Study of diversity in a set of lentil RILs using morphological and molecular markers

Autores
Bermejo, Carolina Julieta; Gatti, Ileana; Caballero, Nancy; Cravero, Vanina Pamela; Martin, Eugenia Alejandra; Cointry Peix, Enrique Luis
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We propose the use of SRAP and SSR markers along agronomic or morphological traits in genotypes’ differentiation during breeding programs. 25 F5 recombinant inbred lines (microsperma and macrosperma) with good quality characteristics and high yield and a tester were used. All materials were sowed in a randomized block design with 3 replications, at a density of 140 plants per m2 . Different morphological traits were recorded and analyzed using ANOVA. Euclidean distances were calculated and a cluster analysis and a principal components analysis (PCA) were carried out. A total of 20 out of 25 SRAP combinations and 9 out of 17 SSR were assayed on the basis of their high polymorphism. Genetic distances were calculated according to Dice’s similarity index and a dendrogram was performed. A comparison between morphological and molecular data was carried out through the procrustes generalized analysis. The analysis of variance for morphological traits showed significant differences between genotypes for all variables. The analysis of principal components determined that two PCs explained 75% of the total variation observed among the genotypes and the cluster analysis allowed the conformation of two principal groups. The first one included four varieties belonging to the microsperma type, whereas the second group included all the macrosperma type Rils. Both groups showed differences also in yield and plant height. Molecular analysis showed that 66% out of 273 bands are polymorphic fragments from SRAP markers, indicating a high genetic diversity among Rils. The number of alleles per SSR locus ranged from 3 (SSR 33) to 7 (SSR 156) showing also the variability present. Two exclusive SRAP fragments generated by the combination me5-em1 (230 bp) were found in microsperma group allowing early selection of this trait and all characters associated with it. The correlation between Dice similarity index and Euclidean distance matrices was 87% indicating an excellent consistency between both types of markers and suggested that both types of traits would provide similar estimates on the variability and would be useful to differentiate Rils with similar origin
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gatti, Ileana. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
Fil: Caballero, Nancy. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
Fil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
Materia
GENETIC DISTANCE
MOLECULAR MARKERS
MORPHOLOGICAL MARKERS
VARIETIES SELECTION
SRAP
SSR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30114
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30114
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of diversity in a set of lentil RILs using morphological and molecular markersBermejo, Carolina JulietaGatti, IleanaCaballero, NancyCravero, Vanina PamelaMartin, Eugenia AlejandraCointry Peix, Enrique LuisGENETIC DISTANCEMOLECULAR MARKERSMORPHOLOGICAL MARKERSVARIETIES SELECTIONSRAPSSRhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4We propose the use of SRAP and SSR markers along agronomic or morphological traits in genotypes’ differentiation during breeding programs. 25 F5 recombinant inbred lines (microsperma and macrosperma) with good quality characteristics and high yield and a tester were used. All materials were sowed in a randomized block design with 3 replications, at a density of 140 plants per m2 . Different morphological traits were recorded and analyzed using ANOVA. Euclidean distances were calculated and a cluster analysis and a principal components analysis (PCA) were carried out. A total of 20 out of 25 SRAP combinations and 9 out of 17 SSR were assayed on the basis of their high polymorphism. Genetic distances were calculated according to Dice’s similarity index and a dendrogram was performed. A comparison between morphological and molecular data was carried out through the procrustes generalized analysis. The analysis of variance for morphological traits showed significant differences between genotypes for all variables. The analysis of principal components determined that two PCs explained 75% of the total variation observed among the genotypes and the cluster analysis allowed the conformation of two principal groups. The first one included four varieties belonging to the microsperma type, whereas the second group included all the macrosperma type Rils. Both groups showed differences also in yield and plant height. Molecular analysis showed that 66% out of 273 bands are polymorphic fragments from SRAP markers, indicating a high genetic diversity among Rils. The number of alleles per SSR locus ranged from 3 (SSR 33) to 7 (SSR 156) showing also the variability present. Two exclusive SRAP fragments generated by the combination me5-em1 (230 bp) were found in microsperma group allowing early selection of this trait and all characters associated with it. The correlation between Dice similarity index and Euclidean distance matrices was 87% indicating an excellent consistency between both types of markers and suggested that both types of traits would provide similar estimates on the variability and would be useful to differentiate Rils with similar originFil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gatti, Ileana. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; ArgentinaFil: Caballero, Nancy. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; ArgentinaFil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; ArgentinaSouthern Cross Publ2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30114Bermejo, Carolina Julieta; Gatti, Ileana; Caballero, Nancy; Cravero, Vanina Pamela; Martin, Eugenia Alejandra; et al.; Study of diversity in a set of lentil RILs using morphological and molecular markers; Southern Cross Publ; Australian Journal Of Crop Science; 8; 5; 5-2014; 689-6961835-26931835-2707CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cropj.com/berjeo_8_5_2014_689_696.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:22Zoai:ri.conicet.gov.ar:11336/30114instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:22.352CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of diversity in a set of lentil RILs using morphological and molecular markers
title Study of diversity in a set of lentil RILs using morphological and molecular markers
spellingShingle Study of diversity in a set of lentil RILs using morphological and molecular markers
Bermejo, Carolina Julieta
GENETIC DISTANCE
MOLECULAR MARKERS
MORPHOLOGICAL MARKERS
VARIETIES SELECTION
SRAP
SSR
title_short Study of diversity in a set of lentil RILs using morphological and molecular markers
title_full Study of diversity in a set of lentil RILs using morphological and molecular markers
title_fullStr Study of diversity in a set of lentil RILs using morphological and molecular markers
title_full_unstemmed Study of diversity in a set of lentil RILs using morphological and molecular markers
title_sort Study of diversity in a set of lentil RILs using morphological and molecular markers
dc.creator.none.fl_str_mv Bermejo, Carolina Julieta
Gatti, Ileana
Caballero, Nancy
Cravero, Vanina Pamela
Martin, Eugenia Alejandra
Cointry Peix, Enrique Luis
author Bermejo, Carolina Julieta
author_facet Bermejo, Carolina Julieta
Gatti, Ileana
Caballero, Nancy
Cravero, Vanina Pamela
Martin, Eugenia Alejandra
Cointry Peix, Enrique Luis
author_role author
author2 Gatti, Ileana
Caballero, Nancy
Cravero, Vanina Pamela
Martin, Eugenia Alejandra
Cointry Peix, Enrique Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GENETIC DISTANCE
MOLECULAR MARKERS
MORPHOLOGICAL MARKERS
VARIETIES SELECTION
SRAP
SSR
topic GENETIC DISTANCE
MOLECULAR MARKERS
MORPHOLOGICAL MARKERS
VARIETIES SELECTION
SRAP
SSR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We propose the use of SRAP and SSR markers along agronomic or morphological traits in genotypes’ differentiation during breeding programs. 25 F5 recombinant inbred lines (microsperma and macrosperma) with good quality characteristics and high yield and a tester were used. All materials were sowed in a randomized block design with 3 replications, at a density of 140 plants per m2 . Different morphological traits were recorded and analyzed using ANOVA. Euclidean distances were calculated and a cluster analysis and a principal components analysis (PCA) were carried out. A total of 20 out of 25 SRAP combinations and 9 out of 17 SSR were assayed on the basis of their high polymorphism. Genetic distances were calculated according to Dice’s similarity index and a dendrogram was performed. A comparison between morphological and molecular data was carried out through the procrustes generalized analysis. The analysis of variance for morphological traits showed significant differences between genotypes for all variables. The analysis of principal components determined that two PCs explained 75% of the total variation observed among the genotypes and the cluster analysis allowed the conformation of two principal groups. The first one included four varieties belonging to the microsperma type, whereas the second group included all the macrosperma type Rils. Both groups showed differences also in yield and plant height. Molecular analysis showed that 66% out of 273 bands are polymorphic fragments from SRAP markers, indicating a high genetic diversity among Rils. The number of alleles per SSR locus ranged from 3 (SSR 33) to 7 (SSR 156) showing also the variability present. Two exclusive SRAP fragments generated by the combination me5-em1 (230 bp) were found in microsperma group allowing early selection of this trait and all characters associated with it. The correlation between Dice similarity index and Euclidean distance matrices was 87% indicating an excellent consistency between both types of markers and suggested that both types of traits would provide similar estimates on the variability and would be useful to differentiate Rils with similar origin
Fil: Bermejo, Carolina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gatti, Ileana. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
Fil: Caballero, Nancy. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
Fil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martin, Eugenia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cointry Peix, Enrique Luis. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Producción Vegetal. Cátedra de Mejoramiento Vegetal y Prod.de Semillas; Argentina
description We propose the use of SRAP and SSR markers along agronomic or morphological traits in genotypes’ differentiation during breeding programs. 25 F5 recombinant inbred lines (microsperma and macrosperma) with good quality characteristics and high yield and a tester were used. All materials were sowed in a randomized block design with 3 replications, at a density of 140 plants per m2 . Different morphological traits were recorded and analyzed using ANOVA. Euclidean distances were calculated and a cluster analysis and a principal components analysis (PCA) were carried out. A total of 20 out of 25 SRAP combinations and 9 out of 17 SSR were assayed on the basis of their high polymorphism. Genetic distances were calculated according to Dice’s similarity index and a dendrogram was performed. A comparison between morphological and molecular data was carried out through the procrustes generalized analysis. The analysis of variance for morphological traits showed significant differences between genotypes for all variables. The analysis of principal components determined that two PCs explained 75% of the total variation observed among the genotypes and the cluster analysis allowed the conformation of two principal groups. The first one included four varieties belonging to the microsperma type, whereas the second group included all the macrosperma type Rils. Both groups showed differences also in yield and plant height. Molecular analysis showed that 66% out of 273 bands are polymorphic fragments from SRAP markers, indicating a high genetic diversity among Rils. The number of alleles per SSR locus ranged from 3 (SSR 33) to 7 (SSR 156) showing also the variability present. Two exclusive SRAP fragments generated by the combination me5-em1 (230 bp) were found in microsperma group allowing early selection of this trait and all characters associated with it. The correlation between Dice similarity index and Euclidean distance matrices was 87% indicating an excellent consistency between both types of markers and suggested that both types of traits would provide similar estimates on the variability and would be useful to differentiate Rils with similar origin
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30114
Bermejo, Carolina Julieta; Gatti, Ileana; Caballero, Nancy; Cravero, Vanina Pamela; Martin, Eugenia Alejandra; et al.; Study of diversity in a set of lentil RILs using morphological and molecular markers; Southern Cross Publ; Australian Journal Of Crop Science; 8; 5; 5-2014; 689-696
1835-2693
1835-2707
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30114
identifier_str_mv Bermejo, Carolina Julieta; Gatti, Ileana; Caballero, Nancy; Cravero, Vanina Pamela; Martin, Eugenia Alejandra; et al.; Study of diversity in a set of lentil RILs using morphological and molecular markers; Southern Cross Publ; Australian Journal Of Crop Science; 8; 5; 5-2014; 689-696
1835-2693
1835-2707
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cropj.com/berjeo_8_5_2014_689_696.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Southern Cross Publ
publisher.none.fl_str_mv Southern Cross Publ
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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