High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat
- Autores
- Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; Pillonel, Amandine; Oszako, Tomasz; Belbahri, Lassâad
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups.
Fil: Singer, David. University of Neuchâte; Suiza
Fil: Lara, Enrique. University of Neuchâte; Suiza
Fil: Steciow, Mónica Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Seppey, Christophe V.W.. University of Neuchâte; Suiza
Fil: Paredes, Noelia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Pillonel, Amandine. University of Neuchâte; Suiza
Fil: Oszako, Tomasz. Forest Research Institute; Polonia
Fil: Belbahri, Lassâad. University of Neuchâte; Suiza - Materia
-
Diversity
Illumina Sequencing
Metabarcoding
Oomycetes
V9 Region Of the Ssu Rdna - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54327
Ver los metadatos del registro completo
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High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitatSinger, DavidLara, EnriqueSteciow, Mónica MirtaSeppey, Christophe V.W.Paredes, Noelia IvanaPillonel, AmandineOszako, TomaszBelbahri, LassâadDiversityIllumina SequencingMetabarcodingOomycetesV9 Region Of the Ssu Rdnahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups.Fil: Singer, David. University of Neuchâte; SuizaFil: Lara, Enrique. University of Neuchâte; SuizaFil: Steciow, Mónica Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Seppey, Christophe V.W.. University of Neuchâte; SuizaFil: Paredes, Noelia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Pillonel, Amandine. University of Neuchâte; SuizaFil: Oszako, Tomasz. Forest Research Institute; PoloniaFil: Belbahri, Lassâad. University of Neuchâte; SuizaElsevier2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54327Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; et al.; High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat; Elsevier; Fungal Ecology; 23; 10-2016; 42-471754-5048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.funeco.2016.05.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1754504816300526info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:45Zoai:ri.conicet.gov.ar:11336/54327instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:45.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
title |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
spellingShingle |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat Singer, David Diversity Illumina Sequencing Metabarcoding Oomycetes V9 Region Of the Ssu Rdna |
title_short |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
title_full |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
title_fullStr |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
title_full_unstemmed |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
title_sort |
High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat |
dc.creator.none.fl_str_mv |
Singer, David Lara, Enrique Steciow, Mónica Mirta Seppey, Christophe V.W. Paredes, Noelia Ivana Pillonel, Amandine Oszako, Tomasz Belbahri, Lassâad |
author |
Singer, David |
author_facet |
Singer, David Lara, Enrique Steciow, Mónica Mirta Seppey, Christophe V.W. Paredes, Noelia Ivana Pillonel, Amandine Oszako, Tomasz Belbahri, Lassâad |
author_role |
author |
author2 |
Lara, Enrique Steciow, Mónica Mirta Seppey, Christophe V.W. Paredes, Noelia Ivana Pillonel, Amandine Oszako, Tomasz Belbahri, Lassâad |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Diversity Illumina Sequencing Metabarcoding Oomycetes V9 Region Of the Ssu Rdna |
topic |
Diversity Illumina Sequencing Metabarcoding Oomycetes V9 Region Of the Ssu Rdna |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups. Fil: Singer, David. University of Neuchâte; Suiza Fil: Lara, Enrique. University of Neuchâte; Suiza Fil: Steciow, Mónica Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina Fil: Seppey, Christophe V.W.. University of Neuchâte; Suiza Fil: Paredes, Noelia Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina Fil: Pillonel, Amandine. University of Neuchâte; Suiza Fil: Oszako, Tomasz. Forest Research Institute; Polonia Fil: Belbahri, Lassâad. University of Neuchâte; Suiza |
description |
Oomycete diversity has been generally underestimated, despite their ecological and economic importance. Surveying unexplored natural ecosystems with up-to-date molecular diversity tools can reveal the existence of unsuspected organisms. Here, we have explored the molecular diversity of five microhabitats located in five different oligotrophic peat bogs in the Jura Mountains using a high-throughput sequencing approach (Illumina HiSeq 2500). We found a total of 34 different phylotypes distributed in all major oomycete clades, and comprising sequences affiliated to both well-known phylotypes and members of undescribed, basal clades. Parasitic species, including obligate forms were well-represented, and phylotypes related to highly damaging invasive pathogens (Aphanomyces astaci: X1100 and Saprolegnia parasitica: X1602) were retrieved. Microhabitats differed significantly in their community composition, and many phylotypes were strongly affiliated to free water habitats (pools). Our approach proved effective in screening oomycete diversity in the studied habitat, and could be applied systematically to other environments and other fungal and fungal-like groups. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54327 Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; et al.; High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat; Elsevier; Fungal Ecology; 23; 10-2016; 42-47 1754-5048 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54327 |
identifier_str_mv |
Singer, David; Lara, Enrique; Steciow, Mónica Mirta; Seppey, Christophe V.W.; Paredes, Noelia Ivana; et al.; High-throughput sequencing reveals diverse oomycete communities in oligotrophic peat bog micro-habitat; Elsevier; Fungal Ecology; 23; 10-2016; 42-47 1754-5048 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.funeco.2016.05.009 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1754504816300526 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |