Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho

Autores
Larraburu, Ezequiel Enrique; Yarte, Mauro Enrique; Llorente, Berta Elizabet
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Optimal in vitro plant growth can be stimulated by selecting specific nutritional and environmental conditions. However, the culture conditions, dissection, and disinfection of plant material are stressful and may induce disruption of the plant physiological homeostasis. This can be modified by inoculation with rhizobacteria as Azospirillum brasilense, by the culture medium type, and by auxin induction. Here, we performed rooting experiments in two auxin-free culture media with ‘pink lapacho’ (Handroanthus impetiginosus) shoots previously induced with 0, 10, 30, or 50 μM indole butyric acid (IBA) for 3 days and inoculated with A. brasilense Cd and Az39. Peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) activities were determined on days 0, 3, 6, 9, 12, and 15. Also, weekly absolute rooting percentage was evaluated. All enzymatic activities were higher in A. brasilense-inoculated shoots, linked to early and high rooting percentage. The culture medium type and IBA concentration also affected enzymatic activities. The positive correlation between PO and PAL activities on day 9 and successful final in vitro rooting of H. impetiginosus allows using these activities as early markers of rhizogenesis reducing the selection time of easy-to-root plants. The changes in enzymatic levels performed here are discussed on the basis of their role in rooting and in vitro stress and contribute to the knowledge of the physiology of trees and their interaction with rhizobacteria.
Fil: Larraburu, Ezequiel Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Yarte, Mauro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Llorente, Berta Elizabet. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Materia
HANDROANTHUS IMPETIGINOSUS
IN VITRO ROOTING
PEROXIDASE
PHENYLALANINE AMMONIA-LYASE
POLYPHENOL OXIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179040
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/179040
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapachoLarraburu, Ezequiel EnriqueYarte, Mauro EnriqueLlorente, Berta ElizabetHANDROANTHUS IMPETIGINOSUSIN VITRO ROOTINGPEROXIDASEPHENYLALANINE AMMONIA-LYASEPOLYPHENOL OXIDASEhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Optimal in vitro plant growth can be stimulated by selecting specific nutritional and environmental conditions. However, the culture conditions, dissection, and disinfection of plant material are stressful and may induce disruption of the plant physiological homeostasis. This can be modified by inoculation with rhizobacteria as Azospirillum brasilense, by the culture medium type, and by auxin induction. Here, we performed rooting experiments in two auxin-free culture media with ‘pink lapacho’ (Handroanthus impetiginosus) shoots previously induced with 0, 10, 30, or 50 μM indole butyric acid (IBA) for 3 days and inoculated with A. brasilense Cd and Az39. Peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) activities were determined on days 0, 3, 6, 9, 12, and 15. Also, weekly absolute rooting percentage was evaluated. All enzymatic activities were higher in A. brasilense-inoculated shoots, linked to early and high rooting percentage. The culture medium type and IBA concentration also affected enzymatic activities. The positive correlation between PO and PAL activities on day 9 and successful final in vitro rooting of H. impetiginosus allows using these activities as early markers of rhizogenesis reducing the selection time of easy-to-root plants. The changes in enzymatic levels performed here are discussed on the basis of their role in rooting and in vitro stress and contribute to the knowledge of the physiology of trees and their interaction with rhizobacteria.Fil: Larraburu, Ezequiel Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaFil: Yarte, Mauro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaFil: Llorente, Berta Elizabet. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaSpringer2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179040Larraburu, Ezequiel Enrique; Yarte, Mauro Enrique; Llorente, Berta Elizabet; Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho; Springer; Plant Cell, Tissue and Organ Culture; 127; 2; 8-2016; 381-3920167-6857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-016-1060-zinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-016-1060-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:36Zoai:ri.conicet.gov.ar:11336/179040instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:36.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
title Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
spellingShingle Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
Larraburu, Ezequiel Enrique
HANDROANTHUS IMPETIGINOSUS
IN VITRO ROOTING
PEROXIDASE
PHENYLALANINE AMMONIA-LYASE
POLYPHENOL OXIDASE
title_short Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
title_full Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
title_fullStr Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
title_full_unstemmed Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
title_sort Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho
dc.creator.none.fl_str_mv Larraburu, Ezequiel Enrique
Yarte, Mauro Enrique
Llorente, Berta Elizabet
author Larraburu, Ezequiel Enrique
author_facet Larraburu, Ezequiel Enrique
Yarte, Mauro Enrique
Llorente, Berta Elizabet
author_role author
author2 Yarte, Mauro Enrique
Llorente, Berta Elizabet
author2_role author
author
dc.subject.none.fl_str_mv HANDROANTHUS IMPETIGINOSUS
IN VITRO ROOTING
PEROXIDASE
PHENYLALANINE AMMONIA-LYASE
POLYPHENOL OXIDASE
topic HANDROANTHUS IMPETIGINOSUS
IN VITRO ROOTING
PEROXIDASE
PHENYLALANINE AMMONIA-LYASE
POLYPHENOL OXIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Optimal in vitro plant growth can be stimulated by selecting specific nutritional and environmental conditions. However, the culture conditions, dissection, and disinfection of plant material are stressful and may induce disruption of the plant physiological homeostasis. This can be modified by inoculation with rhizobacteria as Azospirillum brasilense, by the culture medium type, and by auxin induction. Here, we performed rooting experiments in two auxin-free culture media with ‘pink lapacho’ (Handroanthus impetiginosus) shoots previously induced with 0, 10, 30, or 50 μM indole butyric acid (IBA) for 3 days and inoculated with A. brasilense Cd and Az39. Peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) activities were determined on days 0, 3, 6, 9, 12, and 15. Also, weekly absolute rooting percentage was evaluated. All enzymatic activities were higher in A. brasilense-inoculated shoots, linked to early and high rooting percentage. The culture medium type and IBA concentration also affected enzymatic activities. The positive correlation between PO and PAL activities on day 9 and successful final in vitro rooting of H. impetiginosus allows using these activities as early markers of rhizogenesis reducing the selection time of easy-to-root plants. The changes in enzymatic levels performed here are discussed on the basis of their role in rooting and in vitro stress and contribute to the knowledge of the physiology of trees and their interaction with rhizobacteria.
Fil: Larraburu, Ezequiel Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Yarte, Mauro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Llorente, Berta Elizabet. Universidad Nacional de Lujan. Departamento de Cs.basicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
description Optimal in vitro plant growth can be stimulated by selecting specific nutritional and environmental conditions. However, the culture conditions, dissection, and disinfection of plant material are stressful and may induce disruption of the plant physiological homeostasis. This can be modified by inoculation with rhizobacteria as Azospirillum brasilense, by the culture medium type, and by auxin induction. Here, we performed rooting experiments in two auxin-free culture media with ‘pink lapacho’ (Handroanthus impetiginosus) shoots previously induced with 0, 10, 30, or 50 μM indole butyric acid (IBA) for 3 days and inoculated with A. brasilense Cd and Az39. Peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) activities were determined on days 0, 3, 6, 9, 12, and 15. Also, weekly absolute rooting percentage was evaluated. All enzymatic activities were higher in A. brasilense-inoculated shoots, linked to early and high rooting percentage. The culture medium type and IBA concentration also affected enzymatic activities. The positive correlation between PO and PAL activities on day 9 and successful final in vitro rooting of H. impetiginosus allows using these activities as early markers of rhizogenesis reducing the selection time of easy-to-root plants. The changes in enzymatic levels performed here are discussed on the basis of their role in rooting and in vitro stress and contribute to the knowledge of the physiology of trees and their interaction with rhizobacteria.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179040
Larraburu, Ezequiel Enrique; Yarte, Mauro Enrique; Llorente, Berta Elizabet; Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho; Springer; Plant Cell, Tissue and Organ Culture; 127; 2; 8-2016; 381-392
0167-6857
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179040
identifier_str_mv Larraburu, Ezequiel Enrique; Yarte, Mauro Enrique; Llorente, Berta Elizabet; Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho; Springer; Plant Cell, Tissue and Organ Culture; 127; 2; 8-2016; 381-392
0167-6857
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11240-016-1060-z
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-016-1060-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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