Puighermanal, Emma; Castell, Laia; Esteve Codina, Anna; Melser, Su; Kaganovsky, Konstantin; et al.; Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum; Nature Publishing Group; Nature Communications; 11; 1; 4-2020; 1-15
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/110735
identifier_str_mv Puighermanal, Emma; Castell, Laia; Esteve Codina, Anna; Melser, Su; Kaganovsky, Konstantin; et al.; Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum; Nature Publishing Group; Nature Communications; 11; 1; 4-2020; 1-15
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41467-020-15716-9
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-020-15716-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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