Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes

Autores
Van Gough, Dara; Wolosiuk, Alejandro; Braun, Paul V.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules larger than the mesopores, which could not pass into the hollow sphere, did not interact with the SBP. Specifically, encapsulated SBP catalyzed the oxidation of Amplex Ultra-Red, a small fluorogen, in the presence of hydrogen peroxide, encapsulated SBP was deactivated by sodium azide, and no reaction was observed between encapsulated SBP and a greater than 3 nm diameter protease.
Fil: Van Gough, Dara. University of Illinois; Estados Unidos
Fil: Wolosiuk, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina. Universidad de Buenos Aires; Argentina
Fil: Braun, Paul V.. University of Illinois; Estados Unidos
Materia
COLLOIDS
ENCAPSULATION
FLUORESCENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/245041
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/245041
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated EnzymesVan Gough, DaraWolosiuk, AlejandroBraun, Paul V.COLLOIDSENCAPSULATIONFLUORESCENCEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules larger than the mesopores, which could not pass into the hollow sphere, did not interact with the SBP. Specifically, encapsulated SBP catalyzed the oxidation of Amplex Ultra-Red, a small fluorogen, in the presence of hydrogen peroxide, encapsulated SBP was deactivated by sodium azide, and no reaction was observed between encapsulated SBP and a greater than 3 nm diameter protease.Fil: Van Gough, Dara. University of Illinois; Estados UnidosFil: Wolosiuk, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina. Universidad de Buenos Aires; ArgentinaFil: Braun, Paul V.. University of Illinois; Estados UnidosAmerican Chemical Society2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/245041Van Gough, Dara; Wolosiuk, Alejandro; Braun, Paul V.; Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes; American Chemical Society; Nano Letters; 9; 5; 12-2009; 1994-19981530-6984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/nl900264ninfo:eu-repo/semantics/altIdentifier/doi/10.1021/nl900264ninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:17:16Zoai:ri.conicet.gov.ar:11336/245041instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:17:16.706CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
title Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
spellingShingle Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
Van Gough, Dara
COLLOIDS
ENCAPSULATION
FLUORESCENCE
title_short Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
title_full Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
title_fullStr Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
title_full_unstemmed Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
title_sort Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes
dc.creator.none.fl_str_mv Van Gough, Dara
Wolosiuk, Alejandro
Braun, Paul V.
author Van Gough, Dara
author_facet Van Gough, Dara
Wolosiuk, Alejandro
Braun, Paul V.
author_role author
author2 Wolosiuk, Alejandro
Braun, Paul V.
author2_role author
author
dc.subject.none.fl_str_mv COLLOIDS
ENCAPSULATION
FLUORESCENCE
topic COLLOIDS
ENCAPSULATION
FLUORESCENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules larger than the mesopores, which could not pass into the hollow sphere, did not interact with the SBP. Specifically, encapsulated SBP catalyzed the oxidation of Amplex Ultra-Red, a small fluorogen, in the presence of hydrogen peroxide, encapsulated SBP was deactivated by sodium azide, and no reaction was observed between encapsulated SBP and a greater than 3 nm diameter protease.
Fil: Van Gough, Dara. University of Illinois; Estados Unidos
Fil: Wolosiuk, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina. Universidad de Buenos Aires; Argentina
Fil: Braun, Paul V.. University of Illinois; Estados Unidos
description A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules larger than the mesopores, which could not pass into the hollow sphere, did not interact with the SBP. Specifically, encapsulated SBP catalyzed the oxidation of Amplex Ultra-Red, a small fluorogen, in the presence of hydrogen peroxide, encapsulated SBP was deactivated by sodium azide, and no reaction was observed between encapsulated SBP and a greater than 3 nm diameter protease.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/245041
Van Gough, Dara; Wolosiuk, Alejandro; Braun, Paul V.; Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes; American Chemical Society; Nano Letters; 9; 5; 12-2009; 1994-1998
1530-6984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/245041
identifier_str_mv Van Gough, Dara; Wolosiuk, Alejandro; Braun, Paul V.; Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes; American Chemical Society; Nano Letters; 9; 5; 12-2009; 1994-1998
1530-6984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/nl900264n
info:eu-repo/semantics/altIdentifier/doi/10.1021/nl900264n
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847426074049249280
score 13.10058

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