An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)

Autores
Babb, Paul L.; McIntosh, Annick M.; Fernandez Duque, Eduardo; Di Fiore, Anthony; Schurr, Theodore G.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we characterize a panel of 20 microsatellite markers that reproducibly amplify in Azara’s owl monkeys (Aotus azarai) for use in genetic profiling analyses. A total of 128 individuals from our study site in Formosa, Argentina, were genotyped for 20 markers, 13 of which were found to be polymorphic. The levels of allelic variation at these loci provided paternity exclusion probabilities of 0.852 when neither parent was known, and 0.981 when one parent was known. In addition, our analysis revealed that, although genotypes can be rapidly scored using fluorescence-based fragment analysis, the presence of complex or multiple short tandem repeat (STR) motifs at a microsatellite locus could generate similar fragment patterns from alleles that have different nucleotide sequences and perhaps different evolutionary origins. Even so, this collection of microsatellite loci is suitable for parentage analyses and will allow us to test various hypotheses about the relationship between social behavior and kinship in wild owl monkey populations. Furthermore, given the limited number of platyrrhine-specific microsatellite loci available in the literature, this STR panel represents a valuable tool for population studies of other cebines and callitrichines.
Fil: Babb, Paul L.. University of Pennsylvania; Estados Unidos
Fil: McIntosh, Annick M.. Haverford College; Estados Unidos
Fil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina. University of Pennsylvania; Estados Unidos
Fil: Di Fiore, Anthony. University of Texas at Austin; Estados Unidos
Fil: Schurr, Theodore G.. University of Pennsylvania; Estados Unidos
Materia
Primates
Platyrrhines
Monogamy
Short Tandem Beats
Autosomal Dna
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101258
Acceder
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spelling An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)Babb, Paul L.McIntosh, Annick M.Fernandez Duque, EduardoDi Fiore, AnthonySchurr, Theodore G.PrimatesPlatyrrhinesMonogamyShort Tandem BeatsAutosomal Dnahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, we characterize a panel of 20 microsatellite markers that reproducibly amplify in Azara’s owl monkeys (Aotus azarai) for use in genetic profiling analyses. A total of 128 individuals from our study site in Formosa, Argentina, were genotyped for 20 markers, 13 of which were found to be polymorphic. The levels of allelic variation at these loci provided paternity exclusion probabilities of 0.852 when neither parent was known, and 0.981 when one parent was known. In addition, our analysis revealed that, although genotypes can be rapidly scored using fluorescence-based fragment analysis, the presence of complex or multiple short tandem repeat (STR) motifs at a microsatellite locus could generate similar fragment patterns from alleles that have different nucleotide sequences and perhaps different evolutionary origins. Even so, this collection of microsatellite loci is suitable for parentage analyses and will allow us to test various hypotheses about the relationship between social behavior and kinship in wild owl monkey populations. Furthermore, given the limited number of platyrrhine-specific microsatellite loci available in the literature, this STR panel represents a valuable tool for population studies of other cebines and callitrichines.Fil: Babb, Paul L.. University of Pennsylvania; Estados UnidosFil: McIntosh, Annick M.. Haverford College; Estados UnidosFil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina. University of Pennsylvania; Estados UnidosFil: Di Fiore, Anthony. University of Texas at Austin; Estados UnidosFil: Schurr, Theodore G.. University of Pennsylvania; Estados UnidosKarger2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101258Babb, Paul L.; McIntosh, Annick M.; Fernandez Duque, Eduardo; Di Fiore, Anthony; Schurr, Theodore G.; An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai); Karger; Folia Primatologica; 82; 2; 9-2011; 107-1170015-5713CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000330564info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/330564info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:34Zoai:ri.conicet.gov.ar:11336/101258instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:34.547CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
title An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
spellingShingle An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
Babb, Paul L.
Primates
Platyrrhines
Monogamy
Short Tandem Beats
Autosomal Dna
title_short An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
title_full An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
title_fullStr An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
title_full_unstemmed An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
title_sort An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai)
dc.creator.none.fl_str_mv Babb, Paul L.
McIntosh, Annick M.
Fernandez Duque, Eduardo
Di Fiore, Anthony
Schurr, Theodore G.
author Babb, Paul L.
author_facet Babb, Paul L.
McIntosh, Annick M.
Fernandez Duque, Eduardo
Di Fiore, Anthony
Schurr, Theodore G.
author_role author
author2 McIntosh, Annick M.
Fernandez Duque, Eduardo
Di Fiore, Anthony
Schurr, Theodore G.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Primates
Platyrrhines
Monogamy
Short Tandem Beats
Autosomal Dna
topic Primates
Platyrrhines
Monogamy
Short Tandem Beats
Autosomal Dna
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, we characterize a panel of 20 microsatellite markers that reproducibly amplify in Azara’s owl monkeys (Aotus azarai) for use in genetic profiling analyses. A total of 128 individuals from our study site in Formosa, Argentina, were genotyped for 20 markers, 13 of which were found to be polymorphic. The levels of allelic variation at these loci provided paternity exclusion probabilities of 0.852 when neither parent was known, and 0.981 when one parent was known. In addition, our analysis revealed that, although genotypes can be rapidly scored using fluorescence-based fragment analysis, the presence of complex or multiple short tandem repeat (STR) motifs at a microsatellite locus could generate similar fragment patterns from alleles that have different nucleotide sequences and perhaps different evolutionary origins. Even so, this collection of microsatellite loci is suitable for parentage analyses and will allow us to test various hypotheses about the relationship between social behavior and kinship in wild owl monkey populations. Furthermore, given the limited number of platyrrhine-specific microsatellite loci available in the literature, this STR panel represents a valuable tool for population studies of other cebines and callitrichines.
Fil: Babb, Paul L.. University of Pennsylvania; Estados Unidos
Fil: McIntosh, Annick M.. Haverford College; Estados Unidos
Fil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina. University of Pennsylvania; Estados Unidos
Fil: Di Fiore, Anthony. University of Texas at Austin; Estados Unidos
Fil: Schurr, Theodore G.. University of Pennsylvania; Estados Unidos
description In this study, we characterize a panel of 20 microsatellite markers that reproducibly amplify in Azara’s owl monkeys (Aotus azarai) for use in genetic profiling analyses. A total of 128 individuals from our study site in Formosa, Argentina, were genotyped for 20 markers, 13 of which were found to be polymorphic. The levels of allelic variation at these loci provided paternity exclusion probabilities of 0.852 when neither parent was known, and 0.981 when one parent was known. In addition, our analysis revealed that, although genotypes can be rapidly scored using fluorescence-based fragment analysis, the presence of complex or multiple short tandem repeat (STR) motifs at a microsatellite locus could generate similar fragment patterns from alleles that have different nucleotide sequences and perhaps different evolutionary origins. Even so, this collection of microsatellite loci is suitable for parentage analyses and will allow us to test various hypotheses about the relationship between social behavior and kinship in wild owl monkey populations. Furthermore, given the limited number of platyrrhine-specific microsatellite loci available in the literature, this STR panel represents a valuable tool for population studies of other cebines and callitrichines.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101258
Babb, Paul L.; McIntosh, Annick M.; Fernandez Duque, Eduardo; Di Fiore, Anthony; Schurr, Theodore G.; An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai); Karger; Folia Primatologica; 82; 2; 9-2011; 107-117
0015-5713
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101258
identifier_str_mv Babb, Paul L.; McIntosh, Annick M.; Fernandez Duque, Eduardo; Di Fiore, Anthony; Schurr, Theodore G.; An Optimized Microsatellite Genotyping Strategy for Assessing Genetic Identity and Kinship in Azara's Owl Monkeys (Aotus azarai); Karger; Folia Primatologica; 82; 2; 9-2011; 107-117
0015-5713
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000330564
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/330564
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.004268

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