A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules

Autores
Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Permingeat, Hugo Raúl; Souza Canada, Eduardo Daniel; Siena, Lorena Adelina; Spoto, Nicolás Leandro; Galdeano, Florencia; Espinoza, Francisco; Leblanc, Olivier; Pessino, Silvina Claudia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovulenucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINESYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing expression levels positively correlated with sexuality rates in facultative apomicticPaspalum notatum. PN_ TGS1-like displayed contrasting in situ hybridization patterns in apomictic and sexual plant ovules frompremeiosis to anthesis. Here we transformed sexual Paspalum notatum with a TGS1-like antisense construction under aconstitutive promoter, in order to produce lines with reduced transcript representation. Antisense plants developed prominenttrichomes on the adaxial leaf surface, a trait absent from control genotypes. Reproductive development analysis revealedoccasional formation of twin ovules. While control individuals typically displayed a single meiotic embryo sac per ovule, antisenselines showed 12.93-15.79% of ovules bearing extra nuclei, which can be assigned to aposporous-like embryo sacs (AES-like) or,alternatively, to gametophytes with a misguided cell fate development. Moreover, around 8.42-9.52% of ovules showed what lookedlike a combination of meiotic and aposporous-like sacs. Besides, 32.5% of ovules at early developmental stages displayed nucellarcells with prominent nuclei resembling apospory initials (AIs), which surrounded the megaspore mother cell (MMC) or theMMC-derived meiotic products. Two or more concurrent meiosis events were never detected, which suggest a non-reduced naturefor the extra nuclei observed in the mature ovules, unless they were generated by proliferation and misguided differentiation ofthe legitimate meiotic products. The antisense lines produced a similar amount of viable even-sized pollen with respect to controlgenotypes, and formed an equivalent full seed set (~9% of total seeds) after self-pollination. Flow cytometry analyses of caryopsesderived from antisense lines revealed that all full seeds had originated from meiotic embryo sacs (i.e. by sexuality). A reduction of25.55 % in the germination percentage was detected when comparing antisense lines with controls. Our results indicate that PN_TGS1-like influences ovule, gametophyte and possibly embryo development.
Fil: Colono, Carolina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Permingeat, Hugo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Souza Canada, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Siena, Lorena Adelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Spoto, Nicolás Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Leblanc, Olivier. No especifíca;
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
APOMIXIS
APOSPORY
METHYLTRANSFERASE
PLANT REPRODUCTION
TGS1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163480
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163480
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovulesColono, Carolina MartaOrtiz, Juan Pablo AmelioPermingeat, Hugo RaúlSouza Canada, Eduardo DanielSiena, Lorena AdelinaSpoto, Nicolás LeandroGaldeano, FlorenciaEspinoza, FranciscoLeblanc, OlivierPessino, Silvina ClaudiaAPOMIXISAPOSPORYMETHYLTRANSFERASEPLANT REPRODUCTIONTGS1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovulenucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINESYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing expression levels positively correlated with sexuality rates in facultative apomicticPaspalum notatum. PN_ TGS1-like displayed contrasting in situ hybridization patterns in apomictic and sexual plant ovules frompremeiosis to anthesis. Here we transformed sexual Paspalum notatum with a TGS1-like antisense construction under aconstitutive promoter, in order to produce lines with reduced transcript representation. Antisense plants developed prominenttrichomes on the adaxial leaf surface, a trait absent from control genotypes. Reproductive development analysis revealedoccasional formation of twin ovules. While control individuals typically displayed a single meiotic embryo sac per ovule, antisenselines showed 12.93-15.79% of ovules bearing extra nuclei, which can be assigned to aposporous-like embryo sacs (AES-like) or,alternatively, to gametophytes with a misguided cell fate development. Moreover, around 8.42-9.52% of ovules showed what lookedlike a combination of meiotic and aposporous-like sacs. Besides, 32.5% of ovules at early developmental stages displayed nucellarcells with prominent nuclei resembling apospory initials (AIs), which surrounded the megaspore mother cell (MMC) or theMMC-derived meiotic products. Two or more concurrent meiosis events were never detected, which suggest a non-reduced naturefor the extra nuclei observed in the mature ovules, unless they were generated by proliferation and misguided differentiation ofthe legitimate meiotic products. The antisense lines produced a similar amount of viable even-sized pollen with respect to controlgenotypes, and formed an equivalent full seed set (~9% of total seeds) after self-pollination. Flow cytometry analyses of caryopsesderived from antisense lines revealed that all full seeds had originated from meiotic embryo sacs (i.e. by sexuality). A reduction of25.55 % in the germination percentage was detected when comparing antisense lines with controls. Our results indicate that PN_TGS1-like influences ovule, gametophyte and possibly embryo development.Fil: Colono, Carolina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Permingeat, Hugo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Souza Canada, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Siena, Lorena Adelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Spoto, Nicolás Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Leblanc, Olivier. No especifíca;Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFrontiers Media2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163480Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Permingeat, Hugo Raúl; Souza Canada, Eduardo Daniel; Siena, Lorena Adelina; et al.; A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules; Frontiers Media; Frontiers in Plant Science; 10; 11-2019; 1-141664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2019.01566/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2019.01566info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:44Zoai:ri.conicet.gov.ar:11336/163480instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:45.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
spellingShingle A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
Colono, Carolina Marta
APOMIXIS
APOSPORY
METHYLTRANSFERASE
PLANT REPRODUCTION
TGS1
title_short A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_full A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_fullStr A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_full_unstemmed A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_sort A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
dc.creator.none.fl_str_mv Colono, Carolina Marta
Ortiz, Juan Pablo Amelio
Permingeat, Hugo Raúl
Souza Canada, Eduardo Daniel
Siena, Lorena Adelina
Spoto, Nicolás Leandro
Galdeano, Florencia
Espinoza, Francisco
Leblanc, Olivier
Pessino, Silvina Claudia
author Colono, Carolina Marta
author_facet Colono, Carolina Marta
Ortiz, Juan Pablo Amelio
Permingeat, Hugo Raúl
Souza Canada, Eduardo Daniel
Siena, Lorena Adelina
Spoto, Nicolás Leandro
Galdeano, Florencia
Espinoza, Francisco
Leblanc, Olivier
Pessino, Silvina Claudia
author_role author
author2 Ortiz, Juan Pablo Amelio
Permingeat, Hugo Raúl
Souza Canada, Eduardo Daniel
Siena, Lorena Adelina
Spoto, Nicolás Leandro
Galdeano, Florencia
Espinoza, Francisco
Leblanc, Olivier
Pessino, Silvina Claudia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOMIXIS
APOSPORY
METHYLTRANSFERASE
PLANT REPRODUCTION
TGS1
topic APOMIXIS
APOSPORY
METHYLTRANSFERASE
PLANT REPRODUCTION
TGS1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovulenucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINESYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing expression levels positively correlated with sexuality rates in facultative apomicticPaspalum notatum. PN_ TGS1-like displayed contrasting in situ hybridization patterns in apomictic and sexual plant ovules frompremeiosis to anthesis. Here we transformed sexual Paspalum notatum with a TGS1-like antisense construction under aconstitutive promoter, in order to produce lines with reduced transcript representation. Antisense plants developed prominenttrichomes on the adaxial leaf surface, a trait absent from control genotypes. Reproductive development analysis revealedoccasional formation of twin ovules. While control individuals typically displayed a single meiotic embryo sac per ovule, antisenselines showed 12.93-15.79% of ovules bearing extra nuclei, which can be assigned to aposporous-like embryo sacs (AES-like) or,alternatively, to gametophytes with a misguided cell fate development. Moreover, around 8.42-9.52% of ovules showed what lookedlike a combination of meiotic and aposporous-like sacs. Besides, 32.5% of ovules at early developmental stages displayed nucellarcells with prominent nuclei resembling apospory initials (AIs), which surrounded the megaspore mother cell (MMC) or theMMC-derived meiotic products. Two or more concurrent meiosis events were never detected, which suggest a non-reduced naturefor the extra nuclei observed in the mature ovules, unless they were generated by proliferation and misguided differentiation ofthe legitimate meiotic products. The antisense lines produced a similar amount of viable even-sized pollen with respect to controlgenotypes, and formed an equivalent full seed set (~9% of total seeds) after self-pollination. Flow cytometry analyses of caryopsesderived from antisense lines revealed that all full seeds had originated from meiotic embryo sacs (i.e. by sexuality). A reduction of25.55 % in the germination percentage was detected when comparing antisense lines with controls. Our results indicate that PN_TGS1-like influences ovule, gametophyte and possibly embryo development.
Fil: Colono, Carolina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Permingeat, Hugo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Souza Canada, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Siena, Lorena Adelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Spoto, Nicolás Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Leblanc, Olivier. No especifíca;
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovulenucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINESYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing expression levels positively correlated with sexuality rates in facultative apomicticPaspalum notatum. PN_ TGS1-like displayed contrasting in situ hybridization patterns in apomictic and sexual plant ovules frompremeiosis to anthesis. Here we transformed sexual Paspalum notatum with a TGS1-like antisense construction under aconstitutive promoter, in order to produce lines with reduced transcript representation. Antisense plants developed prominenttrichomes on the adaxial leaf surface, a trait absent from control genotypes. Reproductive development analysis revealedoccasional formation of twin ovules. While control individuals typically displayed a single meiotic embryo sac per ovule, antisenselines showed 12.93-15.79% of ovules bearing extra nuclei, which can be assigned to aposporous-like embryo sacs (AES-like) or,alternatively, to gametophytes with a misguided cell fate development. Moreover, around 8.42-9.52% of ovules showed what lookedlike a combination of meiotic and aposporous-like sacs. Besides, 32.5% of ovules at early developmental stages displayed nucellarcells with prominent nuclei resembling apospory initials (AIs), which surrounded the megaspore mother cell (MMC) or theMMC-derived meiotic products. Two or more concurrent meiosis events were never detected, which suggest a non-reduced naturefor the extra nuclei observed in the mature ovules, unless they were generated by proliferation and misguided differentiation ofthe legitimate meiotic products. The antisense lines produced a similar amount of viable even-sized pollen with respect to controlgenotypes, and formed an equivalent full seed set (~9% of total seeds) after self-pollination. Flow cytometry analyses of caryopsesderived from antisense lines revealed that all full seeds had originated from meiotic embryo sacs (i.e. by sexuality). A reduction of25.55 % in the germination percentage was detected when comparing antisense lines with controls. Our results indicate that PN_TGS1-like influences ovule, gametophyte and possibly embryo development.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163480
Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Permingeat, Hugo Raúl; Souza Canada, Eduardo Daniel; Siena, Lorena Adelina; et al.; A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules; Frontiers Media; Frontiers in Plant Science; 10; 11-2019; 1-14
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163480
identifier_str_mv Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Permingeat, Hugo Raúl; Souza Canada, Eduardo Daniel; Siena, Lorena Adelina; et al.; A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules; Frontiers Media; Frontiers in Plant Science; 10; 11-2019; 1-14
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2019.01566/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2019.01566
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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