Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina

Autores
Acardi, Soraya Alejandra; Rago, María Virginia; Liotta, Domingo Javier; Fernandez Duque, Eduardo; Salomon, Oscar Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
American Cutaneous Leishmaniasis (ACL) is caused by protozoan parasites of the Leishmania genus, and transmitted by females of the Phlebotominae family. The role of wild and domestic hosts in the cycle of Leishmania is still unknown. ACL is endemic in the province of Formosa where Nyssomyia neivai was the most abundant species in several captures and 31 cumulative ACL human cases were reported between 2005 and 2011 in the province. The present report describes the detection, by PCR-RFLP and confirmed by sequencing, of subgenus Leishmania (Viannia) DNA in four free-ranging owl monkeys (Aotus azarai azarai) from Formosa Province. The sequence amplified was the mini-exon gene present in tandem repeats in all species of the Leishmania genus from buffy coat samples. The absence of inhibitors in the samples was checked by a β-globin protocol originally designed to amplify the human β-globin gene. However, other free-ranging primates were found with natural infections of L. (V) braziliensis complex and Leishmania (Viannia) subgenus by parasitological means in America. To the best of our knowledge, there are no published reports on detection of subgenus Leishmania (Viannia) DNA by PCR-RFLP in argentinean free-ranging primates. Additional eco-epidemiological and parasitological studies are necessary to confirm owl monkeys, or any other natural infected mammal species detected by PCR, as a reservoir, incidental host or to propose it as an animal model for research on this topic.
Fil: Acardi, Soraya Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Rago, María Virginia. Wildlife Conservation Society; Estados Unidos
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Centro de Ecologia Aplicada del Litoral (i); Argentina. University of Pennsylvania; Estados Unidos
Fil: Salomon, Oscar Daniel. Instituto Nacional de Medicina Tropical, Misiones; Argentina
Materia
Leishmania
Dna
Primates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7554
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, ArgentinaAcardi, Soraya AlejandraRago, María VirginiaLiotta, Domingo JavierFernandez Duque, EduardoSalomon, Oscar DanielLeishmaniaDnaPrimateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1American Cutaneous Leishmaniasis (ACL) is caused by protozoan parasites of the Leishmania genus, and transmitted by females of the Phlebotominae family. The role of wild and domestic hosts in the cycle of Leishmania is still unknown. ACL is endemic in the province of Formosa where Nyssomyia neivai was the most abundant species in several captures and 31 cumulative ACL human cases were reported between 2005 and 2011 in the province. The present report describes the detection, by PCR-RFLP and confirmed by sequencing, of subgenus Leishmania (Viannia) DNA in four free-ranging owl monkeys (Aotus azarai azarai) from Formosa Province. The sequence amplified was the mini-exon gene present in tandem repeats in all species of the Leishmania genus from buffy coat samples. The absence of inhibitors in the samples was checked by a β-globin protocol originally designed to amplify the human β-globin gene. However, other free-ranging primates were found with natural infections of L. (V) braziliensis complex and Leishmania (Viannia) subgenus by parasitological means in America. To the best of our knowledge, there are no published reports on detection of subgenus Leishmania (Viannia) DNA by PCR-RFLP in argentinean free-ranging primates. Additional eco-epidemiological and parasitological studies are necessary to confirm owl monkeys, or any other natural infected mammal species detected by PCR, as a reservoir, incidental host or to propose it as an animal model for research on this topic.Fil: Acardi, Soraya Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Rago, María Virginia. Wildlife Conservation Society; Estados UnidosFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Centro de Ecologia Aplicada del Litoral (i); Argentina. University of Pennsylvania; Estados UnidosFil: Salomon, Oscar Daniel. Instituto Nacional de Medicina Tropical, Misiones; ArgentinaElsevier Science2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7554Acardi, Soraya Alejandra; Rago, María Virginia; Liotta, Domingo Javier; Fernandez Duque, Eduardo; Salomon, Oscar Daniel; Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina; Elsevier Science; Veterinary Parasitology; 193; 1-3; 3-2013; 256-2590304-4017enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401712006607info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2012.12.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:20Zoai:ri.conicet.gov.ar:11336/7554instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:21.071CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
title Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
spellingShingle Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
Acardi, Soraya Alejandra
Leishmania
Dna
Primates
title_short Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
title_full Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
title_fullStr Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
title_full_unstemmed Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
title_sort Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina
dc.creator.none.fl_str_mv Acardi, Soraya Alejandra
Rago, María Virginia
Liotta, Domingo Javier
Fernandez Duque, Eduardo
Salomon, Oscar Daniel
author Acardi, Soraya Alejandra
author_facet Acardi, Soraya Alejandra
Rago, María Virginia
Liotta, Domingo Javier
Fernandez Duque, Eduardo
Salomon, Oscar Daniel
author_role author
author2 Rago, María Virginia
Liotta, Domingo Javier
Fernandez Duque, Eduardo
Salomon, Oscar Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Leishmania
Dna
Primates
topic Leishmania
Dna
Primates
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv American Cutaneous Leishmaniasis (ACL) is caused by protozoan parasites of the Leishmania genus, and transmitted by females of the Phlebotominae family. The role of wild and domestic hosts in the cycle of Leishmania is still unknown. ACL is endemic in the province of Formosa where Nyssomyia neivai was the most abundant species in several captures and 31 cumulative ACL human cases were reported between 2005 and 2011 in the province. The present report describes the detection, by PCR-RFLP and confirmed by sequencing, of subgenus Leishmania (Viannia) DNA in four free-ranging owl monkeys (Aotus azarai azarai) from Formosa Province. The sequence amplified was the mini-exon gene present in tandem repeats in all species of the Leishmania genus from buffy coat samples. The absence of inhibitors in the samples was checked by a β-globin protocol originally designed to amplify the human β-globin gene. However, other free-ranging primates were found with natural infections of L. (V) braziliensis complex and Leishmania (Viannia) subgenus by parasitological means in America. To the best of our knowledge, there are no published reports on detection of subgenus Leishmania (Viannia) DNA by PCR-RFLP in argentinean free-ranging primates. Additional eco-epidemiological and parasitological studies are necessary to confirm owl monkeys, or any other natural infected mammal species detected by PCR, as a reservoir, incidental host or to propose it as an animal model for research on this topic.
Fil: Acardi, Soraya Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Rago, María Virginia. Wildlife Conservation Society; Estados Unidos
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Centro de Ecologia Aplicada del Litoral (i); Argentina. University of Pennsylvania; Estados Unidos
Fil: Salomon, Oscar Daniel. Instituto Nacional de Medicina Tropical, Misiones; Argentina
description American Cutaneous Leishmaniasis (ACL) is caused by protozoan parasites of the Leishmania genus, and transmitted by females of the Phlebotominae family. The role of wild and domestic hosts in the cycle of Leishmania is still unknown. ACL is endemic in the province of Formosa where Nyssomyia neivai was the most abundant species in several captures and 31 cumulative ACL human cases were reported between 2005 and 2011 in the province. The present report describes the detection, by PCR-RFLP and confirmed by sequencing, of subgenus Leishmania (Viannia) DNA in four free-ranging owl monkeys (Aotus azarai azarai) from Formosa Province. The sequence amplified was the mini-exon gene present in tandem repeats in all species of the Leishmania genus from buffy coat samples. The absence of inhibitors in the samples was checked by a β-globin protocol originally designed to amplify the human β-globin gene. However, other free-ranging primates were found with natural infections of L. (V) braziliensis complex and Leishmania (Viannia) subgenus by parasitological means in America. To the best of our knowledge, there are no published reports on detection of subgenus Leishmania (Viannia) DNA by PCR-RFLP in argentinean free-ranging primates. Additional eco-epidemiological and parasitological studies are necessary to confirm owl monkeys, or any other natural infected mammal species detected by PCR, as a reservoir, incidental host or to propose it as an animal model for research on this topic.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7554
Acardi, Soraya Alejandra; Rago, María Virginia; Liotta, Domingo Javier; Fernandez Duque, Eduardo; Salomon, Oscar Daniel; Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina; Elsevier Science; Veterinary Parasitology; 193; 1-3; 3-2013; 256-259
0304-4017
url http://hdl.handle.net/11336/7554
identifier_str_mv Acardi, Soraya Alejandra; Rago, María Virginia; Liotta, Domingo Javier; Fernandez Duque, Eduardo; Salomon, Oscar Daniel; Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina; Elsevier Science; Veterinary Parasitology; 193; 1-3; 3-2013; 256-259
0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401712006607
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2012.12.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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