Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation

Autores
Mugnaini, Matías; Piatti, Veronica del Carmen; Schinder, Alejandro Fabián; Kropff, Emilio
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Hippocampal granule cells (GCs) are among the few neurons that are born throughout mammalian lifespan. It has been shown that young adult born GCs (4 weeks old) are transiently hyperplastic and excitable compared to mature ones (8 weeks old). While their inputs are well characterized, only a few studies address the maturation of GCs outputs. Here we aim to investigate the influence of developing adult born GCs on CA3, its main target. We hypothesize that evoked activity in CA3 reflects transient properties of young GCs. To explore this possibility, we performed optical stimulation of a cohort of adult born GCs expressing channelrhodopsin-2 in awake behaving mice while recording neuronal activity in CA3. We used different frequencies of stimulation at variable laser intensities to stimulate young and mature GCs. We found that mature GCs recruit more CA3 single unit activity, with frequency dependent facilitation. Evoked local field potentials followed a similar pattern. Interestingly, a small subset of putative pyramidal CA3 cells presented significantly high spiking levels as long as 50 ms after the light pulse. Only mature GCs were able to evoke this sustained activity. Is this persistent excitability caused by attractor dynamics? Do adult born neurons reshape de architecture of recurrent CA3 networks? These results open new challenges regarding the function of adult hippocampal neurogenesis and mnemonic networks dynamics dependent on the neurogenic niche.
Fil: Mugnaini, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Kropff, Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
2019 Meeting of Argentine Society for Research in Neurosciences
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
CA3
plasticity
opto-electrofisiology
neurogenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154628
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturationMugnaini, MatíasPiatti, Veronica del CarmenSchinder, Alejandro FabiánKropff, EmilioCA3plasticityopto-electrofisiologyneurogenesishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Hippocampal granule cells (GCs) are among the few neurons that are born throughout mammalian lifespan. It has been shown that young adult born GCs (4 weeks old) are transiently hyperplastic and excitable compared to mature ones (8 weeks old). While their inputs are well characterized, only a few studies address the maturation of GCs outputs. Here we aim to investigate the influence of developing adult born GCs on CA3, its main target. We hypothesize that evoked activity in CA3 reflects transient properties of young GCs. To explore this possibility, we performed optical stimulation of a cohort of adult born GCs expressing channelrhodopsin-2 in awake behaving mice while recording neuronal activity in CA3. We used different frequencies of stimulation at variable laser intensities to stimulate young and mature GCs. We found that mature GCs recruit more CA3 single unit activity, with frequency dependent facilitation. Evoked local field potentials followed a similar pattern. Interestingly, a small subset of putative pyramidal CA3 cells presented significantly high spiking levels as long as 50 ms after the light pulse. Only mature GCs were able to evoke this sustained activity. Is this persistent excitability caused by attractor dynamics? Do adult born neurons reshape de architecture of recurrent CA3 networks? These results open new challenges regarding the function of adult hippocampal neurogenesis and mnemonic networks dynamics dependent on the neurogenic niche.Fil: Mugnaini, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Kropff, Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina2019 Meeting of Argentine Society for Research in NeurosciencesVilla Carlos PazArgentinaSociedad Argentina de Investigación en NeurocienciasSAGE Publications2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/154628Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation; 2019 Meeting of Argentine Society for Research in Neurosciences; Villa Carlos Paz; Argentina; 2019; 112-1131759-0914CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1759091420979851Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:41:15Zoai:ri.conicet.gov.ar:11336/154628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:41:15.512CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
title Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
spellingShingle Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
Mugnaini, Matías
CA3
plasticity
opto-electrofisiology
neurogenesis
title_short Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
title_full Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
title_fullStr Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
title_full_unstemmed Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
title_sort Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation
dc.creator.none.fl_str_mv Mugnaini, Matías
Piatti, Veronica del Carmen
Schinder, Alejandro Fabián
Kropff, Emilio
author Mugnaini, Matías
author_facet Mugnaini, Matías
Piatti, Veronica del Carmen
Schinder, Alejandro Fabián
Kropff, Emilio
author_role author
author2 Piatti, Veronica del Carmen
Schinder, Alejandro Fabián
Kropff, Emilio
author2_role author
author
author
dc.subject.none.fl_str_mv CA3
plasticity
opto-electrofisiology
neurogenesis
topic CA3
plasticity
opto-electrofisiology
neurogenesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hippocampal granule cells (GCs) are among the few neurons that are born throughout mammalian lifespan. It has been shown that young adult born GCs (4 weeks old) are transiently hyperplastic and excitable compared to mature ones (8 weeks old). While their inputs are well characterized, only a few studies address the maturation of GCs outputs. Here we aim to investigate the influence of developing adult born GCs on CA3, its main target. We hypothesize that evoked activity in CA3 reflects transient properties of young GCs. To explore this possibility, we performed optical stimulation of a cohort of adult born GCs expressing channelrhodopsin-2 in awake behaving mice while recording neuronal activity in CA3. We used different frequencies of stimulation at variable laser intensities to stimulate young and mature GCs. We found that mature GCs recruit more CA3 single unit activity, with frequency dependent facilitation. Evoked local field potentials followed a similar pattern. Interestingly, a small subset of putative pyramidal CA3 cells presented significantly high spiking levels as long as 50 ms after the light pulse. Only mature GCs were able to evoke this sustained activity. Is this persistent excitability caused by attractor dynamics? Do adult born neurons reshape de architecture of recurrent CA3 networks? These results open new challenges regarding the function of adult hippocampal neurogenesis and mnemonic networks dynamics dependent on the neurogenic niche.
Fil: Mugnaini, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Piatti, Veronica del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Kropff, Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
2019 Meeting of Argentine Society for Research in Neurosciences
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Hippocampal granule cells (GCs) are among the few neurons that are born throughout mammalian lifespan. It has been shown that young adult born GCs (4 weeks old) are transiently hyperplastic and excitable compared to mature ones (8 weeks old). While their inputs are well characterized, only a few studies address the maturation of GCs outputs. Here we aim to investigate the influence of developing adult born GCs on CA3, its main target. We hypothesize that evoked activity in CA3 reflects transient properties of young GCs. To explore this possibility, we performed optical stimulation of a cohort of adult born GCs expressing channelrhodopsin-2 in awake behaving mice while recording neuronal activity in CA3. We used different frequencies of stimulation at variable laser intensities to stimulate young and mature GCs. We found that mature GCs recruit more CA3 single unit activity, with frequency dependent facilitation. Evoked local field potentials followed a similar pattern. Interestingly, a small subset of putative pyramidal CA3 cells presented significantly high spiking levels as long as 50 ms after the light pulse. Only mature GCs were able to evoke this sustained activity. Is this persistent excitability caused by attractor dynamics? Do adult born neurons reshape de architecture of recurrent CA3 networks? These results open new challenges regarding the function of adult hippocampal neurogenesis and mnemonic networks dynamics dependent on the neurogenic niche.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154628
Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation; 2019 Meeting of Argentine Society for Research in Neurosciences; Villa Carlos Paz; Argentina; 2019; 112-113
1759-0914
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154628
identifier_str_mv Adult born dentate granule cells evoke CA3 activity with a gain that increases along maturation; 2019 Meeting of Argentine Society for Research in Neurosciences; Villa Carlos Paz; Argentina; 2019; 112-113
1759-0914
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1759091420979851
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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