Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3

Autores
Escobar, Mariana; Herrera Seitz, Karina; Studdert, Claudia Alicia
Año de publicación
2018
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Halomonas titanicae KHS3 was isolated from hydrocarbon-contaminated water in Mar del Plata harbor. This strain is able to accumulate polyhydroxyalkanoates (PHAs), reserve polymers that can be used as raw material for the preparation of bioplastics.The aim of this work was to evaluate the ability of H. titanicae KHS3 to use glycerol as the only source of carbon and energy and convert it into PHAs.When grown in mineral salts medium with 0.25% commercial glycerol, PHAs synthesis was only moderate. However, when cells were harvested at mid-exponential phase and resuspended in medium depleted of nitrogen source, PHA accumulation was dramatically increased and reached up to 70% of dry cell weight. Such accumulation also occurred when cultures were fed with glycerol obtained from a biodiesel reactor, both in its crude form (contaminated with methanol and salts) and partially purified (technical grade), suggesting that this bioconversion potentially represents a way of adding value to the otherwise disposable glycerol.We show the kinetics of PHA accumulation after nitrogen deprivation under different conditions. Good PHA accumulation occurred in media containing between 2 and 10% NaCl. PHAs inside cells remained stable after long incubations in nitrogen-lacking medium, but decreased rapidly after re-addition of ammonium sulfate.The purified polymer is currently analyzed by RMN to determine its precise chemical composition.
Fil: Escobar, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Entre Ríos
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
Halomonas
PHAs
glicerol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155579
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/155579
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3Escobar, MarianaHerrera Seitz, KarinaStuddert, Claudia AliciaHalomonasPHAsglicerolhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Halomonas titanicae KHS3 was isolated from hydrocarbon-contaminated water in Mar del Plata harbor. This strain is able to accumulate polyhydroxyalkanoates (PHAs), reserve polymers that can be used as raw material for the preparation of bioplastics.The aim of this work was to evaluate the ability of H. titanicae KHS3 to use glycerol as the only source of carbon and energy and convert it into PHAs.When grown in mineral salts medium with 0.25% commercial glycerol, PHAs synthesis was only moderate. However, when cells were harvested at mid-exponential phase and resuspended in medium depleted of nitrogen source, PHA accumulation was dramatically increased and reached up to 70% of dry cell weight. Such accumulation also occurred when cultures were fed with glycerol obtained from a biodiesel reactor, both in its crude form (contaminated with methanol and salts) and partially purified (technical grade), suggesting that this bioconversion potentially represents a way of adding value to the otherwise disposable glycerol.We show the kinetics of PHA accumulation after nitrogen deprivation under different conditions. Good PHA accumulation occurred in media containing between 2 and 10% NaCl. PHAs inside cells remained stable after long incubations in nitrogen-lacking medium, but decreased rapidly after re-addition of ammonium sulfate.The purified polymer is currently analyzed by RMN to determine its precise chemical composition.Fil: Escobar, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina54th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyEntre RíosArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularBiocell2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155579Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Entre Ríos; Argentina; 2018; 1-81667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:31Zoai:ri.conicet.gov.ar:11336/155579instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:31.718CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
title Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
spellingShingle Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
Escobar, Mariana
Halomonas
PHAs
glicerol
title_short Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
title_full Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
title_fullStr Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
title_full_unstemmed Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
title_sort Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3
dc.creator.none.fl_str_mv Escobar, Mariana
Herrera Seitz, Karina
Studdert, Claudia Alicia
author Escobar, Mariana
author_facet Escobar, Mariana
Herrera Seitz, Karina
Studdert, Claudia Alicia
author_role author
author2 Herrera Seitz, Karina
Studdert, Claudia Alicia
author2_role author
author
dc.subject.none.fl_str_mv Halomonas
PHAs
glicerol
topic Halomonas
PHAs
glicerol
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Halomonas titanicae KHS3 was isolated from hydrocarbon-contaminated water in Mar del Plata harbor. This strain is able to accumulate polyhydroxyalkanoates (PHAs), reserve polymers that can be used as raw material for the preparation of bioplastics.The aim of this work was to evaluate the ability of H. titanicae KHS3 to use glycerol as the only source of carbon and energy and convert it into PHAs.When grown in mineral salts medium with 0.25% commercial glycerol, PHAs synthesis was only moderate. However, when cells were harvested at mid-exponential phase and resuspended in medium depleted of nitrogen source, PHA accumulation was dramatically increased and reached up to 70% of dry cell weight. Such accumulation also occurred when cultures were fed with glycerol obtained from a biodiesel reactor, both in its crude form (contaminated with methanol and salts) and partially purified (technical grade), suggesting that this bioconversion potentially represents a way of adding value to the otherwise disposable glycerol.We show the kinetics of PHA accumulation after nitrogen deprivation under different conditions. Good PHA accumulation occurred in media containing between 2 and 10% NaCl. PHAs inside cells remained stable after long incubations in nitrogen-lacking medium, but decreased rapidly after re-addition of ammonium sulfate.The purified polymer is currently analyzed by RMN to determine its precise chemical composition.
Fil: Escobar, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Entre Ríos
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Halomonas titanicae KHS3 was isolated from hydrocarbon-contaminated water in Mar del Plata harbor. This strain is able to accumulate polyhydroxyalkanoates (PHAs), reserve polymers that can be used as raw material for the preparation of bioplastics.The aim of this work was to evaluate the ability of H. titanicae KHS3 to use glycerol as the only source of carbon and energy and convert it into PHAs.When grown in mineral salts medium with 0.25% commercial glycerol, PHAs synthesis was only moderate. However, when cells were harvested at mid-exponential phase and resuspended in medium depleted of nitrogen source, PHA accumulation was dramatically increased and reached up to 70% of dry cell weight. Such accumulation also occurred when cultures were fed with glycerol obtained from a biodiesel reactor, both in its crude form (contaminated with methanol and salts) and partially purified (technical grade), suggesting that this bioconversion potentially represents a way of adding value to the otherwise disposable glycerol.We show the kinetics of PHA accumulation after nitrogen deprivation under different conditions. Good PHA accumulation occurred in media containing between 2 and 10% NaCl. PHAs inside cells remained stable after long incubations in nitrogen-lacking medium, but decreased rapidly after re-addition of ammonium sulfate.The purified polymer is currently analyzed by RMN to determine its precise chemical composition.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155579
Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Entre Ríos; Argentina; 2018; 1-8
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155579
identifier_str_mv Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Entre Ríos; Argentina; 2018; 1-8
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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