Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics

Autores
Bornancini, Verónica Andrea; Irazoqui, José Matías; Flores, Ceferino René; Vaghi Medina, Carlos Gastón; Amadio, Ariel Fernando; López Lambertini, Paola María
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination.
Fil: Bornancini, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Irazoqui, José Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Flores, Ceferino René. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto.; Argentina
Fil: Vaghi Medina, Carlos Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Amadio, Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: López Lambertini, Paola María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Materia
GEMINIALPHASATELLITINAE
GEMINIVIRIDAE
PEPPER
RCA-NGS
RECOMBINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174646
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/174646
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomicsBornancini, Verónica AndreaIrazoqui, José MatíasFlores, Ceferino RenéVaghi Medina, Carlos GastónAmadio, Ariel FernandoLópez Lambertini, Paola MaríaGEMINIALPHASATELLITINAEGEMINIVIRIDAEPEPPERRCA-NGSRECOMBINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination.Fil: Bornancini, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Irazoqui, José Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Flores, Ceferino René. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto.; ArgentinaFil: Vaghi Medina, Carlos Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Amadio, Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: López Lambertini, Paola María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaMultidisciplinary Digital Publishing Institute2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174646Bornancini, Verónica Andrea; Irazoqui, José Matías; Flores, Ceferino René; Vaghi Medina, Carlos Gastón; Amadio, Ariel Fernando; et al.; Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics; Multidisciplinary Digital Publishing Institute; Viruses; 12; 2; 2-2020; 1-201999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/12/2/202info:eu-repo/semantics/altIdentifier/doi/10.3390/v12020202info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:08Zoai:ri.conicet.gov.ar:11336/174646instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:09.024CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
title Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
spellingShingle Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
Bornancini, Verónica Andrea
GEMINIALPHASATELLITINAE
GEMINIVIRIDAE
PEPPER
RCA-NGS
RECOMBINATION
title_short Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
title_full Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
title_fullStr Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
title_full_unstemmed Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
title_sort Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
dc.creator.none.fl_str_mv Bornancini, Verónica Andrea
Irazoqui, José Matías
Flores, Ceferino René
Vaghi Medina, Carlos Gastón
Amadio, Ariel Fernando
López Lambertini, Paola María
author Bornancini, Verónica Andrea
author_facet Bornancini, Verónica Andrea
Irazoqui, José Matías
Flores, Ceferino René
Vaghi Medina, Carlos Gastón
Amadio, Ariel Fernando
López Lambertini, Paola María
author_role author
author2 Irazoqui, José Matías
Flores, Ceferino René
Vaghi Medina, Carlos Gastón
Amadio, Ariel Fernando
López Lambertini, Paola María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GEMINIALPHASATELLITINAE
GEMINIVIRIDAE
PEPPER
RCA-NGS
RECOMBINATION
topic GEMINIALPHASATELLITINAE
GEMINIVIRIDAE
PEPPER
RCA-NGS
RECOMBINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination.
Fil: Bornancini, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Irazoqui, José Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Flores, Ceferino René. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Salta-jujuy. Estacion Experimental Agropecuaria Yuto.; Argentina
Fil: Vaghi Medina, Carlos Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Amadio, Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: López Lambertini, Paola María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
description In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174646
Bornancini, Verónica Andrea; Irazoqui, José Matías; Flores, Ceferino René; Vaghi Medina, Carlos Gastón; Amadio, Ariel Fernando; et al.; Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics; Multidisciplinary Digital Publishing Institute; Viruses; 12; 2; 2-2020; 1-20
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174646
identifier_str_mv Bornancini, Verónica Andrea; Irazoqui, José Matías; Flores, Ceferino René; Vaghi Medina, Carlos Gastón; Amadio, Ariel Fernando; et al.; Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics; Multidisciplinary Digital Publishing Institute; Viruses; 12; 2; 2-2020; 1-20
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/12/2/202
info:eu-repo/semantics/altIdentifier/doi/10.3390/v12020202
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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