Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3

Autores
Piguillem Palacios, Sofía Viviana; María Gamella; García de Frutos, Pablo; Batlle, Montserrat; Yáñez Sedeño, Paloma; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Campuzano, Susana; Pedrero, María; Pingarrón, José M.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the first electrochemical immunoassay involving magnetic microbeads (MBs) for the determination of galectin-3 (Gal-3), a β-galactosidase-binding lectin that acts as mediator of heart failure (HF). MBs-captured sandwich-type immune complexes and amperometric detection at disposable screen-printed carbon electrodes were used. The immunoplatform showed a detection limit of 8.3 pg mL−1, good reproducibility, and excellent selectivity. The endogenous concentration of Gal-3 in human plasma from HF patients was determined with results in agreement with those obtained using ELISA. The multiplexing feasibility of the developed immunoplatform was demonstrated for the simultaneous determination of Gal-3 and N-terminal pro-brain natriuretic peptide (NT-proBNP).
Fil: Piguillem Palacios, Sofía Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Complutense de Madrid; España
Fil: María Gamella. Universidad Complutense de Madrid; España
Fil: García de Frutos, Pablo. INSTITUTO DE INVESTIGACIONES BIOMEDICAS AUGUST PI I SUNYER (IDIBAPS);
Fil: Batlle, Montserrat. No especifíca;
Fil: Yáñez Sedeño, Paloma. Universidad Complutense de Madrid; España
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Campuzano, Susana. Universidad Complutense de Madrid; España
Fil: Pedrero, María. Universidad Complutense de Madrid; España
Fil: Pingarrón, José M.. Universidad Complutense de Madrid; España
Materia
ELECTROCHEMICAL IMMUNOPLATFORM
GALECTIN-3
HEART FAILURE
MAGNETIC BEADS
PLASMA SAMPLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138544
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/138544
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3Piguillem Palacios, Sofía VivianaMaría GamellaGarcía de Frutos, PabloBatlle, MontserratYáñez Sedeño, PalomaMessina, Germán AlejandroFernández Baldo, Martín AlejandroCampuzano, SusanaPedrero, MaríaPingarrón, José M.ELECTROCHEMICAL IMMUNOPLATFORMGALECTIN-3HEART FAILUREMAGNETIC BEADSPLASMA SAMPLEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work reports the first electrochemical immunoassay involving magnetic microbeads (MBs) for the determination of galectin-3 (Gal-3), a β-galactosidase-binding lectin that acts as mediator of heart failure (HF). MBs-captured sandwich-type immune complexes and amperometric detection at disposable screen-printed carbon electrodes were used. The immunoplatform showed a detection limit of 8.3 pg mL−1, good reproducibility, and excellent selectivity. The endogenous concentration of Gal-3 in human plasma from HF patients was determined with results in agreement with those obtained using ELISA. The multiplexing feasibility of the developed immunoplatform was demonstrated for the simultaneous determination of Gal-3 and N-terminal pro-brain natriuretic peptide (NT-proBNP).Fil: Piguillem Palacios, Sofía Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Complutense de Madrid; EspañaFil: María Gamella. Universidad Complutense de Madrid; EspañaFil: García de Frutos, Pablo. INSTITUTO DE INVESTIGACIONES BIOMEDICAS AUGUST PI I SUNYER (IDIBAPS);Fil: Batlle, Montserrat. No especifíca;Fil: Yáñez Sedeño, Paloma. Universidad Complutense de Madrid; EspañaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Campuzano, Susana. Universidad Complutense de Madrid; EspañaFil: Pedrero, María. Universidad Complutense de Madrid; EspañaFil: Pingarrón, José M.. Universidad Complutense de Madrid; EspañaWiley VCH Verlag2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138544Piguillem Palacios, Sofía Viviana; María Gamella; García de Frutos, Pablo; Batlle, Montserrat; Yáñez Sedeño, Paloma; et al.; Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3; Wiley VCH Verlag; Electroanalysis; 32; 12; 8-2020; 2775-27851040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elan.202060323info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.202060323info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:17:39Zoai:ri.conicet.gov.ar:11336/138544instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:17:39.526CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
title Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
spellingShingle Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
Piguillem Palacios, Sofía Viviana
ELECTROCHEMICAL IMMUNOPLATFORM
GALECTIN-3
HEART FAILURE
MAGNETIC BEADS
PLASMA SAMPLES
title_short Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
title_full Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
title_fullStr Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
title_full_unstemmed Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
title_sort Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3
dc.creator.none.fl_str_mv Piguillem Palacios, Sofía Viviana
María Gamella
García de Frutos, Pablo
Batlle, Montserrat
Yáñez Sedeño, Paloma
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
Campuzano, Susana
Pedrero, María
Pingarrón, José M.
author Piguillem Palacios, Sofía Viviana
author_facet Piguillem Palacios, Sofía Viviana
María Gamella
García de Frutos, Pablo
Batlle, Montserrat
Yáñez Sedeño, Paloma
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
Campuzano, Susana
Pedrero, María
Pingarrón, José M.
author_role author
author2 María Gamella
García de Frutos, Pablo
Batlle, Montserrat
Yáñez Sedeño, Paloma
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
Campuzano, Susana
Pedrero, María
Pingarrón, José M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ELECTROCHEMICAL IMMUNOPLATFORM
GALECTIN-3
HEART FAILURE
MAGNETIC BEADS
PLASMA SAMPLES
topic ELECTROCHEMICAL IMMUNOPLATFORM
GALECTIN-3
HEART FAILURE
MAGNETIC BEADS
PLASMA SAMPLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work reports the first electrochemical immunoassay involving magnetic microbeads (MBs) for the determination of galectin-3 (Gal-3), a β-galactosidase-binding lectin that acts as mediator of heart failure (HF). MBs-captured sandwich-type immune complexes and amperometric detection at disposable screen-printed carbon electrodes were used. The immunoplatform showed a detection limit of 8.3 pg mL−1, good reproducibility, and excellent selectivity. The endogenous concentration of Gal-3 in human plasma from HF patients was determined with results in agreement with those obtained using ELISA. The multiplexing feasibility of the developed immunoplatform was demonstrated for the simultaneous determination of Gal-3 and N-terminal pro-brain natriuretic peptide (NT-proBNP).
Fil: Piguillem Palacios, Sofía Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Complutense de Madrid; España
Fil: María Gamella. Universidad Complutense de Madrid; España
Fil: García de Frutos, Pablo. INSTITUTO DE INVESTIGACIONES BIOMEDICAS AUGUST PI I SUNYER (IDIBAPS);
Fil: Batlle, Montserrat. No especifíca;
Fil: Yáñez Sedeño, Paloma. Universidad Complutense de Madrid; España
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Campuzano, Susana. Universidad Complutense de Madrid; España
Fil: Pedrero, María. Universidad Complutense de Madrid; España
Fil: Pingarrón, José M.. Universidad Complutense de Madrid; España
description This work reports the first electrochemical immunoassay involving magnetic microbeads (MBs) for the determination of galectin-3 (Gal-3), a β-galactosidase-binding lectin that acts as mediator of heart failure (HF). MBs-captured sandwich-type immune complexes and amperometric detection at disposable screen-printed carbon electrodes were used. The immunoplatform showed a detection limit of 8.3 pg mL−1, good reproducibility, and excellent selectivity. The endogenous concentration of Gal-3 in human plasma from HF patients was determined with results in agreement with those obtained using ELISA. The multiplexing feasibility of the developed immunoplatform was demonstrated for the simultaneous determination of Gal-3 and N-terminal pro-brain natriuretic peptide (NT-proBNP).
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138544
Piguillem Palacios, Sofía Viviana; María Gamella; García de Frutos, Pablo; Batlle, Montserrat; Yáñez Sedeño, Paloma; et al.; Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3; Wiley VCH Verlag; Electroanalysis; 32; 12; 8-2020; 2775-2785
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138544
identifier_str_mv Piguillem Palacios, Sofía Viviana; María Gamella; García de Frutos, Pablo; Batlle, Montserrat; Yáñez Sedeño, Paloma; et al.; Easily Multiplexable Immunoplatform to Assist Heart Failure Diagnosis through Amperometric Determination of Galectin-3; Wiley VCH Verlag; Electroanalysis; 32; 12; 8-2020; 2775-2785
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elan.202060323
info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.202060323
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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