High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat

Autores
Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; Pillonel, Amandine; Oszako, Tomasz; Belbahri, Lassâad
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups.
Fil: Singer, David. University of Neuchâte; Suiza
Fil: Lara, Enrique. University of Neuchâte; Suiza
Fil: Steciow, Mónica Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Seppey, Christophe V.W.. University of Neuchâte; Suiza
Fil: Paredes, Noelia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Pillonel, Amandine. University of Neuchâte; Suiza
Fil: Oszako, Tomasz. Forest Research Institute; Polonia
Fil: Belbahri, Lassâad. University of Neuchâte; Suiza
Materia
Diversity
Illumina Sequencing
Metabarcoding
Oomycetes
V9 Region Of the Ssu Rdna
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54327
Acceder
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network_name_str CONICET Digital (CONICET)
spelling High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitatSinger, DavidLara, EnriqueSteciow, Mónica MirtaSeppey, Christophe V.W.Paredes, Noelia IvanaPillonel, AmandineOszako, TomaszBelbahri, LassâadDiversityIllumina SequencingMetabarcodingOomycetesV9 Region Of the Ssu Rdnahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups.Fil: Singer, David. University of Neuchâte; SuizaFil: Lara, Enrique. University of Neuchâte; SuizaFil: Steciow, Mónica Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Seppey, Christophe V.W.. University of Neuchâte; SuizaFil: Paredes, Noelia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Pillonel, Amandine. University of Neuchâte; SuizaFil: Oszako, Tomasz. Forest Research Institute; PoloniaFil: Belbahri, Lassâad. University of Neuchâte; SuizaElsevier2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54327Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; et al.; High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat; Elsevier; Fungal Ecology; 23; 10-2016; 42-471754-5048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.funeco.2016.05.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1754504816300526info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:22Zoai:ri.conicet.gov.ar:11336/54327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:23.227CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
title High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
spellingShingle High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
Singer, David
Diversity
Illumina Sequencing
Metabarcoding
Oomycetes
V9 Region Of the Ssu Rdna
title_short High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
title_full High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
title_fullStr High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
title_full_unstemmed High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
title_sort High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
dc.creator.none.fl_str_mv Singer, David
Lara, Enrique
Steciow, Mónica Mirta
Seppey, Christophe V.W.
Paredes, Noelia Ivana
Pillonel, Amandine
Oszako, Tomasz
Belbahri, Lassâad
author Singer, David
author_facet Singer, David
Lara, Enrique
Steciow, Mónica Mirta
Seppey, Christophe V.W.
Paredes, Noelia Ivana
Pillonel, Amandine
Oszako, Tomasz
Belbahri, Lassâad
author_role author
author2 Lara, Enrique
Steciow, Mónica Mirta
Seppey, Christophe V.W.
Paredes, Noelia Ivana
Pillonel, Amandine
Oszako, Tomasz
Belbahri, Lassâad
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Diversity
Illumina Sequencing
Metabarcoding
Oomycetes
V9 Region Of the Ssu Rdna
topic Diversity
Illumina Sequencing
Metabarcoding
Oomycetes
V9 Region Of the Ssu Rdna
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups.
Fil: Singer, David. University of Neuchâte; Suiza
Fil: Lara, Enrique. University of Neuchâte; Suiza
Fil: Steciow, Mónica Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Seppey, Christophe V.W.. University of Neuchâte; Suiza
Fil: Paredes, Noelia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Pillonel, Amandine. University of Neuchâte; Suiza
Fil: Oszako, Tomasz. Forest Research Institute; Polonia
Fil: Belbahri, Lassâad. University of Neuchâte; Suiza
description Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54327
Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; et al.; High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat; Elsevier; Fungal Ecology; 23; 10-2016; 42-47
1754-5048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54327
identifier_str_mv Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; et al.; High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat; Elsevier; Fungal Ecology; 23; 10-2016; 42-47
1754-5048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.funeco.2016.05.009
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1754504816300526
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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