Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy

Autores
García Parra, Miguel Angel; Roa Acosta, Diego Fernando; García Londoño, Víctor Alonso; Moreno Medina, Brigitte; Bravo Gomez, Jesús
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antioxidant capacities from six quinoa cultivars with high productive potential from central regions in Colombia. This study used middle-range infrared spectroscopy (IR-MIR) to determine the proteins, starch and lipids distinctive to quinoa grains. Ultra-high-performance liquid chromatography electrospray ionization Orbitrap, along with high-resolution mass spectrometry (UHPLC/ESI-Orbitrap MS), were also used to identify the existence of polyphenols in cultivars. The antioxidant capacity was determined through DPPH, ABTS and FRAP. The spectrums exhibited significant variances on the transmittance bands associated with 2922 cm−1, 1016 cm−1 and 1633 cm−1. Moreover, the intensity variations on the peaks from the secondary protein structure were identified, mainly on the bands associated with β-Sheet-1 and-2, random coil α elice and β-turns-2 and-3. Changes found in the ratios 996 cm−1/1014 cm−1 and 1041 cm−1/1014 cm−1 were associated with the crys-talline/amorphous affinity. Regarding the antioxidant capacity, great differences were identified (p < 0.001) mainly through FRAP methods, while the phenolic acids and flavonoids were determined by UHPLC/ESI-Orbitrap MS techniques. The presence of apigenin and pinocembrin on grains was reported for the first time. Titicaca and Nariño were the most phytochemically diverse quinoa seeds.
Fil: García Parra, Miguel Angel. Universidad del Cauca; Colombia
Fil: Roa Acosta, Diego Fernando. Universidad del Cauca; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Londoño, Víctor Alonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnología en Polímeros y Nanotecnología. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnología en Polímeros y Nanotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Moreno Medina, Brigitte. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Bravo Gomez, Jesús. Universidad del Cauca; Colombia
Materia
AMARANTHACEAE
CHROMATOGRAPHY
POLYPHENOL
QUINOA STARCH
SECONDARY PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181215
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181215
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS SpectroscopyGarcía Parra, Miguel AngelRoa Acosta, Diego FernandoGarcía Londoño, Víctor AlonsoMoreno Medina, BrigitteBravo Gomez, JesúsAMARANTHACEAECHROMATOGRAPHYPOLYPHENOLQUINOA STARCHSECONDARY PROTEINhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antioxidant capacities from six quinoa cultivars with high productive potential from central regions in Colombia. This study used middle-range infrared spectroscopy (IR-MIR) to determine the proteins, starch and lipids distinctive to quinoa grains. Ultra-high-performance liquid chromatography electrospray ionization Orbitrap, along with high-resolution mass spectrometry (UHPLC/ESI-Orbitrap MS), were also used to identify the existence of polyphenols in cultivars. The antioxidant capacity was determined through DPPH, ABTS and FRAP. The spectrums exhibited significant variances on the transmittance bands associated with 2922 cm−1, 1016 cm−1 and 1633 cm−1. Moreover, the intensity variations on the peaks from the secondary protein structure were identified, mainly on the bands associated with β-Sheet-1 and-2, random coil α elice and β-turns-2 and-3. Changes found in the ratios 996 cm−1/1014 cm−1 and 1041 cm−1/1014 cm−1 were associated with the crys-talline/amorphous affinity. Regarding the antioxidant capacity, great differences were identified (p < 0.001) mainly through FRAP methods, while the phenolic acids and flavonoids were determined by UHPLC/ESI-Orbitrap MS techniques. The presence of apigenin and pinocembrin on grains was reported for the first time. Titicaca and Nariño were the most phytochemically diverse quinoa seeds.Fil: García Parra, Miguel Angel. Universidad del Cauca; ColombiaFil: Roa Acosta, Diego Fernando. Universidad del Cauca; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García Londoño, Víctor Alonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnología en Polímeros y Nanotecnología. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnología en Polímeros y Nanotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Moreno Medina, Brigitte. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Bravo Gomez, Jesús. Universidad del Cauca; ColombiaMDPI2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181215García Parra, Miguel Angel; Roa Acosta, Diego Fernando; García Londoño, Víctor Alonso; Moreno Medina, Brigitte; Bravo Gomez, Jesús; Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy; MDPI; Plants; 10; 10; 10-2021; 1-152223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/10/2159info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10102159info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:09Zoai:ri.conicet.gov.ar:11336/181215instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:09.23CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
title Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
spellingShingle Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
García Parra, Miguel Angel
AMARANTHACEAE
CHROMATOGRAPHY
POLYPHENOL
QUINOA STARCH
SECONDARY PROTEIN
title_short Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
title_full Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
title_fullStr Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
title_full_unstemmed Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
title_sort Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
dc.creator.none.fl_str_mv García Parra, Miguel Angel
Roa Acosta, Diego Fernando
García Londoño, Víctor Alonso
Moreno Medina, Brigitte
Bravo Gomez, Jesús
author García Parra, Miguel Angel
author_facet García Parra, Miguel Angel
Roa Acosta, Diego Fernando
García Londoño, Víctor Alonso
Moreno Medina, Brigitte
Bravo Gomez, Jesús
author_role author
author2 Roa Acosta, Diego Fernando
García Londoño, Víctor Alonso
Moreno Medina, Brigitte
Bravo Gomez, Jesús
author2_role author
author
author
author
dc.subject.none.fl_str_mv AMARANTHACEAE
CHROMATOGRAPHY
POLYPHENOL
QUINOA STARCH
SECONDARY PROTEIN
topic AMARANTHACEAE
CHROMATOGRAPHY
POLYPHENOL
QUINOA STARCH
SECONDARY PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antioxidant capacities from six quinoa cultivars with high productive potential from central regions in Colombia. This study used middle-range infrared spectroscopy (IR-MIR) to determine the proteins, starch and lipids distinctive to quinoa grains. Ultra-high-performance liquid chromatography electrospray ionization Orbitrap, along with high-resolution mass spectrometry (UHPLC/ESI-Orbitrap MS), were also used to identify the existence of polyphenols in cultivars. The antioxidant capacity was determined through DPPH, ABTS and FRAP. The spectrums exhibited significant variances on the transmittance bands associated with 2922 cm−1, 1016 cm−1 and 1633 cm−1. Moreover, the intensity variations on the peaks from the secondary protein structure were identified, mainly on the bands associated with β-Sheet-1 and-2, random coil α elice and β-turns-2 and-3. Changes found in the ratios 996 cm−1/1014 cm−1 and 1041 cm−1/1014 cm−1 were associated with the crys-talline/amorphous affinity. Regarding the antioxidant capacity, great differences were identified (p < 0.001) mainly through FRAP methods, while the phenolic acids and flavonoids were determined by UHPLC/ESI-Orbitrap MS techniques. The presence of apigenin and pinocembrin on grains was reported for the first time. Titicaca and Nariño were the most phytochemically diverse quinoa seeds.
Fil: García Parra, Miguel Angel. Universidad del Cauca; Colombia
Fil: Roa Acosta, Diego Fernando. Universidad del Cauca; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Londoño, Víctor Alonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnología en Polímeros y Nanotecnología. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnología en Polímeros y Nanotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Moreno Medina, Brigitte. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Bravo Gomez, Jesús. Universidad del Cauca; Colombia
description The existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antioxidant capacities from six quinoa cultivars with high productive potential from central regions in Colombia. This study used middle-range infrared spectroscopy (IR-MIR) to determine the proteins, starch and lipids distinctive to quinoa grains. Ultra-high-performance liquid chromatography electrospray ionization Orbitrap, along with high-resolution mass spectrometry (UHPLC/ESI-Orbitrap MS), were also used to identify the existence of polyphenols in cultivars. The antioxidant capacity was determined through DPPH, ABTS and FRAP. The spectrums exhibited significant variances on the transmittance bands associated with 2922 cm−1, 1016 cm−1 and 1633 cm−1. Moreover, the intensity variations on the peaks from the secondary protein structure were identified, mainly on the bands associated with β-Sheet-1 and-2, random coil α elice and β-turns-2 and-3. Changes found in the ratios 996 cm−1/1014 cm−1 and 1041 cm−1/1014 cm−1 were associated with the crys-talline/amorphous affinity. Regarding the antioxidant capacity, great differences were identified (p < 0.001) mainly through FRAP methods, while the phenolic acids and flavonoids were determined by UHPLC/ESI-Orbitrap MS techniques. The presence of apigenin and pinocembrin on grains was reported for the first time. Titicaca and Nariño were the most phytochemically diverse quinoa seeds.
publishDate 2021
dc.date.none.fl_str_mv 2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181215
García Parra, Miguel Angel; Roa Acosta, Diego Fernando; García Londoño, Víctor Alonso; Moreno Medina, Brigitte; Bravo Gomez, Jesús; Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy; MDPI; Plants; 10; 10; 10-2021; 1-15
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181215
identifier_str_mv García Parra, Miguel Angel; Roa Acosta, Diego Fernando; García Londoño, Víctor Alonso; Moreno Medina, Brigitte; Bravo Gomez, Jesús; Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy; MDPI; Plants; 10; 10; 10-2021; 1-15
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/10/2159
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10102159
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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