Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles

Autores
Fölling, Jonas; Polyakova, Svetlana; Belov, Vladimir; Van Blaaderen, Alfons; Bossi, Mariano Luis; Hell, Stefan W.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS -N-hydroxysuccinimide ester- allows selective labeling of aminocontaining molecules or other materials. In ethanolic solutions, thecompound displays a large fluorescent quantum yield of 52% and alarge fluorescence modulation ratio (94%) between two states that maybe interconverted withred and near-UV light. Silica nanoparticles incorporatingthe new FMS were prepared and characterized, and their spectroscopicand switching properties were also studied. The dye retained itsproperties after the incorporation into the silica, thereby allowing lightinducedreversible high modulation of the fluorescence signal of a singleparticle for up to 60 cycles, before undergoing irreversible photobleaching.Some applications of these particles in fluorescence microscopy arealso demonstrated. In particular, subdiffraction images of nanoparticleswere obtained, in the focal plane of a confocal microscope.
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Polyakova, Svetlana. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Van Blaaderen, Alfons. Utrecht University; Países Bajos
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hell, Stefan W.. Utrecht University; Países Bajos
Materia
colloids
fluorescence microscopy
molecular switches
photochromism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102995
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102995
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and Characterization of Photoswitchable Fluorescent Silica NanoparticlesFölling, JonasPolyakova, SvetlanaBelov, VladimirVan Blaaderen, AlfonsBossi, Mariano LuisHell, Stefan W.colloidsfluorescence microscopymolecular switchesphotochromismhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS -N-hydroxysuccinimide ester- allows selective labeling of aminocontaining molecules or other materials. In ethanolic solutions, thecompound displays a large fluorescent quantum yield of 52% and alarge fluorescence modulation ratio (94%) between two states that maybe interconverted withred and near-UV light. Silica nanoparticles incorporatingthe new FMS were prepared and characterized, and their spectroscopicand switching properties were also studied. The dye retained itsproperties after the incorporation into the silica, thereby allowing lightinducedreversible high modulation of the fluorescence signal of a singleparticle for up to 60 cycles, before undergoing irreversible photobleaching.Some applications of these particles in fluorescence microscopy arealso demonstrated. In particular, subdiffraction images of nanoparticleswere obtained, in the focal plane of a confocal microscope.Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Polyakova, Svetlana. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Van Blaaderen, Alfons. Utrecht University; Países BajosFil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hell, Stefan W.. Utrecht University; Países BajosWiley VCH Verlag2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102995Fölling, Jonas; Polyakova, Svetlana; Belov, Vladimir; Van Blaaderen, Alfons; Bossi, Mariano Luis; et al.; Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles; Wiley VCH Verlag; Small; 4; 1; 1-2008; 134-1421613-6810CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.200700440info:eu-repo/semantics/altIdentifier/doi/10.1002/smll.200700440info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:18Zoai:ri.conicet.gov.ar:11336/102995instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:18.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
title Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
spellingShingle Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
Fölling, Jonas
colloids
fluorescence microscopy
molecular switches
photochromism
title_short Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
title_full Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
title_fullStr Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
title_full_unstemmed Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
title_sort Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
dc.creator.none.fl_str_mv Fölling, Jonas
Polyakova, Svetlana
Belov, Vladimir
Van Blaaderen, Alfons
Bossi, Mariano Luis
Hell, Stefan W.
author Fölling, Jonas
author_facet Fölling, Jonas
Polyakova, Svetlana
Belov, Vladimir
Van Blaaderen, Alfons
Bossi, Mariano Luis
Hell, Stefan W.
author_role author
author2 Polyakova, Svetlana
Belov, Vladimir
Van Blaaderen, Alfons
Bossi, Mariano Luis
Hell, Stefan W.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv colloids
fluorescence microscopy
molecular switches
photochromism
topic colloids
fluorescence microscopy
molecular switches
photochromism
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS -N-hydroxysuccinimide ester- allows selective labeling of aminocontaining molecules or other materials. In ethanolic solutions, thecompound displays a large fluorescent quantum yield of 52% and alarge fluorescence modulation ratio (94%) between two states that maybe interconverted withred and near-UV light. Silica nanoparticles incorporatingthe new FMS were prepared and characterized, and their spectroscopicand switching properties were also studied. The dye retained itsproperties after the incorporation into the silica, thereby allowing lightinducedreversible high modulation of the fluorescence signal of a singleparticle for up to 60 cycles, before undergoing irreversible photobleaching.Some applications of these particles in fluorescence microscopy arealso demonstrated. In particular, subdiffraction images of nanoparticleswere obtained, in the focal plane of a confocal microscope.
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Polyakova, Svetlana. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Van Blaaderen, Alfons. Utrecht University; Países Bajos
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hell, Stefan W.. Utrecht University; Países Bajos
description We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS -N-hydroxysuccinimide ester- allows selective labeling of aminocontaining molecules or other materials. In ethanolic solutions, thecompound displays a large fluorescent quantum yield of 52% and alarge fluorescence modulation ratio (94%) between two states that maybe interconverted withred and near-UV light. Silica nanoparticles incorporatingthe new FMS were prepared and characterized, and their spectroscopicand switching properties were also studied. The dye retained itsproperties after the incorporation into the silica, thereby allowing lightinducedreversible high modulation of the fluorescence signal of a singleparticle for up to 60 cycles, before undergoing irreversible photobleaching.Some applications of these particles in fluorescence microscopy arealso demonstrated. In particular, subdiffraction images of nanoparticleswere obtained, in the focal plane of a confocal microscope.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102995
Fölling, Jonas; Polyakova, Svetlana; Belov, Vladimir; Van Blaaderen, Alfons; Bossi, Mariano Luis; et al.; Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles; Wiley VCH Verlag; Small; 4; 1; 1-2008; 134-142
1613-6810
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102995
identifier_str_mv Fölling, Jonas; Polyakova, Svetlana; Belov, Vladimir; Van Blaaderen, Alfons; Bossi, Mariano Luis; et al.; Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles; Wiley VCH Verlag; Small; 4; 1; 1-2008; 134-142
1613-6810
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.200700440
info:eu-repo/semantics/altIdentifier/doi/10.1002/smll.200700440
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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